Abstract: Cultured T.vaginalis was used for the anti-trichomonas test at different times, concentrations of propolis and densities of the parasites. After being cultured for 0, 6, 12, 24 hrs, the survival rate of the parasites was （91.50±3.11）%， （43.00±6.83）%， （22.25±5.32）% and （11.50±5.74）% respectively with a significant difference between propolis group and the control. Under the concentrations of 0.24, 0.48, 0.96, 1.92, 3.84 and 7.68 （mg/ml） with 24 hrs culture, the survival rate was （88.00±5.29）%， （92.67±4.16）%， （90.0±6.00）%， （84.00±4.00）%， （2.67±1.15）%， and 0 respectively. The results showed that propolis possesses clear in vitro anti-trichomonas activity which is relevant to the duration of culture and the concentration of the agent.

Key words: Propolis, Trichomonas vaginalis, Anti-trichomonas effect