线粒体基因在带绦虫科分子分类鉴定及系统进化研究中的应用

doi:10.12140/j.issn.1000-7423.2022.06.012

中国寄生虫学与寄生虫病杂志 ›› 2022, Vol. 40 ›› Issue (6): 767-773.doi: 10.12140/j.issn.1000-7423.2022.06.012

线粒体基因在带绦虫科分子分类鉴定及系统进化研究中的应用

陈齐鲁(), 王旭, 李春阳, 官亚宜^*()

中国疾病预防控制中心寄生虫病预防控制所（国家热带病研究中心），国家卫生健康委员会寄生虫病原与媒介生物学重点实验室，世界卫生组织热带病合作中心，国家级热带病国际联合研究中心，上海 200025

收稿日期:2022-04-09 修回日期:2022-08-08 出版日期:2022-12-30 发布日期:2022-12-16
通讯作者: 官亚宜
作者简介:陈齐鲁（1996-），男，硕士研究生，从事寄生虫病流行病学相关研究。E-mail：chen_qilu1@163.com
基金资助:
上海市卫生健康委员会科研项目(20214Y0231)

Application of mitochondrial genes in the molecular taxonomic identification and phylogenetic analysis of Taeniidae

CHEN Qi-lu(), WANG Xu, LI Chun-yang, GUAN Ya-yi^*()

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research); NHC Key Laboratory of Parasite and Vector Biology; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Shanghai 200025, China

Received:2022-04-09 Revised:2022-08-08 Online:2022-12-30 Published:2022-12-16
Contact: GUAN Ya-yi
Supported by:
Shanghai Municipal Health Commission Research Project(20214Y0231)

摘要/Abstract

摘要：

带绦虫科部分虫种所引起的棘球蚴病或囊尾蚴病是人兽共患寄生虫病，对社会经济和公共卫生造成重大影响。对相关虫种进行准确的鉴别有助于有效预防、控制甚至消除由其所致的疾病。目前在带绦虫科分子分类及系统进化研究中应用的线粒体基因主要为细胞色素C氧化酶亚基1、细胞色素B和NADH脱氢酶亚基1等基因。本文主要综述线粒体基因在带绦虫科中的带绦虫属和棘球绦虫属分子分类与系统进化方面的研究进展。

关键词: 带绦虫科, 线粒体基因, 分子分类鉴定, 系统进化

Abstract:

Echinococcosis or cysticercosis caused by the Taeniidae species is a zoonotic parasitic disease that has a significant impact on the social economy and public health. Accurate identification of the relevant species is helpful for the prevention, control, and even eliminate of the diseases. Currently, mitochondrial genes used in molecular classification and phylogenetic studies of Taeniidae are mainly cytochrome C oxidase subunit 1 gene, cytochrome B gene, and NADH dehydrogenase subunit 1 gene. This paper reviews the current advances of molecular classification and phylogenetic analysis of the Taenia and Echinococcus in the family Taeniidae, mainly from the perspective of mitochondrial genes.

Key words: Taeniidae, Mitochondrial gene, Molecular taxonomic identification, Phylogeny

中图分类号:

R383.2

陈齐鲁, 王旭, 李春阳, 官亚宜. 线粒体基因在带绦虫科分子分类鉴定及系统进化研究中的应用[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 767-773.

CHEN Qi-lu, WANG Xu, LI Chun-yang, GUAN Ya-yi. Application of mitochondrial genes in the molecular taxonomic identification and phylogenetic analysis of Taeniidae[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2022, 40(6): 767-773.

图/表 1

参考文献 60

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基因	优缺点	参考文献
cox1	较其他线粒体基因具有更有效的遗传信息，基因保守，适用于种间及种以上的分类鉴别和系统进化研究	[6,11⇓⇓-14]
cytb	进化速率适中，较短片段即包含种、属、纲水平的系统发育信息，序列相对稳定且高度保守，但种间的序列差异非常大，可作为牛带绦虫和亚洲带绦虫分类研究的分子标志	[24,57]
nad1	结构简单、进化速率快，较cox1基因更加适合作为研究绦虫系统进化的分子标志	[9,34⇓⇓-37]
其他线粒体基因	12S rRNA的高变异区位于保守延伸区内，多用于近缘种研究。nad5在带绦虫属中差异程度最大，在棘球绦虫属中变异率最大	[6,9,24,55]

线粒体基因在带绦虫科分子分类鉴定及系统进化研究中的应用

Application of mitochondrial genes in the molecular taxonomic identification and phylogenetic analysis of Taeniidae

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