弓形虫病大鼠T淋巴细胞和脾微量元素的测定

中国寄生虫学与寄生虫病杂志 ›› 2001, Vol. 19 ›› Issue (6): 11-359.

弓形虫病大鼠T淋巴细胞和脾微量元素的测定

耿志辉;方艳秋;刘利;石毅;李淑红

白求恩医科大学基础医学院第三综合实验室长春130021 (耿志辉);白求恩医科大学第一临床学院中心实验室长春130021 (方艳秋);白求恩医科大学基础医学院病原生物教研室长春130021 (刘利);白求恩医科大学生物工程研究所分析研究室长春130021 (石毅);白求恩医科大学基础医学院病原生物教研室长春130021(李淑红)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-12-30 发布日期:2001-12-30

Determination of T Lymphocytes and Trace Elements in Spleen from Rats Infected with Toxoplasma gondii

GENG Zhi hui 1;FANG Yan qiu 2;LIU Li 3;SHI Yi 4;LI Shu hong 3

1 Department of the Third Centre; 2 Central Laboratory;the First Clinical Hospital; 3 Department of Pathobiology; 4 Department of Analysis;Institute of Biotechnology;Norman

Received:1900-01-01 Revised:1900-01-01 Online:2001-12-30 Published:2001-12-30

摘要/Abstract

摘要： 　　目的　观察感染弓形虫大鼠外周血T淋巴细胞及其亚型以及脾组织中 5种微量元素 (Fe2 + 、Cu2 + 、Zn2 + 、Ca2 + 、Mg2 + )含量的变化。方法　 2 0只大鼠随机分为实验组和对照组。实验组每鼠经腹腔注入 2ml含 1.5× 10 6弓形虫速殖子的生理盐水悬液 ,对照组每鼠腹腔注射 2ml生理盐水。 6 4d后将 2组大鼠处死 ,取外周血及脾组织。用细胞内α 醋酸萘酯酶标记T淋巴细胞的方法观察弓形虫病大鼠外周血T淋巴细胞 (TL)及其亚型辅助性T淋巴细胞 (Th)和抑制性T淋巴细胞 (Ts)的变化 ,同时用原子吸收分光光度仪测定其微量元素的变化。结果　实验组外周血TL和Th均低于正常对照组 (P <0 .0 1、P <0 .0 1) ,两组间Th/Ts比值差异显著 (P <0 .0 1) ;实验组脾Fe2 + 、Cu2 + 含量明显减少 (P <0 .0 1,P <0 .0 1) ,而Mg2 + 含量明显增加 (P <0 .0 1)。结论　弓形虫感染能引起大鼠外周血TL、Th和Ts改变及脾内微量元素的变化

关键词: 弓形虫, 微量元素, 大鼠, α-醋酸萘酯酶, 淋巴细胞

Abstract: 　Objective To determine the level of five trace elements(Fe 2+ ?Cu 2+ ?Zn 2+ ?Ca 2+ ?Mg 2+ )in the spleen and changes of T lymphocyte and its subtype variations in peripheral blood from the rats infected with Toxoplasma gondii . Methods Twenty rats were randomly and equally divided into two groups: control group and experiment group. Each rat in the experiment group received an ip injection of 2 ml normal saline containing 1.5×10 6 tachyzoites of T. gondii . On the 64th day after injection of T.gondii , the changes in T lymphocytes (TL) and their subgroups, the helper T lymphocytes (Th) and the suppressor T lymphocytes(Ts) in the peripheral blood of the rats with T.gondii were determined by the assay of the lymphocytes labeled with intercellular acid α naphthyl acetate esterase. All the rats were killed and the atomic absorption method were used for detecting the level of trace elements in the spleen tissue. Results The number of TL and Th in experiment group was significantly lower than that of control ( P <0.01, P <0.01). The ratio of Th/Ts showed a significant difference between the two groups. The level of Fe 2+ ?Cu 2+ in experiment group was significantly reduced ( P <0.01). The amount of Mg 2+ in infected rats was higher than that of the control( P <0.01). No statistical difference in the content of Zn 2+ ?Ca 2+ was found between the two groups. Conclusion T.gondii infection might cause the changes in the TL and Th in peripheral blood and the changes in trace elements in spleen of the rats.

Key words: Toxoplasma gondii, trace element, rat, acid α-naphthyl acetate esterase, lymphocyte

耿志辉;方艳秋;刘利;石毅;李淑红. 弓形虫病大鼠T淋巴细胞和脾微量元素的测定[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(6): 11-359.

GENGZhihui;FANGYanqiu;LIULi;SHIYi;LIShuhong. Determination of T Lymphocytes and Trace Elements in Spleen from Rats Infected with Toxoplasma gondii[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2001, 19(6): 11-359.

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