刚地弓形虫Ⅰ/Ⅲ型ROP16调控TAF15对THP-1细胞影响及机制研究

doi:10.12140/j.issn.1000-7423.2025.01.016

中国寄生虫学与寄生虫病杂志 ›› 2025, Vol. 43 ›› Issue (1): 103-111.doi: 10.12140/j.issn.1000-7423.2025.01.016

刚地弓形虫Ⅰ/Ⅲ型ROP16调控TAF15对THP-1细胞影响及机制研究

殷荷¹^,²(), 马磊³, 党甜甜¹^,², 李佳铭¹^,², 赵志军¹^,²^,^*()

1 宁夏医科大学总医院医学实验中心，宁夏银川 750004
2 宁夏医学检验临床医学研究中心，宁夏银川 750004
3 宁夏大学生命科学学院，宁夏银川 750004

收稿日期:2024-06-24 修回日期:2024-10-16 出版日期:2025-02-28 发布日期:2025-03-26
通讯作者: *赵志军，男，博士，研究员，从事微生物与感染性疾病的研究。E-mail：z15815z@163.com
作者简介:殷荷，女，硕士，技师，从事弓形虫致病机制的研究。E-mail：1573928868@qq.com
基金资助:
宁夏自然科学基金(2024AAC03696);宁夏回族自治区重点研发计划(2023BEG02002)

Effect and mechanism of Toxoplasma gondii ROP16 Ⅰ/Ⅲ regulating TAF15 on THP-1 cells

YIN He¹^,²(), MA Lei³, DANG Tiantian¹^,², LI Jiaming¹^,², Zhao Zhijun¹^,²^,^*()

1 General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China
2 Ningxia Medical Laboratory Clinical Research Center, Yinchuan 750004, Ningxia, China
3 College of Life Sciences of Ningxia University, Yinchuan 750004, Ningxia, China

Received:2024-06-24 Revised:2024-10-16 Online:2025-02-28 Published:2025-03-26
Contact: *E-mail: z15815z@163.com
Supported by:
Ningxia Natural Science Foundation Project(2024AAC03696);Key Research and Development Program of Ningxia Hui Autonomous Region(2023BEG02002)

摘要/Abstract

摘要：

目的探究刚地弓形虫Ⅰ、Ⅲ型棒状体蛋白16（ROP16）通过调控TATA结合蛋白相关因子15（TAF15）对人单核细胞白血病细胞（THP-1）增殖、凋亡的影响及作用机制。方法将Ⅰ、Ⅲ型ROP16过表达慢病毒转染至THP-1细胞，构建稳定表达ROP16的细胞株（THP-1-ROP16Ⅰ/Ⅲ），以转染空载体慢病毒的细胞为空载体对照组（THP-1-Venus），未转染细胞为对照组（THP-1），通过实时荧光定量逆转录PCR（RT-qPCR）和蛋白质免疫印迹（Western blotting）验证过表达效果。利用免疫沉淀-质谱联用技术（IP-MS）在THP-1-ROP16Ⅰ/Ⅲ细胞株中预测ROP16的互作蛋白，并通过RT-qPCR和Western blotting检测细胞中互作蛋白TAF15的表达。将3条作用于TAF15基因不同位点的小干扰RNA（siRNA 1215、siRNA 825、siRNA 288）分别干扰THP-1-ROP16Ⅰ/Ⅲ细胞株，分为THP-1-ROP16Ⅰ/Ⅲ + siRNA 1215/825/288组，同时设未干扰对照组（THP-1-ROP16Ⅰ/Ⅲ + siRNA NC），Western blotting验证TAF15沉默效果。通过细胞计数试剂盒8（CCK-8）与流式细胞术检测各组细胞增殖及凋亡情况，Western blotting检测细胞周期依赖性蛋白激酶抑制因子1A（p21）、周期素依赖性激酶6（CDK6）、G1/S特异性周期蛋白（CyclinD1）、B细胞淋巴瘤/白血病-2蛋白（Bcl-2）、Bcl-2相关X蛋白（Bax）、裂解型半胱氨酸蛋白酶-3（cleaved caspase-3）、半胱氨酸蛋白酶-9（caspase-9）及磷酸化信号转导和转录激活因子3（P-STAT3）蛋白的表达。结果 THP-1-ROP16Ⅰ/Ⅲ组ROP16 mRNA的相对转录水平为2 679.427 ± 250.600、2 395.410 ± 325.700，均高于THP-1-Venus组（1.036 ± 0.102）（F = 153.3，P < 0.01）；ROP16蛋白的相对表达水平为4.526 ± 0.020、5.457 ± 0.250，均高于THP-1-Venus组（1.688 ± 0.653）（F = 76.4，P < 0.01）。TAF15为Ⅰ、Ⅲ型ROP16的互作蛋白，THP-1-ROP16Ⅰ/Ⅲ组TAF15 mRNA的相对转录水平为6.027 ± 0.313、5.567 ± 0.088，均高于THP-1-Venus组（0.985 ± 0.027）（F = 869.4，P < 0.01）；TAF15蛋白的相对表达水平为1.789 ± 0.145、1.593 ± 0.029，均高于THP-1-Venus组（1.010 ± 0.365）（F = 50.6，P < 0.01）。TAF15 siRNA转染48 h后，THP-1-ROP16Ⅰ + siRNA 1215/825/288组TAF15蛋白的相对表达水平分别为0.384 ± 0.047、0.246 ± 0.072、0.125 ± 0.026，均低于THP-1-Venus组（1.007 ± 0.019）（F = 313.1，P < 0.01）；THP-1-ROP16Ⅲ + siRNA 1215/825/288组细胞TAF15蛋白的相对表达水平分别为0.186 ± 0.020、0.180 ± 0.015、0.112 ± 0.019，均低于THP-1-Venus组（0.995 ± 0.052）（F = 3046.0，P < 0.01）。THP-1-ROP16Ⅰ/Ⅲ + siRNA NC组细胞CCK-8实验A₄₅₀值分别为0.803 ± 0.015、0.813 ± 0.011，低于THP-1-Venus组（0.997 ± 0.010、0.995 ± 0.016）（t = 19.2、24.0，均P < 0.01）；THP-1-ROP16Ⅰ/Ⅲ + siRNA 825/288组A₄₅₀值分别为0.986 ± 0.010、0.983 ± 0.004，0.980 ± 0.006、0.984 ± 0.010（F = 3.5、2.9，均P > 0.05）。THP-1-ROP16Ⅰ/Ⅲ + siRNA NC组细胞凋亡率分别为（38.19 ± 0.45）%、（38.06 ± 0.84）%，高于THP-1-Venus组的（28.41 ± 0.69）%（t = 20.5、17.7，均P < 0.01）；THP-1-ROP16Ⅰ/Ⅲ + siRNA 825/288组分别为（30.03 ± 1.83）%、（28.78 ± 0.72）%，（29.33 ± 0.80）%、（28.94 ± 0.58）%（F = 1.5、0.4，均P > 0.05）。THP-1-ROP16Ⅰ + siRNA NC组p21、Bax、caspase-9、cleaved caspase-3及P-STAT3蛋白的相对表达水平分别为1.322 ± 0.027、1.493 ± 0.030、1.349 ± 0.021、1.324 ± 0.020、10.500 ± 1.005，均高于THP-1-Venus组（1.000 ± 0.026、0.996 ± 0.016、0.989 ± 0.019、0.994 ± 0.010、1.000 ± 0.001）（t = 14.8、25.4、22.3、25.0、15.6，均P < 0.01）；CDK6、CyclinD1及Bcl-2蛋白的相对表达水平分别为0.387 ± 0.040、0.424 ± 0.030、0.438 ± 0.035，均低于THP-1-Venus组（0.989 ± 0.018、1.000 ± 0.074、0.991 ± 0.016）（t = 23.6、12.4、25.0，均P < 0.01）。THP-1-ROP16Ⅲ + siRNA NC组p21、Bax、caspase-9、cleaved caspase-3及P-STAT3蛋白的相对表达水平分别为1.409 ± 0.020、1.493 ± 0.030、1.349 ± 0.021、1.324 ± 0.020、16.210 ± 0.664，均高于THP-1-Venus组（1.004 ± 0.032、0.996 ± 0.015、0.989 ± 0.019、0.994 ± 0.010、1.000 ± 0.001）（t = 18.7、25.4、22.3、25.0、39.7，均P < 0.01）；CDK6、CyclinD1及Bcl-2蛋白的相对表达水平分别为0.418 ± 0.021、0.357 ± 0.040、0.411 ± 0.019，均低于THP-1-Venus组（1.000 ± 0.001、1.001 ± 0.042、0.991 ± 0.016）（t = 47.7、19.1、40.7，均P < 0.01）。结论弓形虫Ⅰ、Ⅲ型ROP16蛋白可通过促进TAF15的表达抑制THP-1细胞增殖、促进细胞凋亡，其作用机制可能与抑制细胞内STAT3信号通路的活化有关。

关键词: 刚地弓形虫, 棒状体蛋白16, TATA结合蛋白相关因子15, THP-1细胞

Abstract:

Objective To investigate the effect and mechanisms of Toxoplasma gondii type Ⅰ and Ⅲ rhoptry protein 16 (ROP16) on the proliferation and apoptosis of human monocytic leukemia THP-1 cells via TATA-binding protein-associated factor 15 (TAF15). Methods THP-1 cells were transfected with entiviruses overexpressing T. gondii type Ⅰ and Ⅲ ROP16 to generate cell lines that stably expressed ROP16 (THP-1-ROP16 Ⅰ/Ⅲ), and cell transfected with lentiviruses containing empty vectors (THP-1-Venus) served as an empty vector control, while non-transfected cells (THP-1) served as controls. The efficiency of overexpression was checked using quantitative real-time PCR (RT-qPCR) assay and Western blotting. The proteins interacting with ROP16 were identified using immunoprecipitation-mass spectrometry (IP-MS) in THP-1-ROP16 Ⅰ/Ⅲ cell lines, and the expression of the ROP16-interacting protein TAF15 was quantified using RT-qPCR and Western blotting assays. Three siRNA targeting different sites of TAF15 gene (siRNA 1215, siRNA 825, siRNA 288) were used to interfere with THP-1-ROP16 Ⅰ/Ⅲ cell lines and divided into THP-1-ROP16 Ⅰ/Ⅲ + siRNA 1215/825/288 groups, while an undisturbed control group (THP-1-ROP16 Ⅰ/Ⅲ + siRNA NC) was set up and the silencing efficiency of TAF15 was checked using Western blotting. In addition, the cell proliferation and apoptosis was measured using cell counting kit-8 (CCK-8) assay and flow cytometry, and the expression of cyclin-dependent kinase inhibitor 1A (p21), cyclin-dependent kinase 6 (CDK6), G1/S-specific cyclin (CyclinD1), B-cell lymphoma/leukemia-2 protein (Bcl-2), Bcl-2-associated X protein (Bax), cleaved caspase-3, caspase-9 and phosphorylated signal transducer and activator of transcription 3 (P-STAT3) was determined using Western blotting. Results The relative ROP16 mRNA expression was 2 679.427 ± 250.600 in the THP-1-ROP16 Ⅰ group and 2 395.410 ± 325.700 in the THP-1-ROP16 Ⅲ group, which was both higher than in the THP-1-Venus group (1.036 ± 0.102) (F = 153.3, P < 0.01), and the relative ROP16 protein expression was higher in the THP-1-ROP16 Ⅰ group (4.526 ± 0.020) and THP-1-ROP16 Ⅲ group (5.457 ± 0.250) than in the THP-1 Venus group (1.688 ± 0.653) (F = 76.4, P < 0.01). TAF15 was identified as a protein interacting with type Ⅰ and Ⅲ ROP16, and the relative TAF15 mRNA and protein expression was both higher in the THP-1 ROP16 Ⅰ group (6.027 ± 0.313 and 1.789 ± 0.145) and THP-1 ROP16 Ⅲ group (5.567 ± 0.088 and 1.593 ± 0.029) than in the THP-1 Venus group (0.985 ± 0.027 and 1.010 ± 0.365) (F = 869.4 and 50.6, P < 0.01). The relative TAF15 protein expression was 0.384 ± 0.047, 0.246 ± 0.072, and 0.125 ± 0.026 in the THP-1 ROP16 Ⅰ + siRNA 1215 group, the THP-1 ROP16 Ⅰ + siRNA 825 group, and the THP-1 ROP16 Ⅰ + siRNA 288 group 48 hours post-transfection with TAF15 siRNA, which was all lower than in the THP-1-Venus group (1.007 ± 0.019) (F = 313.1, P < 0.01), and the relative TAF15 protein expression was 0.186 ± 0.020, 0.180 ± 0.015, and 0.112 ± 0.019 in the THP-1 ROP16 Ⅲ + siRNA 1215 group, the THP-1 ROP16 Ⅲ + siRNA 825 group, and the THP-1 ROP16 Ⅲ + siRNA 288 group 48 hours post-transfection with TAF15 siRNA, which was all lower than in the THP-1 Venus group (0.995 ± 0.052) (F = 3 046.0, P < 0.01). CCK-8 assay measured the A₄₅₀ values of 0.803 ± 0.015 and 0.813 ± 0.011 in the THP-1-ROP16 Ⅰ + siRNA NC group and the THP-1-ROP16 Ⅲ + siRNA NC group, which were both lower than in the THP-1 Venus group (0.997 ± 0.010 and 0.995 ± 0.016) (t = 19.2 and 24.0, both P < 0.01). A₄₅₀ values of THP-1-ROP16 Ⅰ/Ⅲ + siRNA 825/288 groups were 0.986 ± 0.010, 0.983 ± 0.004; 0.980 ± 0.006, 0.984 ± 0.010 (F = 3.5, 2.9; both P > 0.05), respectively. The apoptotic rates of THP-1 cells were (38.19 ± 0.45)% in the THP-1-ROP16 Ⅰ + siRNA NC group and (38.06 ± 0.84)% in the THP-1-ROP16 Ⅲ + siRNA NC group, which were both higher than in the THP-1-Venus group [(28.41 ± 0.69)% ] (t = 20.5 and 17.7; both P < 0.01). The apoptotic rates of THP-1-ROP16 Ⅰ/Ⅲ + siRNA 825/288 group were (30.03 ± 1.83)%, (28.78 ± 0.72)%; (29.33 ± 0.80)%, (28.94 ± 0.58)% (F = 1.5, 0.4，both P > 0.05). The relative expression of p21, Bax, Caspase-9, Cleaved Caspase-3, and P-STAT3 proteins was 1.322 ± 0.027, 1.493 ± 0.030, 1.349 ± 0.021, 1.324 ± 0.020, and 10.500 ± 1.005 in the THP-1-ROP16 Ⅰ + siRNA NC group, which was all higher than in the THP-1-Venus group (1.000 ± 0.026, 0.996 ± 0.016, 0.989 ± 0.019, 0.994 ± 0.010 and 1.000 ± 0.001) (t = 14.8, 25.4, 22.3, 25.0 and 15.6; all P < 0.01), and the relative CDK6, CyclinD1, and Bcl-2 protein expression was 0.387 ± 0.040, 0.424 ± 0.030, and 0.438 ± 0.035 in the THP-1-ROP16 Ⅰ + siRNA NC group, which was all lower than in the THP-1-Venus group (0.989 ± 0.018, 1.000 ± 0.074 and 0.991 ± 0.016) (t = 23.6, 12.4 and 25.0; all P < 0.01). The relative expression of p21, Bax, caspase-9, cleaved caspase-3, and P-STAT3 proteins was 1.409 ± 0.020, 1.493 ± 0.030, 1.349 ± 0.021, 1.324 ± 0.020, and 16.210 ± 0.664 in the THP-1-ROP16 Ⅲ + siRNA NC group, which was all higher than in the THP-1-Venus group (1.004 ± 0.032, 0.996 ± 0.015, 0.989 ± 0.019, 0.994 ± 0.010 and 1.000 ± 0.001) (t = 18.7, 25.4, 22.3, 25.0 and 39.7; all P < 0.01), and the relative expression of CDK6, CyclinD1, and Bcl-2 proteins was 0.418 ± 0.021, 0.357 ± 0.040, and 0.411 ± 0.019 in the THP-1-ROP16 Ⅲ + siRNA NC group, which was all lower than in the THP-1-Venus group (1.000 ± 0.001, 1.001 ± 0.042 and 0.991 ± 0.016) (t = 47.7, 19.1 and 40.7; all P < 0.01). Conclusion T. gondii type Ⅰ and Ⅲ ROP16 proteins inhibit THP-1 cell proliferation and promote cell apoptosis through promoting TAF15 expression, which may be associated with inhibition of activation of the STAT3 signaling pathway in THP-1 cells.

Key words: Toxoplasma gondii, ROP16 protein, TAF15, THP-1 cell

中图分类号:

R382.5

殷荷, 马磊, 党甜甜, 李佳铭, 赵志军. 刚地弓形虫Ⅰ/Ⅲ型ROP16调控TAF15对THP-1细胞影响及机制研究[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(1): 103-111.

YIN He, MA Lei, DANG Tiantian, LI Jiaming, Zhao Zhijun. Effect and mechanism of Toxoplasma gondii ROP16 Ⅰ/Ⅲ regulating TAF15 on THP-1 cells[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2025, 43(1): 103-111.

图/表 8

表1

表2

图1

图2

图3

图4

图5

图6

参考文献 20

[1]	Smith NC, Goulart C, Hayward JA, et al. Control of human toxoplasmosis[J]. Int J Parasitol, 2021, 51(2/3): 95-121.
[2]	Rabaan AA, Uzairue LI, Alfaraj AH, et al. Seroprevalence, risk factors and maternal-fetal outcomes of Toxoplasma gondii in pregnant women from WHO eastern Mediterranean Region: Systematic review and meta-analysis[J]. Pathogens, 2023, 12(9): 1157.
[3]	Dubey JP. Outbreaks of clinical toxoplasmosis in humans: Five decades of personal experience, perspectives and lessons learned[J]. Parasit Vectors, 2021, 14(1): 263. doi: 10.1186/s13071-021-04769-4 pmid: 34011387
[4]	Picard C, Macagno N, Corradini N, et al. Identification of a novel translocation producing an in-frame fusion of TAF15 and ETV4 in a case of extraosseous Ewing sarcoma revealed in the prenatal period[J]. Virchows Arch, 2022, 481(4): 665-669.
[5]	Xu LQ, Yao LJ, Jiang D, et al. A uracil auxotroph Toxoplasma gondii exerting immunomodulation to inhibit breast cancer growth and metastasis[J]. Parasit Vectors, 2021, 14(1): 601.
[6]	Ye HM, Zhou XT, Zhu BK, et al. Toxoplasma gondii suppresses proliferation and migration of breast cancer cells by regulating their transcriptome[J]. Cancer Cell Int, 2024, 24(1): 144.
[7]	张富强, 乔姣姣, 李浩然, 等. 刚地弓形虫抗肿瘤作用及其机制研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(4): 496-501, 507. doi: 10.12140/j.issn.1000-7423.2020.04.017
	Zhang FQ, Qiao JJ, Li HR, et al. Research progress on the anti-tumor effects of Toxoplasma gondii and the underlying mechanisms[J]. Chin J Parasitol Parasit Dis, 2020, 38(4): 496-501, 507. (in Chinese)
[8]	Chen JT, Liao WZ, Peng HJ. Toxoplasma gondii infection possibly reverses host immunosuppression to restrain tumor growth[J]. Front Cell Infect Microbiol, 2022, 12: 959300.
[9]	刘功振, 王彬, 王洪法. 弓形虫棒状体蛋白ROP16的研究进展[J]. 中国血吸虫病防治杂志, 2015, 27(2): 217-220.
	Liu GZ, Wang B, Wang HF. Advances in research of Toxoplasma gondii rhoptry protein ROP16[J]. Chin J Schisto Control, 2015, 27(2): 217-220. (in Chinese)
[10]	Chen LF, Christian DA, Kochanowsky JA, et al. The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses[J]. J Exp Med, 2020, 217(3): e20181757.
[11]	刁玉洁, 昌庆琛, 刘珍, 等. 弓形虫ROP16蛋白诱导肝癌细胞凋亡的检测[J]. 肝胆外科杂志, 2016, 24(6): 466-468.
	Diao YJ, Chang QC, Liu Z, et al. Analysis of HepG2 apoptosis induced by Toxoplasma gondii effector protein ROP16[J]. J Hepatobiliary Surg, 2016, 24(6): 466-468. (in Chinese)
[12]	Li GQ, Li QH, Tong YQ, et al. The anticancer mechanisms of Toxoplasma gondii rhoptry protein 16 on lung adenocarcinoma cells[J]. Cancer Biol Ther, 2024, 25(1): 2392902.
[13]	Chang S, Shan XM, Li XL, et al. Toxoplasma gondii rhoptry protein ROP16 mediates partially SH-SY5Y cells apoptosis and cell cycle arrest by directing Ser15/37 phosphorylation of p53[J]. Int J Biol Sci, 2015, 11(10): 1215-1225.
[14]	陈梅, 贾伟, 党甜甜, 等. 弓形虫ROP16蛋白在人白血病细胞THP-1表达及对其细胞增殖与凋亡的影响[J]. 中国人兽共患病学报, 2022, 38(4): 277-284. doi: 10.3969/j.issn.1002-2694.2022.00.041
	Chen M, Jia W, Dang TT, et al. Expression of Toxoplasma gondii ROP16 protein in THP-1 human leukemia cells and its effects on proliferation and apoptosis[J]. Chin J Zoonoses, 2022, 38(4): 277-284. (in Chinese)
[15]	Åman P, Dolatabadi S, Svec D, et al. Regulatory mechanisms, expression levels and proliferation effects of the FUS-DDIT3 fusion oncogene in liposarcoma[J]. J Pathol, 2016, 238(5): 689-699.
[16]	Singh AK, Kapoor V, Thotala D, et al. TAF15 contributes to the radiation-inducible stress response in cancer[J]. Oncotarget, 2020, 11(27): 2647-2659. doi: 10.18632/oncotarget.27663 pmid: 32676166
[17]	Ren P, Xing L, Hong XD, et al. LncRNA PITPNA-AS 1 boosts the proliferation and migration of lung squamous cell carcinoma cells by recruiting TAF15 to stabilize HMGB3 mRNA[J]. Cancer Med, 2020, 9(20): 7706-7716.
[18]	Shamloo B, Usluer S. p21 in cancer research[J]. Cancers, 2019, 11(8): 1178.
[19]	Yang CL, Arrizabalaga G. The serine/threonine phosphatases of apicomplexan parasites[J]. Mol Microbiol, 2017, 106(1): 1-21. doi: 10.1111/mmi.13715 pmid: 28556455
[20]	李佳铭, 王艺璇, 杨宁爱, 等. 刚地弓形虫ROP16蛋白对MH-S细胞极化和凋亡的影响及其相关机制[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 579-586. doi: 10.12140/j.issn.1000-7423.2022.05.003
	Li JM, Wang YX, Yang NA, et al. Effects of ROP16 protein of Toxoplasma gondii on polarization and apoptosis of MH-S cells and their related mechanisms[J]. Chin J Parasitol Parasit Dis, 2022, 40(5): 579-586. (in Chinese)

基因 Gene	上游引物（5′→3′） Forward primer (5′→3′)	下游引物（5′→3′） Reverse primer (5′→3′)
ROP16	CGGCTGAAGTAGGTCTCGG	GGTGAAAGCTGGGTTTGGT
TAF15	GATTCTGGAAGTTACGGT-CAGTC	AGCTTTGTGATGCTTGTCCA-TAG
β-actin	CACTGTGCCCATCTACGA	TGATGTCACGCACGATTT

细胞株 Cell line	蛋白编码 Protein ID	蛋白名称 Protein name	简称 Abbreviation
THP-1-ROP16Ⅰ	P63261	Actin, cytoplasmic 2	ACTG1
	P60709	Actin, cytoplasmic 1	ACTB
	P35637	RNA-binding protein FUS	FUS
	Q92804	TATA-binding protein-associated factor 2N	TAF15
	P49411	Elongation factor Tu，mitochondrial	TUFM
THP-1-ROP16Ⅲ	Q92804	TATA-binding protein-associated factor 2N	TAF15
THP-1-ROP16Ⅲ	P35609	Alpha-actinin-2	ACTN2
	Q08043	Alpha-actinin-3	ACTN3

刚地弓形虫Ⅰ/Ⅲ型ROP16调控TAF15对THP-1细胞影响及机制研究

Effect and mechanism of Toxoplasma gondii ROP16 Ⅰ/Ⅲ regulating TAF15 on THP-1 cells

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 20

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李仕毓, 李静, 陆绍红, 郑斌. 宿主细胞自主免疫抗弓形虫的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(5): 653-658.
[2]	解晓曼, 孙航, 代莉莎, 朱文菊, 王利磊, 谢环环, 董宏杰, 张俊梅, 王琦, 周贝贝, 赵桂华, 徐超, 尹昆. 刚地弓形虫感染对小鼠脑组织转录本m⁶A甲基化修饰的影响[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 27-35.
[3]	郑广福, 刘现兵, 姜昱竹, 李新雨, 胡雪梅, 张海霞. 刚地弓形虫感染孕鼠胎盘组织中中性粒细胞和IL-17与不良妊娠结局的关系[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 48-54.
[4]	薛羽珊, 林萍, 程训佳, 冯萌. 慢性弓形虫感染对宿主中枢神经系统的损伤及其作用机制[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 527-531.
[5]	姜文静, 孟雅莉, 赵利娜, 王春苗, 张晓磊. 刚地弓形虫棒状体蛋白18和膜表面抗原30复合核酸疫苗对小鼠的免疫保护作用[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 532-538.
[6]	赵紫琪, 吕芳丽. 蒿甲醚脂质体体外抑制刚地弓形虫增殖作用的研究[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 446-451.
[7]	张驰, 陈嘉婷, 辛紫萱, 杨莉莉, 杨梓瀚, 彭鸿娟. 弓形虫慢性感染小鼠脑转录组分析及与抑郁相关的犬尿氨酸通路的验证[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 270-278.
[8]	杜鹃, 李佳, 吴迪, 余琦, 张玮, 白如念, 郭俊林, 刘庆斌, 雷琪莉, 谷传慧, 王萌, 赵浩军. 2022年北京市犬猫刚地弓形虫感染血清流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 389-392.
[9]	李佳铭, 王艺璇, 杨宁爱, 马慧慧, 兰敏, 刘春兰, 赵志军. 刚地弓形虫ROP16蛋白对MH-S细胞极化和凋亡的影响及其相关机制[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 579-586.
[10]	邹伟浩, 吴蔚玲, 廖远鹏, 陈敏, 彭鸿娟. 刚地弓形虫抗缓殖子期抗原1单克隆抗体的制备与应用[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 587-593.
[11]	代莉莎, 张丽新, 尹昆. 刚地弓形虫诱导宿主精神行为障碍的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 642-646.
[12]	王杰, 温红阳, 陈滢, 安然, 罗庆礼, 沈继龙, 都建. 刚地弓形虫巨噬细胞迁移抑制因子基因敲除虫株的构建与鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(3): 349-354.
[13]	王振勋, 熊思思, 孙夏慧, 王永亮, 潘格, 何深一, 丛华. 刚地弓形虫慢性感染小鼠脑组织中lncRNA102796的差异表达及其作用机制[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(2): 187-193.
[14]	蒋峰, 陈润, 都宁宁, 朱梦怡, 钟昊, 陈辉, 奚旭霞, 湛孝东, 李朝品. 芜湖市区宠物犬、猫刚地弓形虫感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(1): 124-126.
[15]	鲁飞, 卓洵辉, 陆绍红. 顶复门原虫感染与宿主细胞自噬相互作用的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(6): 826-831.