上海市市售海鱼异尖线虫幼虫感染情况调查

doi:10.12140/j.issn.1000-7423.2024.01.017

中国寄生虫学与寄生虫病杂志 ›› 2024, Vol. 42 ›› Issue (1): 117-120.doi: 10.12140/j.issn.1000-7423.2024.01.017

上海市市售海鱼异尖线虫幼虫感染情况调查

张瑞¹(), 王子¹, 王佳慧², 李凤琴², 谢庆超¹^,^*(), 赵勇¹

1 上海海洋大学食品学院，农业农村部水产品贮藏保鲜质量安全风险评估实验室（上海），上海水产品加工及贮藏工程技术研究中心，上海海洋大学食品质量安全检测实验室，上海 201306
2 国家食品安全风险评估中心，北京 100022

收稿日期:2023-05-21 修回日期:2023-07-19 出版日期:2024-02-28 发布日期:2024-03-12
通讯作者: *谢庆超（1986—），男，博士，高级工程师，从事食品质量安全风险评估、食品质量安全与系统生物学方向的研究。E-mail：qcxie@shou.edu.cn
作者简介:张瑞（1998—），男，硕士研究生，从事食品质量安全风险评估方向的研究。E-mail：zhangrui2021y@163.com
基金资助:
上海市优秀学术带头人计划(21XD1401200);国家自然科学基金(31972188);上海市科技兴农项目[沪农科创字（2021）第3-2号]

Investigation of Anisakis larvae infection in marine fishes from Shanghai market

ZHANG Rui¹(), WANG Zi¹, WANG Jiahui², LI Fengqin², XIE Qingchao¹^,^*(), ZHAO Yong¹

1 School of Food, Shanghai Ocean University, Risk Assessment Laboratory for Aquatic Product Storage and Preservation Quality and Safety of the Ministry of Agriculture and Rural Affairs (Shanghai), Shanghai Aquatic Product Processing and Storage Engineering Technology Research Center, Shanghai Ocean University Food Quality and Safety Testing Laboratory, Shanghai 201306, China
2 National Food Safety Risk Assessment Center, Beijing 100022, China

Received:2023-05-21 Revised:2023-07-19 Online:2024-02-28 Published:2024-03-12
Contact: *E-mail: qcxie@shou.edu.cn
Supported by:
the Shanghai Excellent Academic Leaders Program(21XD1401200);the National Natural Science Foundation of China(31972188);the Shanghai Science and Technology Promoting Agriculture Project[Hu Nong Ke Chuang Zi(2021) 3-2]

摘要/Abstract

摘要：

为了解上海市市售海鱼异尖线虫幼虫感染情况，2022年在上海市农贸市场、超市和海鲜市场中采集自东海海域捕捞的新鲜海鱼，解剖后分别在内脏和鱼肉中查找疑似异尖线虫的虫体，分别置于光学显微镜和扫描电子显微镜下进行观察，分析海鱼的异尖线虫感染情况。共采集海鱼16种338尾，从6种116尾体内检出异尖线虫1 065条，总感染率为34.3%（116/338），平均感染度为9.2条/尾。其中鮟鱇鱼的感染率最高（11/12），小黄鱼的感染度最高（13.0条/尾）。海鱼异尖线虫感染率从春季到冬季逐渐升高，冬季的感染率和感染度最高，分别为51.1%（46/90）和12.3条/尾。异尖线虫寄生的主要部位是海鱼的肠道和腹腔，分别占检出异尖线虫总数的54.6%（582/1 065）和40.7%（433/1 065）。本研究结果提示，上海市市售海鱼存在异尖线虫幼虫感染，感染率较高的鱼种有鮟鱇鱼、小黄鱼等常见食用海鱼。

关键词: 异尖线虫, 海鱼, 调查

Abstract:

To understand the Anisakis larvae infection status in marine fishes sold in Shanghai, the fresh marine fishes caught in the East China Sea area were collected from Farmers’ markets, supermarkets and seafood markets in Shanghai in 2022. The suspected Anisakis larvae were searched in the offal and muscles after dissection and observed under optical microscope and scanning electron microscope, respectively. A total of 338 marine fish of 16 species were collected, and 1 065 Anisakis larvae were found from 116 fish of 6 species, with a total infection rate of 34.3% (116/338) and average infection intensity of 9.2 larvae/fish. The highest infection rate was 11/12 in Lophiiformes, and the highest average infection intensity was 13.0 larvae/fish in Larimichthys polyactis. The Anisakis larvae infection rates increased gradually from spring to winter. The infection rate and average infection intensity in winter were 51.1% (46/90) and 12.3 larvae/fish, respectively, which were the highest seasons of the year. The predominant sites of Anisakis larvae parasitise in marine fishes were the intestines and abdominal cavity, with infection rates of 54.6% (582/1 065) and 40.7% (433/1 065), respectively. The result showed that Anisakis larvae infections were present in marine fish sold in Shanghai and the infection rates of commonly consumed marine fishes such as Lophiiformes and L. polyactis were high.

Key words: Anisakis spp., Marine fish, Survey

中图分类号:

R532.19

张瑞, 王子, 王佳慧, 李凤琴, 谢庆超, 赵勇. 上海市市售海鱼异尖线虫幼虫感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 117-120.

ZHANG Rui, WANG Zi, WANG Jiahui, LI Fengqin, XIE Qingchao, ZHAO Yong. Investigation of Anisakis larvae infection in marine fishes from Shanghai market[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2024, 42(1): 117-120.

图/表 4

图1

图2

表1

表2

参考文献 17

[1]	Lin CX, Huang SL, Lin SH, et al. Infection and species identification of Anisakis larvae in sea fishes along Fujian coast[J]. Chin J Parasitol Parasit Dis, 2019, 37(4): 417-421. (in Chinese)
	(林陈鑫, 黄姝玲, 林诗涵, 等. 福建省沿海鱼类异尖线虫幼虫感染情况调查及虫种鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(4): 417-421.)
[2]	Zheng D, Lin CX, Cai WW, et al. Investigation of Anisakis spp. infection in marine fish in Fujian Province, 2019—2021[J]. Chin J Parasitol Parasit Dis, 2023, 41(2): 238-240, 244. (in Chinese)
	(郑丹, 林陈鑫, 蔡武卫, 等. 2019—2021年福建省海鱼异尖线虫幼虫感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 238-240, 244.)
[3]	Wei ZC, Zhang W, Deng XL. Investigation of Anisakis larvae infection in marine fish in markets of Anshan City, Liaoning Province[J]. Chin J Parasitol Parasit Dis, 2020, 38(6): 789-791. (in Chinese)
	(魏卓超, 张巍, 邓晓丽. 辽宁省鞍山市市售海鱼异尖线虫幼虫感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(6): 789-791.)
[4]	Wongsawad P, Cheypanya V, Wongsawad C, et al. Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish[J]. Asian Pacific J Trop Med, 2021, 14(5): 214-222.
[5]	Zhu X, Gasser RB, Podolska M, et al. Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences[J]. Int J Parasitol, 1998, 28(12): 1911-1921.
[6]	Guo YN. Identification of ascaridoid nematodes from fourteen vertebrates in China[D]. Shijiazhuang: Hebei Normal University, 2015: 1-4. (in Chinese)
	(郭彦宁. 中国14种脊椎动物寄生蛔虫物种鉴定[D]. 石家庄: 河北师范大学, 2015: 1-4.)
[7]	Ning J, Huang K, Wei BB, et al. Parasitic characteristics of Anisakis sp. in the liver of Gadus chalcogrammus in the Western Bering Sea during summer[J]. Mar Fish, 2020, 42(3): 296-303. (in Chinese)
	(宁静, 黄开, 韦贝贝, 等. 西白令海夏季狭鳕肝脏异尖线虫感染特性研究[J]. 海洋渔业, 2020, 42(3): 296-303.)
[8]	Abattouy N, Valero A, Benajiba MH, et al. Anisakis simplex s.l. parasitization in mackerel (Scomber japonicus) caught in the North of Morocco: Prevalence and analysis of risk factors[J]. Int J Food Microbiol, 2011, 150(2/3): 136-139.
[9]	Nurhidayat SW, Nam UH, Kim JH. Occurrence of anisakid nematodes in walleye pollock (Gadus chalcogrammus) caught off the East Sea of Korea: their molecular identification and biological implication[J]. Ocean Sci J, 2018, 53(4): 679-689.
[10]	Zhu GP, Zheng ZH, Qian HR, et al. Factors impacting infection characteristics of Anisakis sp. in liver of walleye pollock (Gadus chalcogrammus) in the western Bering Sea[J]. J Shanghai Ocean Univ, 2022, 31(2): 471-478. (in Chinese)
	(朱国平, 郑智泓, 钱胡蕊, 等. 西白令海狭鳕肝脏异尖线虫感染特性的影响因素分析[J]. 上海海洋大学学报, 2022, 31(2): 471-478.)
[11]	Pan HJ, Xia RL, Li H, et al. Progress on ecological factors affected fish parasites dynamical changes[J]. Ecol Sci, 2014, 33(6): 1200-1207. (in Chinese)
	(潘厚军, 夏润林, 李华, 等. 鱼类寄生虫动态变化相关生态因子的研究进展[J]. 生态科学, 2014, 33(6): 1200-1207.)
[12]	Andersen PS, Buchmann K. Temperature dependent population growth of Gyrodactylus derjavini on rainbow trout, Oncorhynchus mykiss[J]. J Helminthol, 1998, 72(1): 9-14.
[13]	Peng F, Liu NM, Feng F. Effect of ultraviolet ray radiation on the development of ascaris lumbricoides seminal eggs[J]. Chin Trop Med, 2004, 4(1): 45-46. (in Chinese)
	(彭飞, 刘年猛, 冯芳. 紫外线辐射对受精蛔虫卵发育的影响[J]. 中国热带医学, 2004, 4(1): 45-46.)
[14]	Studer A, Lamare MD, Poulin R. Effects of ultraviolet radiation on the transmission process of an intertidal trematode parasite[J]. Parasitology, 2012, 139(4): 537-546.
[15]	Overholt EP, Hall SR, Williamson CE, et al. Solar radiation decreases parasitism in Daphnia[J]. Ecol Lett, 2012, 15(1): 47-54.
[16]	Chi LB, Song XX, Yuan YQ, et al. Distribution of dissolved oxygen in the Yellow Sea and East China Sea in summer and winter[J]. Oceanol Limnol Sin, 2017, 48(6): 1337-1345. (in Chinese)
	(池连宝, 宋秀贤, 袁涌铨, 等. 夏、冬季黄东海溶解氧的分布特征研究[J]. 海洋与湖沼, 2017, 48(6): 1337-1345.)
[17]	Rizvi A, Alam MM, Parveen S, et al. Abandoning the ship: spontaneous mass exodus of Clinostomum complanatum (Rudolphi, 1814) progenetic metecercariae from the dying intermediate host Trichogaster fasciatus (Bloch & Schneider, 1801)[J]. J Parasit Dis, 2012, 36(1): 139-140.

鱼种	肠道		腹腔		肌肉		性腺		合计
鱼种	检获条数	构成比/%	检获条数	构成比/%	检获条数	构成比/%	检获条数	构成比/%	合计
鮟鱇鱼 Lophiiformes	27	22.9	91	77.1	00	0.	00	0.	1 118
小黄鱼 L. polyactis	406	61.1	225	33.9	24	3.6	09	1.4	1 664
带鱼 T. lepturus	74	42.8	82	47.4	11	6.4	06	3.5	1 173
海鳗 M. cinereus	66	68.8	30	31.3	00	0.	00	0.	1 096
银鲳鱼 P. argenteus	8	8/12	4	4/12	00	0.	00	0.	1 012
梅童鱼 C. niveatus	1	1/2	1	1/2	00	0.	00	0.	1 002
合计	582	54.6	433	40.7	35	3.3	15	1.4	1 065

上海市市售海鱼异尖线虫幼虫感染情况调查

Investigation of Anisakis larvae infection in marine fishes from Shanghai market

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 17

相关文章 15

编辑推荐

Metrics

本文评价

鱼种	检查数/尾	感染数/尾	异尖线虫感染率/%	异尖线虫检获数/条	感染度/条·尾^-1
鮟鱇鱼 Lophiiformes	12	11	11/12	118	10.7
小黄鱼 L. polyactis	69	51	73.9	664	13.0
带鱼 T. lepturus	71	34	47.9	173	05.1
海鳗 M. cinereus	22	10	45.5	96	09.6
银鲳鱼 Pampus argenteus	75	8	10.7	12	01.5
梅童鱼 Collichthys niveatus	22	2	09.1	2	01.0
大黄鱼 Larimichthys crocea	4	0	00	0	00
马鲛鱼 Scomberomorus niphonius	4	0	00	0	00
马哈鱼 Oncorhynchus	8	0	00	0	00
虎头鱼 Sebastiscus marmoratus	9	0	00	0	00
沙丁鱼 Sardina pilchardus	12	0	00	0	00
舌鳎鱼 Cynoglossus robustus	8	0	00	0	00
马头鱼 Branchiostegus argentatus	5	0	00	0	00
鮸鱼 Miichthys miiuy	6	0	00	0	00
鲻鱼 Mugil cephalus	6	0	00	0	00
鲈鱼 Lateolabrax maculatus	5	0	00	0	00
合计	338	116	34.3	1 065	09.2

[1]	王陆儿, 郑玉华, 姬春花, 薛淑琴. 2022—2023年山西省高平市5例内脏利什曼病病例流行病学调查与处置[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(1): 143-146.
[2]	韩松, 雷霞, 聂莉, 罗文博, 姜晓峰. 内蒙古自治区棘球蚴病流行基线抽样调查与分析[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(1): 26-29.
[3]	王文雅, 刘剑峰, 张米禛, 钱沛君, 刘柳, 赵陆源, 李科荣, 张冬琦, 刘瑜华, 刘宏坤, 陈绍荣, 周长海, 钱门宝, 李石柱. 云南大理人群带绦虫感染和血清囊尾蚴抗体调查及其影响因素分析[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(5): 615-622.
[4]	李春燕, 张福艳, 史鹏, 田丰源. 2011—2023年四川省自贡市输入性疟疾疫情分析[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 129-133.
[5]	蔡长煌, 张芝平, 卓鸣莺, 郭理平, 傅志辉, 刘亦若. 基于虫卵内转录间隔区2序列分析诊断麝猫后睾吸虫和华支睾吸虫感染[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 427-433.
[6]	张娟, 陶洪, 李彦忠, 王婷婷, 杨晶晶, 向以斌, 陈奕杉, 周晓梅. 2017—2022年云南省常见螺类广州管圆线虫感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 464-469.
[7]	陈瑶, 张本光. 异尖属线虫分类学研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 480-485.
[8]	王峰, 吴凡, 李琳琳, 黄青青. 安徽省芜湖市野鼠寄生虫感染情况分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 516-519.
[9]	杜鹃, 李佳, 吴迪, 余琦, 张玮, 白如念, 郭俊林, 刘庆斌, 雷琪莉, 谷传慧, 王萌, 赵浩军. 2022年北京市犬猫刚地弓形虫感染血清流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 389-392.
[10]	郑丹, 林陈鑫, 蔡武卫, 谢汉国. 2019—2021年福建省海鱼异尖线虫幼虫感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 238-240.
[11]	郭占景, 张世勇, 刘立. 石家庄市内脏利什曼病复现流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 241-244.
[12]	刘建成, 许艳, 王龙江, 孔祥礼, 王用斌, 李曰进. 2015—2021年山东省临沂市输入性疟疾疫情监测分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 249-252.
[13]	李丹, 纪锋颖, 王龙江, 王用斌, 李曰进, 闫歌, 许艳, 卜秀芹, 张本光. 2021年青岛市海鱼异尖线虫感染及居民对异尖线虫病知晓情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(1): 52-58.
[14]	吴向林, 闫芳, 段红菊, 齐蓉婷, 马天波, 高建炜. 2021年宁夏多房棘球蚴病流行区犬和野外宿主感染情况[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 717-722.
[15]	杨成运, 贺志权, 鲁德领, 钱丹, 刘颖, 李素华, 周瑞敏, 邓艳, 张红卫, 王昊, 赵东阳, 郭万申. 2020年河南省内脏利什曼病病例的流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(4): 481-486.