安徽农村地区HIV感染者和幼儿园儿童蓝氏贾第鞭毛虫感染情况及其基因型

中国寄生虫学与寄生虫病杂志

安徽农村地区HIV感染者和幼儿园儿童蓝氏贾第鞭毛虫感染情况及其基因型

俞英昉，吴秀萍，储言红，陈家旭，田利光*

中国疾病预防控制中心寄生虫病预防控制所，世界卫生组织热带病合作中心，科技部国家级热带病国际联合中心，卫生部寄生虫病原与媒介生物学重点实验室，上海200025

出版日期:2016-12-30 发布日期:2017-01-10
基金资助:
国家自然科学基金（No. 81473022）和上海市卫计委科研课题（No. 140826120622123）

Infection of Giardia lamblia in HIV-Infected Individuals and in Kindergarden Children in Rural Area of Anhui and Genotype Analysis

YU Ying-fang, WU Xiu-ping，CHU Yan-hong, CHEN Jia-xu, TIAN Li-guang*

1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention；WHO Collaborating Centre for Tropical Diseases；National Center for International Research on Tropical Diseases, Ministry of Science and Technology；Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China

Online:2016-12-30 Published:2017-01-10
Supported by:
Supported by the National Natural Science Foundation of China（No. 81473022）and Shanghai Municipal Commission of Health and Family Planning Scientific Research Project（No. 140826120622123）

摘要/Abstract

摘要：

目的了解和比较安徽农村地区人类免疫缺陷病毒（human immunodeficiency virus，HIV）感染者和幼儿园儿童中蓝氏贾第鞭毛虫（Giardia lamblia）感染情况及其基因型。方法 2015年4月24日至5月9日收集安徽农村地区某艾滋病治疗点登记在册的HIV感染者及当地一家幼儿园所有儿童的相关信息，并采集粪样进行碘液染色及镜检。提取镜检阳性者的粪样DNA，采用巢式PCR扩增蓝氏贾第鞭毛虫磷酸丙糖异构酶（triosephosphate isomerase，tpi）基因并测序，通过BLAST、ClustalX 1.83和MEGA 6.0软件对基因序列进行同源性比较和系统发育分析。结果共调查HIV感染者127人和儿童125人，发现贾第鞭毛虫感染者4例，其中儿童3例，HIV感染者1例，感染率分别为 2.40%（3/125）和0.79%（1/127），两者差异无统计学意义（P>0.05）。3例儿童感染者粪便为成形软便，无腹泻、腹痛等临床症状；1例HIV感染者粪便为稀便，且有明显腹痛、腹泻、乏力症状，体重下降明显。PCR检测结果显示，4例感染者粪样DNA扩增产物均在约500 bp处出现特异条带。测序结果显示，4例均感染蓝氏贾第鞭毛虫，其中HIV感染者2个重复样品的序列相似性为79%，与已报道的上海人源贾第鞭毛虫分离株（GenBank登录号：KF271445）相似性分别为79%、98%；3名儿童感染者的样本序列相似性达99%，与上海人源贾第鞭毛虫分离株相似性均为79%。以tpi基因构建的系统进化树结果显示，HIV感染者的蓝氏贾第鞭毛虫分离株为复合型集聚体A+B，3名儿童感染者分离株均为集聚体A；HIV感染者的集聚体A与3名儿童感染者分离株的同源性较高。结论安徽农村地区HIV感染者和儿童人群中存在蓝氏贾第鞭毛虫感染，HIV感染者的虫株基因型为复合型集聚体A+B，儿童的虫株基因型皆为集聚体A。

关键词: 儿童, 人类免疫缺陷病毒感染者, 蓝氏贾第鞭毛虫, 分子特征

Abstract:

Objective To understand the situation of Giardia lamblia infection in HIV-infected individuals and in kindergarden children in rural area of Anhui Province and analyze the genotype of the parasite. Methods HIV-infected individuals registered in an AIDS treatment facility and children in a local kindergarden were included in this study during April 24 and May 9, 2015. The feces were collected, stained by iodine solution, and examined by microscopy. DNA was extracted from the positive feces, and nested PCR was performed to amplify the triosephosphate isomerase（tpi） gene of G. lamblia. The products were sequenced. The phylogenetic tree was constructed with BLAST, ClustalX 1.83 and MEGA6.0 softwares for analysis of homology and phylogeny. Results One hundred and twenty-seven HIV-infected individuals and 125 kindergarden children were included. G. lamblia infection was found in three children and one HIV-infected individual. The infection detection rate in children and HIV patients was 2.40% （3/125） and 0.79% （1/127）, respectively （P>0.05）. Feces of the three infected children was soft, and no symptoms of diarrhea and stomachache were complained. Feces of the HIV-infected individual was washy, and symptoms like diarrhea, stomachache, weakness and weight loss were reported. PCR produced a specific band at 500 bp for the four persons. The sequencing results further confirmed infection in these four persons. The duplicate samples of the infected HIV patient had a 79% sequence similarity, and were 79% and 98% homologous to the Shanghai human strain of G. lamblia（GenBank accession No: KF271445）, respectively. The samples of the 3 children had a 99% similarity, and all were 79% homologous to the Shanghai human strain of G. lamblia. The phylogenetic tree showed that the isolate from the HIV patient was mixed genotype of A+B, while those from the 3 children were all assemblage A. There was a high similarity between the isolates. Conclusions There is Giardia infections in HIV patients and kindergarden children in the area. The genotype of the isolate from the HIV individual is mixed assemblage A+B while those from the children are assemblage A.

Key words: Children, HIV-infected individual, Giardia lamblia, Molecular characteristic

俞英昉，吴秀萍，储言红，陈家旭，田利光*. 安徽农村地区HIV感染者和幼儿园儿童蓝氏贾第鞭毛虫感染情况及其基因型[J]. 中国寄生虫学与寄生虫病杂志.

YU Ying-fang, WU Xiu-ping，CHU Yan-hong, CHEN Jia-xu, TIAN Li-guang*. Infection of Giardia lamblia in HIV-Infected Individuals and in Kindergarden Children in Rural Area of Anhui and Genotype Analysis[J]. Chinese Journal of Parasitology and Parasitic Diseases.

[1]	朱爱娅, 王旭, 王江友, 王颖, 李杨, 宋珊, 耿燕, 兰子尧, 戴佳芮. 贵州省儿童多房棘球蚴病1例[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 520-523.
[2]	李芳, 王淑芳, 贺朋飞, 徐淑慧. 土源性线虫感染对农村学龄前儿童喘息、哮喘和皮肤点刺试验阳性的影响[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 336-343.
[3]	王丹, 贺志权, 刘颖, 刘灵芝, 陈慧慧, 蒋甜甜, 纪鹏慧, 钱丹, 杨成运, 张红卫. 1例蓝氏贾第鞭毛虫感染者的虫种分子鉴定及基因溯源[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 380-383.
[4]	路伟民, 杨小涛, 朱瑛, 张鸿, 李霁伟, 王艳春. 儿童肺细粒棘球蚴病1例[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 253-256.
[5]	潘火云, 谭丽梅, 徐翼, 李旭芳, 叶家卫, 房春晓, 杨峰霞, 陈敏霞, 杨花梅, 曾凡森. 12例儿童广州管圆线虫病临床分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 668-672.
[6]	朱名超, 朱娅. HIV合并人芽囊原虫和蓝氏贾第鞭毛虫感染1例[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(3): 315-318.
[7]	易佳, 张华勋, 夏菁, 张聪, 朱红, 曹慕民, 何汇, 张娟. 2018—2020年湖北省儿童蛲虫感染监测结果分析[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(5): 606-610.
[8]	李淑凝, 李汶霖, 沈海娥, 王洋, 田喜凤. 蓝氏贾第鞭毛虫滋养体体外诱导中性粒细胞胞外诱捕网的形成[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(4): 455-460.
[9]	黎敏, 骆清清, 房辉, 曹晓琴. 花椒乙醇提取物体外抗蓝氏贾第鞭毛虫的作用[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(3): 359-364.
[10]	于铭川, 杨忠委, 王华然, 师丹阳, 周树青, 尹静, 孙树民. 基于LAMP微流控芯片检测蓝氏贾第鞭毛虫方法的建立[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(3): 402-405.
[11]	陈喆, 姜唯声, 葛军, 戴坤教, 李东, 刘克星, 曾小军. 2016-2019年江西省农村地区儿童蛲虫监测结果分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(5): 542-547.
[12]	谢贤良, 谢汉国, 江典伟, 陈云虹, 陈宝建. 福建省第三次儿童蛲虫感染调查分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(5): 607-610.
[13]	许薇, 朱志伟, 罗欣, 孙恩涛, 赵金红. 腐食酪螨的形态和分子特征鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1): 95-101.
[14]	袁义雪, 郑国侠, 王丹丹, 专行, 杜萌, 王云华. 蓝氏贾第鞭毛虫核苷二磷酸激酶基因的克隆与表达[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(2): 190-193.
[15]	龚志红, 徐芸, 刘克星, 戴坤教, 曾小军, 宁安, 谢慧群, 祁爱民, 黄成坚, 涂永红, 徐晶, 高祖禄, 姜唯声. 江西省本地儿童肺细粒棘球蚴感染1例报告分析[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(2): 228-231.