弓形虫感染鼠肝脾脑微量元素的测定

中国寄生虫学与寄生虫病杂志 ›› 2000, Vol. 18 ›› Issue (6): 8-349.

弓形虫感染鼠肝脾脑微量元素的测定

耿志辉¹;石毅²;方艳秋³;李淑红¹;刘利¹

白求恩医科大学!基础医学院病原生物学教研室;长春130021;白求恩医科大学!生物工程研究所分析研究室;长春130021;白求恩医科大学!第一临床学院中心实验室;长春130021;

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-12-30 发布日期:2000-12-30

ANALYSIS OF TRACE ELEMENTS IN LIVER, SPLEEN AND BRAIN OF RATS INFECTED WITH TOXOPLASMA GONDII

GENG Zhi-hui ¹;SHI Yi ²;FANG Yan-qiu ³;LI Shu-hong ¹;LIU Li ¹

1 Department of Pathogenobiology;Norman Bethune University of Medical Sciences;Changchun 130021; 2 Department of Analysis;Institute of Biotechnology;Norman Bethune University of Medical Sciences;Changchun 130021;3 Laboratory of Centre, the Frist clinical Hospital,Norman Bethune University of Medical Sciences;Changchun 130021;

Received:1900-01-01 Revised:1900-01-01 Online:2000-12-30 Published:2000-12-30

摘要/Abstract

摘要： 　　[目的 ]研究感染弓形虫对大鼠肝、脾及脑组织 5种微量元素 (Fe2 +、Cu2 +、Zn2 +、Ca2 +、Mg2 +)的影响。 [方法 ]2 0只大鼠随机均分为正常组和感染组。每只大鼠经腹腔注入 2ml含 1 5× 10 6弓形虫速殖子的生理盐水悬液 ,6 4d后将 2组大鼠处死 ,取肝、脾、及脑组织 ,用原子吸收分光光度计测定其微量元素的变化。 [结果 ]与正常组相比较 ,感染组肝组织Fe2 +含量明显增加 (P <0 0 1) ,Cu2 +含量明显减少 (P <0 0 5 ) ,而Zn2 +、Ca2 +、Mg2 +的含量变化均无显著差异 ;感染组脾组织Fe2 +、Cu2 +含量明显减少 (P <0 0 1) ,Mg2 +增加 (P <0 0 1) ,Zn2 +、Ca2 +无显著差异 ;感染组脑组织Fe2 +、Cu2 +、Mg2 +含量明显减少 (P <0 0 1) ,Ca2 +增加 (P <0 0 2 ) ,Zn2 +无明显变化。 [结论 ]弓形虫感染能引起大鼠肝、脾及脑组织微量元素的改变

关键词: 刚地弓形虫, 微量元素, 肝脏, 脾脏, 脑, 大鼠

Abstract: 　Objective] To study the level of five trace elements(Fe 2+ 、Cu 2+ 、Zn 2+ 、Ca 2+ 、Mg 2+ )in the liver, spleen and brain of the rats infected with Toxoplasma gondii. [Methods] 20 rats were randomly and equally divided into two groups: normal group and experimental group. On the 64th day after injection of 1.5×106T.gondii/2 ml, all of the rats were killed and the atomic absorption method were used. [Results] Compared with the normal group, the level of Cu 2+ in liver in experimental group was significantly reduced(P<0 01), and the amount of Fe 2+ of sick rats were higher than that of the normal(P<0 01). No differences in the content of Zn 2+ 、Ca 2+ 、Mg 2+ between the experimental and control group were determined; the level of Fe 2+ 、Cu 2+ in spleen in experimental group was significantly altered(P<0 01). The amount of Mg 2+ of sick rats were higher than that of the normal (P<0 01), and no difference on the content of Zn 2+ 、Ca 2+ between the experimental and control group were estimated;the level of Fe 2+ 、Cu 2+ 、Mg 2+ in brain in experimental group was significantly reduced(P<0 01).The amount of Ca 2+ of sick rats were higher than that of the normal(P<0 02).No difference on the content of Zn 2+ between the experimental and control group were estimated. [Conclusion] T.gondii infection might cause changes in trace elements in the liver,spleen and brain of rats.

Key words: Toxoplasma gondii, liver, spleen, brain, trace element, rat

耿志辉;石毅;方艳秋;李淑红;刘利. 弓形虫感染鼠肝脾脑微量元素的测定[J]. 中国寄生虫学与寄生虫病杂志, 2000, 18(6): 8-349.

GENGZhi-hui;SHIYi;FANGYan-qiu;LIShu-hong;LIULi. ANALYSIS OF TRACE ELEMENTS IN LIVER, SPLEEN AND BRAIN OF RATS INFECTED WITH TOXOPLASMA GONDII[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2000, 18(6): 8-349.

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