SEQUENCE ANALYSIS OF APICAL MEMBRANE ANTIGEN Ⅰ FROM A PLASMODIUM FALCIPARUM ISOLATE COLLECTED FROM MENGPENG TOWNSHIP, YUNNAN PROVINCE

Abstract

Abstract: Genomic DNA from a Plasmodium falciparum isolate (CMP-1) collected from a falciparum malaria patient at Mengpeng Township, Mengla County, Yunnan Province was used as the template for PCR amplification primered with oligonucleotides from the conserved regions of the apical membrane antigen I (AMA-1) as reported. PCR products recovered from the low melting-point agarose gel electrophoresis were digested with BamHI and EcoRI to generate fragments approximately 420, 445 and 900 bp which were used as the inserts to insert into the M13 mp8 and M13 mp9 vectors for sequencing using 373 sequencer. 1773 bases were read out, among which 17 point mutations were found to result in substitutions of 15 codens which were non-synonymous mutations except for one as compared with that of the consensus. In particular, a relatively hot region of variation was apparent between amino acids 160 and 210.

CLC Number:

[1]	LIN Weina, DENG Wangping, YANG Ying, LI Yinlong, QIN Zhiqiang, HONG Yang, FENG Ting, LV Chao, XU Jing. Establishment and preliminary evaluation of a rapid assay for detection of Schistosoma japonicum nucleic acid based on LAMP-CRISPR/Cas12a [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 167-174.
[2]	XU Fangfang, CHEN Quan, HU Yuan, CAO Jianping. Changes of Tsc22d3 expression in NK cell in liver of mice infected with Schistosoma japonicum and the effect on the cytotoxicity of NK cells [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 175-180.
[3]	YANG Wanxuan, SHEN Haimo, CHEN Shenbo, CHEN Junhu. Analysis of the key sites of Plasmodium vivax VIR14 protein interacting with ICAM-1 receptor [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 192-197.
[4]	WU Yinjuan, YI Xueqing, LI Meiyu, XU Anyuan, WU Aoxun, ZHONG Zifeng, LI Xuerong. Effect and mechanism of Csi-miR-125a induced liver fibrosis in the exosomes of Clonorchis sinensis [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 198-204.
[5]	JING Tianyu, MOU Rutao, ZHANG Fan, LIU Xianbing, HU Xuemei, ZHANG Haixia, LI Zhidan. Effect of Toxoplasma gondii infection during pregnancy on CD73 expression in trophoblasts and decidual immune cells and the association of CD73 expression with adverse pregnancy [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 210-216.
[6]	ZHANG Xiaocheng, HU Yuan, PENG Hui, SHEN Yujuan, LIU Hua, CAO Jianping. CRISPR/Cas9-based generation of the bfd2 deficient strain of Toxoplasma gondii and analysis its phenotype [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 217-222.
[7]	PU Na, BO Xinwen, ZHAO Wenqing, CHEN Xuke, ZHANG Yuxia, ZHANG Yanyan, SUN Yan, WANG Zhengrong. Prokaryotic expression and preliminary functional identification of Echinococcus granulosus calreticulin [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 223-230.
[8]	CAO Yu, LI Ye, GAO Zhongyan, JIANG Botao. Isolation and identification of Patagifer bilobus [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 236-240.
[9]	WU Aoxun, ZHONG Zifeng, XU Anyuan, PAN Xiaowen, YI Xueqing, WU Yinjuan, LI Xuerong. Research progress on the mechanism of Clonorchis sinensis miRNAs in the initiation and progression of cholangiocarcinoma [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 269-273.
[10]	LI Benfu, XIAO Dan, LU Chunhua, SHI Shuai, YAN Xinliu, ZI Jinrong, PENG Jia, LI Jianxiong, WANG Zhengqing, XU Qian, WU Fangwei, YANG Yaming. Analysis of the infection factors Echinococcus granulosus in dogs in Shangri-La City, Yunnan Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 281-285.
[11]	YIN He, MA Lei, DANG Tiantian, LI Jiaming, Zhao Zhijun. Effect and mechanism of Toxoplasma gondii ROP16 Ⅰ/Ⅲ regulating TAF15 on THP-1 cells [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 103-111.
[12]	XU Xinjie, LI Jing, WU Yike, YANG Feng, SHAN Wenxin, ZHANG Zhiding, YE Changjiang, SUN Entao. RNA sequencing analysis of gene expression in Dermatophagoides farinae post-exposure to horseradish and the underlying mechanisms [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 112-118.
[13]	CUI Lei, FANG Yuan, ZHOU Zhengbin, HE Yaqi, ZHANG Yi. Research progress on pathogens carried by sand flies and their gut microbiota [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 129-134.
[14]	LI Guang, JIANG Huijiao, DU Yunfeng, SHU Min, LUO Yumeng, ZHU Lingyi, CHEN Xueling, WU Xiangwei. Interaction between mouse macrophages and protoscolex of Echinococcus granulosus in vitro [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 52-60.
[15]	DU Jianbo, SU Yaxin, HUO Lele, WANG Ying, WANG Xu, JIANG Bin, CHEN Yuqing, SHEN Yujuan. Establishment and application of a dual-probe fluorescent recombinase polymerase amplification assay for detection of Echinococcus granulosus [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 61-68.

SEQUENCE ANALYSIS OF APICAL MEMBRANE ANTIGEN Ⅰ FROM A PLASMODIUM FALCIPARUM ISOLATE COLLECTED FROM MENGPENG TOWNSHIP, YUNNAN PROVINCE

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments