Observation of the Feline Intestinal Epithelial Cell Infected with Toxoplasma gondii

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Observation of the Feline Intestinal Epithelial Cell Infected with Toxoplasma gondii

ZENG Jun-ling1，NA Ren-hua2，CHEN Ai-yuan2，WEI Hai-xia2，YAO Yun-ying2，PENG Hong-juan2，YANG Pei-liang 1*，CHEN Xiao-guang2

1 Nanfang Hospital；2 Department of Parasitology，School of Public Health and Tropical Medicine，Southern Medical University，Guangzhou 510515，China

Online:2015-02-28 Published:2015-05-04
Supported by:
Supported by Guangdong Natural Science Fund（No. S2012010009053），and the Guangdong Provincial Scientific and Technological Project（No. 2013B060300031）

Abstract

Abstract:

Small intestine samples of neonatal cat were aseptically collected from the jejunum-ileum region and digested with collagenaseⅪ/dispaseⅠ. Immunohistochemistry results showed that feline intestinal epithelial cells were successfully isolated and could be cultured. Cytokeratin was positive in the cytoplasm of feline intestinal epithelial cells. The cells were infected with the bradyzoites of Toxoplasma gondii Prugniaud strain， and the rupture of the cells was observed on the 72nd day post-infection. The sexual stage of T. gondii did not occur， however.

Key words: Toxoplasma gondii, Feline, Intestinal epithelial cell, Infection

ZENG Jun-ling1，NA Ren-hua2，CHEN Ai-yuan2，WEI Hai-xia2，YAO Yun-ying2，PENG Hong-juan2，YANG Pei-liang 1*，CHEN Xiao-guang2. Observation of the Feline Intestinal Epithelial Cell Infected with Toxoplasma gondii[J]. .

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Observation of the Feline Intestinal Epithelial Cell Infected with Toxoplasma gondii

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