Genetic identification on Meotrchis orientalis infection in human populations of Pucheng County, Fujian Province

doi:10.12140/j.issn.1000-7423.2024.06.019

Abstract

Abstract:

To understand the infection status of Meotrchis orientalis among the population in Pucheng County, Fujian Province and supplement the biological information of M. orientalis. Fecal samples of individuals infected with M. orientalis reported from the national clonorchiasis surveillance site of Pucheng County from 2016 to 2020 were collected. Modified Kato-Katz thick smear method (two slides per sample) was used to examine the eggs of M. orientalis. The DNA was extracted from fecal samples. The internal transcribed spacer (ITS) sequence of M. orientalis ribosomal DNA was amplified by PCR and sequenced. The positive rates of 2 methods were calculated and compared by Chi-square test. A total of 13 fecal samples of individuals infected with M. orientalis were collected. Among them, 11 tested positive using Kato-Katz method; Target bands at 262 bp were amplified using PCR method in 10 fecal samples, which showed a 99.6% identity to the ITS sequence of M. orientalis (MK482055). The positive rates of Kato-Katz method and PCR method were 11/13 and 10/13, respectively, with no statistically significant difference (χ² = 0.25, P > 0.05). The results indicated that there was an infection of M. orientalis among the population in Pucheng County, which was easily confused with Clonorchis sinensis infection. The ITS sequence used in this study can distinguish between M. orientalis and C. sinensis, and can be used as a genetic marker for M. orientalis.

Key words: Meotrchis orientalis, Infection, Molecular identification, Pucheng County

CLC Number:

R383.29

CHEN Yunhong, XIE Xianliang, CHEN Baojian, CHEN Fangmei, YU Wenwu. Genetic identification on Meotrchis orientalis infection in human populations of Pucheng County, Fujian Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(6): 810-812.

Figures/Tables 2

References 18

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特征	感染者人数	占比
地区
水北	3	3/13
临江	1	1/13
富岭	6	6/13
仙阳	3	3/13
性别
男	7	7/13
女	6	6/13
年龄组/岁
< 30	2	2/13
30~60	6	6/13
> 60	5	5/13
文化程度
小学	10	10/13
初中	2	2/13
高中	1	1/13
职业
农民	13	13/13