Evaluation of up-converting phosphor-lateral flow method for rapid detection of IgG antibodies in echinococcosis patients

doi:10.12140/j.issn.1000-7423.2022.01.019

Abstract

Abstract:

To evaluate the effect of up-converting phosphor-lateral flow (UPT-LF) method for rapid detection of IgG antibodies in echinococcosis patient, and compare the test results with that of enzyme-linked immunosorbent assay (ELISA) method. Serum samples from patients with clinically confirmed echinococcosis were collected at Qinghai Provincial People’s Hospital and the Affiliated Hospital of Qinghai University from 2017 to 2018. Serum samples of healthy individuals who came for general healthy checks were also collected from the physical examination centers of those hospitals. The anti-echinococcosis IgG antibody in the samples was detected by UPT-LF and ELISA methods. The sensitivity, specificity, misdiagnosis rate, total coincidence rate and Yoden index were analyzed based on clinical diagnosis results. Chi-square test was performed to compare the results between the two methods. A total of 104 serum samples of echinococcosis patients, including 46 cases of alveolaris echinococcosis (AE), 58 cases of cyst echinococcosis (CE) and 165 serum samples from healthy individuals were collected. The sensitivity of UPT-LF and ELISA in echinococcosis patients were 94.2% (98/104) and 78.8% (82/104), respectively, with statistically significant difference between the two methods (χ² = 11.250, P < 0.01). The sensitivity of UPT-LF and ELISA in AE patients were 97.8% (45/46) and 89.1% (41/46), while 91.4% (53/58) and 70.7% (41/58) in CE patients, respectively. The specificity of UPT-LF and ELISA in non-echinococcosis patients were 95.2% (157/165) and 90.9% (150/165), respectively, with no statistically significant difference between the two methods (χ ² = 1.565, P > 0.05). Compared with the clinical diagnosis result, the coincidence rates of UPT-LF and ELISA were 94.8% (255/269) and 86.2% (232/269), respectively, with no statistically significant difference between the two methods (χ ² = 1.884, P > 0.05). The Youden index of UPT-LF and ELISA were 0.894 and 0.697, respectively. UPT-LF method showed higher sensitivity than that of ELISA, and has a good application prospect in the field for the rapid detection of echinococcosis.

Key words: Echinococcosis, Up-converting phosphor-lateral flow, Rapid detection

CLC Number:

R532.32

LIU Jia, MA Jun-ying, ZHANG Xue-fei, WANG Hu, MA Xiao, CAI Hui-xia, GAO Qi, QIE Shuang, YIN Yan, WANG Wei, WANG Yong-shun, ZHANG Jing-xiao, LIU Yu-fang, LEI Wen, ZHAN Pei-zhen, ZHANG Qing. Evaluation of up-converting phosphor-lateral flow method for rapid detection of IgG antibodies in echinococcosis patients[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(1): 121-123.

References 14

[1]	Eckert J. “Echincoccosis in humans: clinical aspects, diagnosis and treatment” in manual on echincoccosis in humans and animals: a public health problem of global concern[R]. WHO/OIE, 2001, 20-145.
[2]	Chen R, Wang ZX, Zhou LX, et al. Progress of researches on novel diagnostic markers for alveolar echinococcosis[J]. Chin J Schisto Control, 2021, 33(4): 1-5. (in Chinese)
	(陈锐, 王志鑫, 周留馨, 等. 多房棘球蚴病新型诊断标志物研究进展[J]. 中国血吸虫病防治杂志, 2021, 33(4): 1-5.)
[3]	Aimaiti YS, Wen H. Application of immunology in the study of echincoccosis[J]. J Xinjiang Med Univ, 2020, 43(6): 830-836. (in Chinese)
	(艾麦提·牙森, 温浩. 免疫学在包虫病研究中的应用[J]. 新疆医科大学学报, 2020, 43(6): 830-836.)
[4]	Huang Y, Shang JH, Chen F, et al. Serological detection of echincoccosis and its diagnostic significance[J]. Chin Animal Health, 2017, 19(7): 22-25. (in Chinese)
	(黄燕, 尚婧晔, 陈凡, 等. 包虫病血清学检测方法及其诊断意义[J]. 中国动物保健, 2017, 19(7): 22-25.)
[5]	Hao QF, Zhang LP, Yang XL, et al. Evaluation of the development of syphilis IgG antibody detection reagent based on an up-converting phosphor-lateral flow technique[J]. Chin J Health Lab Technol, 2015, 25(19): 3399-3402. (in Chinese)
	(郝钦芳, 张丽萍, 杨晓莉, 等. 基于上转发光技术研发梅毒IgG抗体检测试剂的评价[J]. 中国卫生检验杂志, 2015, 25(19): 3399-3402.)
[6]	Zhou L. The development of UPT-based biosensor[D]. Beijing: Chinese People’s Liberation Army Academy of Military Medical Sciences, 2004: 70-74. (in Chinese)
	(周蕾. 基于上转换发光技术生物传感器的研制[D]. 北京: 中国人民解放军军事医学科学院, 2004: 70-74.)
[7]	Lv C, Qing ZQ, Xu J. Use of up-converting phosphor technology-based lateral flow assay in biomedical detection[J]. Chin J Pathogen Biol, 2017, 12(12): 1231-1235. (in Chinese)
	(吕超, 秦志强, 许静. 上转发光层析技术及其在生物医学检测领域中的应用进展[J]. 中国病原生物学杂志, 2017, 12(12): 1231-1235.)
[8]	Li YL, Zhang LP, Zhang DH, et al. Develoment and devaluation of up-converting phosphor technology to detect syphilis cardiolipin antibody[J], Chin J AIDS STD, 2018, 24(3): 275-278. (in Chinese)
	(李迎丽, 张丽萍, 张丹浩, 等. 上转发光免疫层析技术检测梅毒心磷脂抗体的研制及初步评价[J]. 中国艾滋病性病, 2018, 24(3): 275-278.)
[9]	Wang MC. The clinical significance of up-converting phosphor immunochromatography in detecting hepatitis B virus’ large envelope protein in various types of chronic liver disease[D]. Hangzhou: Zhejiang university, 2015: 29-32. (in Chinese)
	(王妙婵. 上转发光免疫层析法检测乙肝病毒外膜大蛋白在不同类型慢性肝病中的临床意义[D]. 杭州: 浙江大学, 2015: 29-32.)
[10]	Hao M. Develoment of a UPT-based Immunochromatogrraphic assay for detection of Vibrio cholerae[D]. Beijing: Chinese Center for Disease Control and Prevention, 2010: 59-61. (in Chinese)
	(郝民. 应用基于上转换发光技术的免疫层析法检测霍乱弧菌[D]. 北京: 中国疾病预防控制中心, 2010: 59-61.)
[11]	Zhao J. Develoment of UPT immunochromatogrraphic strip for rapid detection of brucella antigen[D]. Beijing: Chinese People’s Liberation Army Academy of Military Medical Sciences, 2009: 58-60. (in Chinese)
	(赵瑾. 布鲁氏菌抗原快速检测UPT免疫层析试纸的研制[D]. 北京: 中国人民解放军军事医学科学院, 2009: 58-60.)
[12]	Wang C, Zhang PP, Zhao Y, et al. Establishment of an up-converting phosphor-lateral flio (UPT-LF) method for rapid detection of anti Trichinrlla IgG antibodies in swine[J]. Chin J Vet Med, 2019, 39(5): 931-935. (in Chinese)
	(王春, 张平平, 赵勇, 等. 上转发光免疫层析技术快速检测猪抗旋毛虫IgG抗体方法的建立[J]. 中国兽医学报, 2019, 39(5): 931-935.)
[13]	Wang Y, Zhang J, Yang ZY, et al. Cloning, expression and immunodiagnostic evaluation of enolase from Echinococcus granulosus[J]. Chin J Schisto Control, 2012, 24(5): 549-552, 556. (in Chinese)
	(王莹, 张璟, 袁忠英, 细粒棘球绦虫烯醇酶基因克隆表达及免疫诊断研究[J]. 中国血吸虫病防治杂志, 2012, 24(5): 549-552+556.)
[14]	Zhong HY, Yang H, Shu L, et al. Application evaluation of detecting serum gydatid IgG antibody with ELISA method[J], Parasit Infect Dis, 2014, 12(1): 18-20. (in Chinese)
	(钟洪燕, 杨浩, 舒玲, 等. ELISA法检测血清包虫IgG抗体试剂盒的应用评价[J]. 寄生虫病与感染性疾病, 2014, 12(1): 18-20.)

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