中国寄生虫学与寄生虫病杂志 ›› 2008, Vol. 26 ›› Issue (5): 18-396.

• 研究简报 • 上一篇    下一篇

3 种常见尘螨分子进化关系的初步探讨

崔玉宝1,2*,高璀乡1,周鹰1,彭江龙2,刘良1   

  1. 1 盐城卫生职业技术学院病原生物学教研室,盐城 224006;2 海南医学院海南省热带病原学重点实验室,海口571101
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-10-31 发布日期:2008-10-31
  • 通讯作者: 崔玉宝

Preliminary Investigation on Phylogenetic Relationship AmongThree Common Species of House Dust Mites

CUI Yu-bao1,2*,GAO Cui-xiang1,ZHOU Ying1,PENG Jiang-long2,LIU Liang1   

  1. 1 Department of Pathogenic Biology,Yancheng Health Vocational & Technical College,Yanchen 224006,China; 2 Hainan Provincial Key Laboratory of Tropical Etiology,Hainan Medical College,Haikou 571101,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-31 Published:2008-10-31
  • Contact: CUI Yu-bao

摘要:

为探讨粉尘螨(Dermatophagoides farinae)、屋尘螨(Dermatophagoides pteronyssinus)和梅氏嗜霉螨(Euroglyphus maynei)的分类,采用PCR扩增获得粉尘螨1类(Der f 1)和2类变应原(Der f 2)的cDNA片段,经测序后推导氨基酸序列, 与GenBank中的屋尘螨Der p 1、Der p 2和梅氏嗜霉螨Eur m 1、Eur m 2氨基酸序列进行比对,分别构建1类和2类变应原分子进化树。结果显示变应原之间相似度,Der p 1与Eur m 1为86%,Der p 1与Der f 1为83%;Der p 2与Eur m 2为87%,Der p 2与Der f 2为67%。用变应原氨基酸序列构建分子进化树,1类变应原Der p 1与Eur m 1聚成一簇,2类变应原Der p 2与Eur m 2聚成一簇。结果表明屋尘螨和梅氏嗜霉螨亲缘关系较近,而与粉尘螨较远。

关键词: 粉尘螨, 屋尘螨, 梅氏嗜霉螨, 分类, 分子进化

Abstract: Dermatophagoides farinae,D. pteronyssinus and Euroglyphus maynei were used for the investigation. The cDNA fragment coding for Der f 1 and Der f 2 were amplified by PCR,cloned and sequenced. By bioinformatics softwares,the amino acid sequences for Der f 1 and Der f 2 were deduced and compared with those for the groups 1 and 2 allergens of D. pteronyssinus and E. maynei available in GenBank. Amino acid sequence similarity analysis showed that Der p 1 shared 84% identical residues with Eur m 1 and 83% with Der f 1. Similarly,Der p 2 shared 87% identical residues with Eur m 2 and 68% with Der f 2. In the two phylogenetic trees constructed with group 1 and 2 allergens,D. pteronyssinus was clustered with E. maynei but not with D. farinae,although D. pteronyssinus and D. farinae belong to the same genus. D. pteronyssinus should be more similar to E. maynei than to D. farinae at evolutional level,which was not consistent with the conventional taxonomical relationship based on their morphological characteristics.

Key words: Dermatophagoides farinae, Dermatophagoides pteronyssinus, Euroglyphus maynei, Taxonomy, Molecular evolution