Table of Content

31 December 2010, Volume 28 Issue 6

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Cloning, Expression of PPMP Antigen Genes of Clonorchis sinensis and Immunogenic Identification of the Recombinant Proteins

XU Xue-nian，ZHOU Yan，DONG Yu-ting，TAN Yu-guang，BAO Yi-fang，XU Bin1，CHENG Na，XU Hong-bo，LI Xue-ming，FENG Zheng

2010, 28(6):  1-401-405. 

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【Abstract】  Objective   To screen and identify new specific antigen gene from a cDNA library of adult Clonorchis sinensis, and investigate the immunogenicity of the recombinant proteins.   Methods   The λZAP cDNA library of adult C. sinensis was immunoscreened with pooled sera of clonorchiasis patients. The positive clones were sequenced and analyzed. The sequence encoding the mature peptide was cloned into prokaryotic expression vector pET28b（+）. The recombinant plasmid was transformed into E. coli BLR21 （DE3） or BLR21 （DE3） pLysS and followed by expression of the protein induced by IPTG. The recombinant protein was purified by His-bind-resin （Ni-NTA） affinity chromatography and identified by Western blotting. BALB/c mice were immunized with purified recombinant pET28b-Cs2 protein, and the sera from immunized mice were analyzed for specific antibodies by ELISA.  Results   A total of 44 positive clones were isolated from the C. sinensis cDNA library. Three clones containing specific tandem repeats of PPMP amino acid sequence were named as C. sinensis PPMP antigen genes. The genes containing KPPMPGDRDA, QPPMPGGRDA were named as type PPMPⅠ and type PPMPⅡ anti-gens, respectively. Sequence analysis revealed that these PPMP genes were a novel specific C. sinensis antigen gene family. Two new genes, PPMPⅠ Cs2 and PPMPⅡ Cs3, were expressed in E. coli, and SDS-PAGE showed that the two recombinant proteins were about Mr 22 000 and Mr 39 000. The two soluble recombinant proteins were recognized by pooled sera of clonorchiasis patients. A high level of specific IgG against the recombinant proteins （maximum dilution 1 ∶ 64 000） was produced in immunized mice.   Conclusion   A novel PPMP gene family of C. sinensis has been identified, and its re-combinant proteins show high immunogenicity.

Survey on the Foci of Paragonimus in Youxi， Yongtai and Pinghe Counties of Fujian Province

CHENG You-zhu，LI Li-sha，LIN Guo-hua，ZHOU Pei-cong，JIANG Dian-wei，FANG Yan-yan，LIN Chen-xin，LI Yan-rong

2010, 28(6):  2-406-410. 

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【Abstract】   Objective   To investigate the natural foci of paragonimiasis in Youxi, Yongtai and Pinghe Counties of Fujian Province.  Methods   One village each from the three counties was selected according to reported para-gonimiasis cases. Freshwater snails collected from fields were examined for cercariae. Freshwater crabs obtained in the fields were examined for metacercariae by washing filtration method or direct compression method. Feces of cats and dogs were collected for the detection of eggs by water precipitation. In order to identify the species of Paragonimus, 4 cats were orally fed with metacercariae. At the same time, the habitat of three survey sites was observed.   Results   Three fluke species, namely, P. skrjabini, P. westermani and P. cenocopiosus（Syn. Euparagonimus cenocopiosus） were found. In Banlin Village of Youxi County and Chishui Village of Yongtai County, the seropositive rate by IgG ELISA were 6.8% （21/309） and 6.8% （9/133）, respectively. Four species of freshwater snails were found, two species of Tricula and one species of Pseudo-bythinella were newly identified  first intermediate hosts of Paragonimus. Four species of freshwater carbs were found, one species of Nanhaipotamon served as a new second intermediate host of Paragonimus. In Youxi, the infection rate of P. skrjabini cercariae in snails and metacercariae in crabs was 2.1% （27/1 344） and 92.1% （58/63）, respectively; the index of crab infection and the positive ratio of Paragonimus eggs in cat feces was 171.91 and 1/7, respectively. In Yongtai, the cercariae infection rate in Pseudobythinella and Tricula snails infected with P. skrjabini was 0.6%（4/690） and 0.1% （2/2 330）, respectively; the infection rate of P. skrjabini metacercariae in crabs was 18.2% （18/99）; the index of crab infection was 9.12. In Pinghe, the infection rate of cercariae in Semisulcospira libertina and metacercariae in crabs was 0.3% （3/1 092） and  44.9% （35/78）, respectively; the index of crab infection was 6.16.  Conclusion   Paragonimus skrjabini, P. westermani and P. cenocopiosus have been found from the three counties of Fujian Province with different infection level in snails and crabs.

Kinetics of IgA Secreting Cells and sIgA in Small Intestine of MiceInduced by Intranasal Immunization with Toxoplasma gondii STAg

SHEN Jin-yan，YIN Guo-rong，LIU Hong-li，YIN Li-tian，MENG Xiao-li，WANG Hai-long

2010, 28(6):  3-411-415. 

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【Abstract】   Objective   To investigate the kinetics of IgA secreting cells （IgASCs） and secretory IgA （sIgA） level in small intestine induced by intranasal immunization with Toxoplasma gondii soluble tachyzoite antigen （STAg） in mice.  Methods   Ninty?鄄six 5 to 6?鄄week old BALB/c mice were randomly divided into immunity and control groups. Mice of the immunity group were each intranasally immunized with STAg 20 μg in 20 μl PBS， twice at an interval of 2 weeks， while the control mice were each given 20 μl PBS. All mice were challenged intragastrically with 1×104 tachyzoites in 0.5 ml per mouse in 1 week after the last immunization. The body weight and infection incidence of mice were recorded. Eight mice of each group were sacrificed on the day 6， 7， 8， 9， 10 and 11 post infection， respectively. The quantity of IgASCs in mucosa of duodenum， jejunum and ileum was detected by immunohistochemistry. The sIgA in intestinal washes were determined by ELISA.  Results   All mice fell ill post infection， but the symptom of mice in the immunity group was milder, the increasing level of body weight of mice in the immunity group was higher considerably than that in the control group（P＜0.05）. Two mice died in control group on the 7th day after infection. sIgA level in intestinal washes increased continually in two groups, but the increasing level in the immunity group was higher than that of the control （P＜0.05）. The number of IgASCs in duodenum increased slightly in the control group, but increased continuously and maintained a high level after 9 d in the immunity group， for instance， 20.65±1.67 in the immunity group and 12.30±2.67 in the control. The correlation of the sIgA level in intestinal washes and the quantitative change of IgASCs in duodenum was positive in the immunity group（r=0.566, P＜0.05） and the control（r=0.378, P＜0.05）. The number of IgASCs in jejunum decreased in the control group but increased then slightly decreased after 9 d in the immunity group. Positive correlation between the sIgA level in intestinal washes and the quantitative change of IgASCs in jejunum was found in the immunity group（r=0.218, P＞0.05） but negative in the control（r=-0.557, P＜0.05）. The number of IgASCs in ileum declined in the control group but maintained a high level in the immunity group. The correlation bewteen the sIgA level in intestinal washes and the quantitative change of IgASCs in ileum was  r=-0.053（P＞0.05）in the immunity group and r=-0.685（P＜0.05）in the control.   Conclusion   Intranasal immunization with STAg in mice orally infected with Toxoplasma gondii can increase the number of IgASCs in jejunum and ileum, and enhance the immune barrier function of mucosa in small intestine of mice.

In Vitro Anti-Trichomonas vaginalis Effects of a Mixture of Dihydroartemisinin and Metronidazole

TANG Zi-hao，LIU Ke-yue，MEI Jun，GAO Xing-zheng

2010, 28(6):  4-416-422. 

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【Abstract】  Objective   To observe the effect of a mixture of dihydroartemisinin and metronidazole on ultrastructure of Trichomonas vaginalis trophozoites in vitro for exploring trichomonacidal mechanism of the drug mixture.   Methods  The trophozoites were cultivated with liver extract solution medium that contained 2.5×106 parasites/ml. There were dihydroartemisinin 0.5 mg/ml and metronidazole 0.002 mg/ml in the experimental tubes of the drug mixture group. Groups of control （without drug）， dihydroartemisinin （1 mg/ml） and metronidazole （5 mg/ml） were established and performed in the same experimental conditions. The parasites were observed by scanning and transmission electron microscopes after having treated with the drugs at 37 ℃ for 3.5-5 h. Results Under scanning electron microscope，the cell membrane of T. vaginalis treated only with dihydroartemisinin for 3.5 h was damaged，part of pellicle peeled off. Although the surface of the trophozoites treated only with metronidazole for 5 h showed many small bubbles and hollows，the cell membrane looked integral. However，surface of the parasite exposed to the drug mixture for 3.5-4.2 h showed deep folds and cracks，the cell membrane was damaged and even peeled off. When the cell ruptured，the nucleus， axostyle， pelta and hydrogenosomes were exposed，and the cytoplasm spilled out. Transmission electron microscopy showed that the membrane system of the trophozoites treated only with dihydroartemisinin for 3.5 h was damaged considerably. The cytoplasm of damaged parasite spilled out. The cytoplasm of the parasite treated only with metronidazole for 3.5-5 h was damaged seriously. Vacuoles and crevices were visible in the cytoplasm. The cell membrane and the content of the parasites treated with the drug mixture for 3.5-4.5 h were damaged seriously. There were some vacuoles and crevices， dilated endoplasmic reticulum， injured and deformed hydrogenosomes in the cytoplasm. The cell organelles mostly disap-peared. Crevices also existed in the nucleus. The nuclear membrane fractured and even disappeared.  Conclusion The acting targets of dihydroartemisinin and metronidazole to T. vaginalis trophozoite were different，and a combination of the two drugs shows stronger effect in killing the parasites.

Intelligence Level and Characteristics of Cognitive Structure in School-age Children Infected with Soil-transmitted Helminthes

SHANG Yu，TANG Lin-hua

2010, 28(6):  5-423-426. 

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【Abstract】   Objective   To investigate the intelligence level and characteristics of cognitive structure of school-age children infected with soil-transmitted helminthes （STH）（hookworm, Ascaris lumbricoides and Trichuris trichiura）.   Methods  1 031 children of 9 to 12 years old from Baishui village of Guangxi, Longzhou and Xinzhu villages of Hainan were selected for the survey. Stool samples from children were collected and examined by modified Kato-Katz method. 77 children infected with only one species （hookworm,  A. lumbricoides, or T. trichiura） were used as case group and 39 healthy children as control. The cognitive ability for children were assessed by WISC-Ⅳ.   Results   A total of 378 cases with STH infection was found from 1 031 children, and the overall prevalence of STH infection was 36.7%. The prevalence of moderate and heavy STH infections was 16.8% （173/1 031）. The verbal comprehension （VCI）, working memory （WMI）, processing speed （PSI） and full scale IQ （FSIQ） indices of case group were lower than those of control group （P＜0.05）. The VCI （97.2）, WMI （84.6）, PSI （91.1） and FSIQ （95.4） indices in moderate/heavy infection group were lower than those in light infection group （99.2，103.3，88.3 and 99.1, respectively） and control group （102.0，104.2，91.9 and 100.1,  respectively）（P＜0.05）. The WMI index in hookworm infection group （83.7） was lower than that in A. lum-bricoides （87.6） or T. trichiura （88.3） infection groups （P＜0.05）. The PSI index of T. trichiura infection group （92.8） was lower than that of A. lumbricoides （97.3） or hookworm （94.4） infection groups （P＜0.05）.   Conclusion   The total intelligence level of STH infection children is in a normal range, or on borderline. The low VCI, WMI, PSI indices have been found in moderate/heavy STH infection children.

Effect of Nutritional Stress on Autophagy in Free-Living Amoeba

WANG Nan-ning，TAN Yu-zhen，WANG Hai-jie

2010, 28(6):  6-427-430. 

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【Abstract】  Objective   To investigate the change of autophagy and morphological characteristics of the autophagic structures in free-living amoeba under nutritional stress.  Methods   Free-living amoebae were incubated on the agaric solid medium which had been covered with Escherichia coli in control group. In the experiment group， amoebae incubated on the agaric solid medium with E. coli were collected and moved to another solid medium without E. coli and incubated for 12 h. The morphological changes of free-living amoeba in the medium without E. coli were viewed with scanning electron microscope. The changes of autophagy and the structural features of the autophagosome precursors， autophagosomes and autophagolysosomes in amoeba were examined with transmission electron microscope， and the cross-section areas of the autophagic structures and cytoplasm were measured with an image analyzer. The autophagosomes in the organism were labeled with monodansylcadaverine （MDC） staining and quantitated using laser scanning confocal microscope.   Results   In the control group， free-living amoebae were all in the form of trophozoite. In the experiment group， trophozoites were induced to transform to cysts gradually. In control group， amoeba was full of fragment of E. coli. There was merely little autophagy with fewer autophagic structures in amoeba. When compared with the control group， the autophagic abilities of amoeba were enhanced significantly， number of autophagic structures increased in the experiment group. In addition， the ratio of the cross-sectional areas of the autophagic structures to that of the cytoplasm of amoeba was greater （P＜0.05 or 0.01）. There was fragment of E. coli that was not digested in some of the amoebae.  Conclusion   In the circumstance of nutritional stress， amoebic trophozoites were induced to transform to cysts gradually. The autophagic ability of free-living amoeba significantly enhanced.

MGE-PCR in Analyzing the Genetic Characteristics and the Clinical Manifestation of Trichomonas vaginalis Isolates

WEN Wen-jing，LIU Xiao-dong

2010, 28(6):  7-431-434. 

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【Abstract】   Objective   To analyze the genetic characteristics of Trichomonas vaginalis isolates causing different clinical manifestation.  Method   Mobile genetic factor （MGE）-PCR technique was used for genome amplification in 30 isolates of Trichomonas vaginalis with different clinical manifestation （mild 3, moderate 22, severe 5），and neighbor joining method （NJ） was applied for cluster analysis of the amplified products.  Results   Significant genetic differences were revealed among the isolates，with a distance ranging from 0 to 1 and an average genetic distance of 0.69. A dendrogram based on the genetic difference among the isolates formed 3 clusters. Those with severe or mild clinical manifestation located in cluster 1 (Tv10，Tv15，Tv13，Tv21，and Tv25) or cluster 2 (Tv4，Tv14，and Tv26)，respectively. The other 18 isolates，except Tv2，Tv5，Tv17，and Tv28，all distributed in the cluster 3 at the top of the large branches of the tree. Close genetic relationship was found in 5 groups with a genetic distance of 0：Tv12 and Tv29，Tv18 and Tv20，Tv14 and Tv26，Tv10 and Tv25，Tv15 and Tv21.   Conclusion   The clustering of the 30 T. vaginalis isolates is related to the degree of clinical manifestation that they caused，indicating a possible relationship between morbidity and the gene difference of the parasite.

Investigation on Contamination of Cryptosporidium and Giardia in Drinking Water and Environmental Water in Shanghai

ZHANG Xiao-ping，HE Yan-yan，ZHU Qian，MA Xiao-jiang，CAI Li

2010, 28(6):  8-435-438. 

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【Abstract】   Objective   To understand the contamination status of Cryptosporidium sp. and Giardia lamblia in drinking water, source water and environmental water in Shanghai.  Methods   All water samples collected from drinking water, source water and environmental water were detected by a procedure of micromembrane filtration, immune magnetic separation（IMS）, and immunofluorescent assay（IFA）.   Results   Cryptosporidium oocysts and Giardia cysts were not found in 156 samples of the drinking water including finished water, tap water, or pipe water for directly drinking in communities. Among 70 samples either source water of water plants（15 samples）, environmental water from Huangpu River（25）, canal water around animal sheds（15）, exit water from waste-water treatment plants（9）, or waste water due to daily life（6）, Cryptosporidium oocysts were detected in 1（6.7%）, 2（8.0%）, 7（46.7%）, 1（11.1%）, and 1（16.7%） samples, respectively; and Giardia cysts were detected in 1（6.7%）, 3（12.0%）, 6 （40.0%）, 2（22.2%）, and 2（33.3%）, respectively. The positive rate of  Cryptosporidium oocysts and Giardia cysts was 17.1%（12/70） and 20.0%（14/70）, respectively.   Conclusion   No Cryptosporidium oocysts and Giardia cysts have been detected in drinking water, but found in source water and environmental water samples in Shanghai.

Comparative Evaluation of Five Test Kits for Antibody Detection in Schistosoma japonicum Endemic Areas of Poyang Lake Region

LIN Dan-dan，XU Jing，LIU Hong-yun，ZENG Xiao-jun，LIU Yue-min，XIE Shu-ying，HU Fei，LI Jian-ying，HUANG Mei-jiao，CHEN Hong-gen，ZHOU Xiao-nong

2010, 28(6):  9-439-443. 

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【Abstract】  Objective   To evaluate the efficacy of five diagnostic test kits for the detection of antibodies to  Schistosoma japonicum in endemic areas of Poyang Lake.  Methods   Residents over 5 years old in three schistosomiasis-endemic administrative villages in the lake region were simultaneously examined by modified Kato-Katz thick smears and nylon bag sedimentation/hatching method. At the same time, five sero-diagnostic test kits for detection of IgG antibody against soluble egg antigen of S. japonicum were carried out.  Results  The distribution of seropositive rates of S. japonicum IgG antibodies determined by sero-diagnostic tests showed similar trends with that of egg positive rates in different age groups, but the seropositive rate （33.1%-57.1%） was significantly higher than that of egg positive rate （5.0%-8.2%）, both reached a peak in 40-49-year-old group. The antibody positive rates determined by each test increased with the infection intensity. The sensitivity of the evaluated test kits ranged from 81.2%-94.5%. 26.9% to 46.1% among those egg negatives were antibody positive by the kits. Furthermore, 5.5%-18.8% of the egg positives were antibody negative by the kits.   Conclusion   The five sero-diagnostic kits can be used for epidemiological survey in schistosomiasis endemic areas at community level. Among them, enzyme-linked immunosorbent assay shows higher sensitivity.

Collective Group Sparganum and Validity of Spirometra erinacei

WEN Ting-huan

2010, 28(6):  10-444-450. 

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【Abstract】  Parasites and their hosts are animals， their scientific names are subject to the International Code of Zoological Nomenclature. The core principles of the Code are the Principle of Name?鄄Bearing Types， the Principle of Priority and the Principle of Homonymy. The Code provides guidance for zoologists to use correctly the available names. An assemb?鄄lages of identifiable species of uncertain generic position are treated as a genus?鄄group level for taxonomic convenience known as “collective groups” which requires no types and does not compete in priority with other genus-group names. The vernacular of plerocercoid metacestode of pseudophyllid tapeworm is known as sparganum， and has been treated as collective group Sparganum. The author makes a rectification for the Chinese terms of Diphyllobothrium and Spirometra according to their etymology. The original designation of the type species of the genus Spirometra is Diphyllobothrium decipiens （Diesing， 1850） by Faust et al， in 1929， but not Spirometra erinacei （Rudolphi） by Mueller in 1937， and the authorship of the latter species is Faust et al and the valid name as Spirometra erinacei （Faust， Campbell & Kellogg， 1929） synonymic with S. erinaceieuropaei （Rudolphi，1819） and Diphyllobothrium mansoni （Joyeux et Houdemer， 1928）， not vise versa， which is verified by CAB Thesaurus in 2010.

Research Progress on Nutrient Transport and Metabolism of Cryptosporidium

WANG Ju-hua，XUE Xiu-heng，LI Pei-ying

2010, 28(6):  11-451-454. 

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【Abstract】  Cryptosporidiosis is a worldwide zoonotic disease, and Cryptosporidium is coccidia-like parasite that develops in epithelial cells in digestive and respiratory tracts of human and animals. This review summarizes the specific function structure of Cryptosporidium， nutrient uptake, transport, metabolism, and the impact of Cryptosporidium on host nutrient absorption.

Research Progress on Antimicrobial Peptides against Malaria Parasite

DAI Yu-hua，HUO Hong-jie，WANG Huai-wei，GONG Mao-qing

2010, 28(6):  12-455-459. 

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【Abstract】  Antimicrobial peptides have broad-spectrum antibacterial activity and high thermal stability. Researches prove that they can inhibit the development of Plasmodium or kill them. The paper focuses on research advances in their biological characteristics, natural or synthetic peptides as potential anti-Plasmodium agents in malaria research.

Research Progress on Human Sarcocystosis

HU Jun-jie，MENG Yu，CHEN Xin-wen，ZUO Yang-xian，WU Peng

2010, 28(6):  13-460-467. 

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【Abstract】  Human sarcocystosis （both the intestinal and muscular forms） may be emerging as a significant， food-borne zoonotic infection in southeast Asia and southwest of China. This review summarizes recent findings in classification of Sarcocystis spp.， epidemiologic features， clinical symptoms， diagnosis， treatment and prevention in human infections.

The Immunopathology Mechanisms of Matrix Metalloproteinases in Brain Infection Induced by Parasites

SUN Rui，WU Zhong-dao

2010, 28(6):  14-468-473. 

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【Abstract】  Matrix metalloproteinase（MMP）family is a class of endogenous peptidases involved in normal physiological processes of the body， such as embryonic development， wound healing， angiogenesis， cartilage absorption，etc. However， during infection， over-expression of activated matrix metalloproteinase can lead to immunopathological change， induce disease or death， also contribute to the spread or survival of pathogens. Some human parasites such as Plasmodium， Toxoplasma gondii， Cysticercus cellulosae， Angiostrongylus cantonensis larvae can invade the central nervous system， and cause inflammatory response dominated by eosinophil infiltration. This article reviews function and pathological mechanism of MMP during the infection of relevant parasites involving brain.

Echinococcosis Prevalence in Elderly Population from Haiyan County of Qinghai Province

QIAO Hai-sheng，GUO Zhi-hong，LI Wei，DUO Hong，LI Zhi-ning，Kozo MATSUBAYASHI，Kiyohito OKUMIYA，DAI Qing-xiang，XU Hui-ning

2010, 28(6):  15-458-459. 

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【Abstract】  Echinococcus infection in elderly population（60-87 years old） was investigated by serological assay, medical imaging and epidemiology history in August, 2008. A total of 234 subjects from different nationalities were examined. Seven cases were serologically positive for echinococcosis and confirmed by medical imaging. Out of 30 suspected sera-positive cases, 3 were positive by medical imaging. There were 10 confirmed echinococcosis cases with a positive rate of 4.3%. There was significant difference on the prevalence among different towns（P＜0.05）, but no signifi-cant statistical difference on the prevalence among nationalities, careers and sex. The prevalence in females （4.8%） was higher than that of males （3.6%）.

Acquisition and Analysis of Lipophosphoglycan 1 Gene and the Noncoding Region of Leishmania donovani Isolates from Hilly Foci of China

LU Xiao-jun，YAN Ke-ning，JIAN Jun，DU Xiao-qing，MA Ying

2010, 28(6):  16-465-467. 

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【Abstract】  A fragment about 2.2 kb located at upstream of lipophosphoglycan 1 （LPG1） gene of Leishmania don-ovani isolate from hilly foci of China was obtained by PCR. The nucleotide sequence of the fragment was determined by sequencing. The sequence of the LPG1 gene and its downstream fragment （about 2 kb） was determined by using genome walking method. The above two fragment splicing result showed that the nucleotide sequence of the LPG1 gene with the noncoding region was 4 121 bp （GenBank accession number： HMO27899）. The BLAST results showed that the LPG1 gene of L. donovani isolate from hilly foci of China had 72%-78% nucleotide identity compared to that of other Leishmania spp. in GenBank.

Bioinformatics Analysis of COⅠ Gene of Simulium quinquestriatum

LUO Hong-Bin，YANG Ming，CHEN Han-Bin

2010, 28(6):  17-473-475. 

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【Abstract】  The genomic DNA was extracted from Simulium quinquestriatum （Sq） and its COⅠ gene was amplified by PCR. The PCR product was purified and cloned into plasmid pMD18-T vector. The recombinant plasmid was transformed into Escherichia coli DH5α and then identified by digestion with restriction enzyme and PCR amplification. The amplified frac-tions （1 621 bp） included complete COⅠ gene （1 542 bp, GenBank accession number： DQ534949）， 5′ tRNA-Tyr and 3′ tRNA-Leu partial fraction. The COⅠ gene sequence had a high identity （99%） with that of S. quinquestriatum （GenBank accession number： AY251520）. Bioinformatics analysis showed that the Sq-COⅠopen reading frame encoded a 513-amino acid protein with Mr 5 565, pI 5.84. Structural prediction showed this protein possessed a conservative domain of COⅠ gene.

Imported Falciparum Malaria Situation in Henan Province During 2005-2009

ZHANG Hong-wei，SU Yun-pu，ZHAO Xu-dong，YAN Qiu-ye，LIU Ying，CHEN Jian-she

2010, 28(6):  18-476-477. 

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【Abstract】  Imported falciparum malaria data of Henan Province in 2005-2009 were collected and statistically analyzed by using Microsoft Office Excel 2003. A total of 84 imported falciparum malaria cases with one death were reported during 2005-2009. The ratio of males to females was 20 ∶ 1（80/4）. The average age was （34±11） years old. Seventy-five patients returned from Africa, occupying 89.3%. The number of annual cases reported in 2008 （24 cases） and 2009 （32 cases） was higher than that in 2005-2007（9-10 cases）. The top three high-risk populations were farmers （34 cases）, workers （17 cases） and the cadre （13 cases）, accounted for 76.2% of all cases. The cases were reported every month, of which 37 cases（44.0%） were reported in June and July. All cases had a history of prior residence in falciparum malaria endemic area, and the initial symptom was fever. The average interval from symptom appearing to diagnosis was （5.9 ± 8.6） d, only 21.4% （18/84） of the patients was diagnosed within 24 h. There were 72 laboratory-confirmed cases（85.7%）. Seventeen cases （22.1%） were reported by county level medical treatment units. A total of 83 cases recovered with the treatment of artemisinin-based combination drugs. One case died with serious cerebral malaria. Effective measures should be carried to control the increasing trend of imported falciparum malaria.