Diagnosis and analysis of an imported case of cutaneous leishmaniasis in Henan Province

Abstract

Abstract:

The male patient who went to Uzbekistan in June 2017 and found two small red rashes on the left thoracic arm on July 2 returned to China in October. The rashes became larger with a small amount of pus. A rapid strip test for leishmaniasis showed that the patient serum was negative for anti-Leishmania antibody. Microscopic examination of the lesioned tissue revealed no Leishmania amastigotes. The lesioned tissue was cultured in NNN medium and a large number of promastigotes were found in culture on day 8. The promastigote DNA was extracted and underwent PCR amplification using Leishmania-specific primers K13A/K13B and L5.8S/LITSR, producing fragments of 120 and 350 bp. The two products were 90% and 98% homologous to the corresponding sequences of L. major (GenBank accession number: EU370906.1 and FN677342.1). The patient was diagnosed as imported cutaneous leishmaniasis and the pathogen was identified to be L. major. The disease was controlled after 2 treatment courses with sodium stibogluconate at a total dosage of 7.2 g (6 days for each course, with a 1-week interval between them). The patient had recovered by the end of the study.

Key words: Cutaneous leishmaniasis, Promastigote, Leishmania major, Diagnosis

CLC Number:

R531.62

Su-hua LI, Li-jun GAO, Ya-lan ZHANG, Rui-min ZHOU, Cheng-yun YANG, Dan QIAN, Ying LIU, Yu-ling ZHAO, De-ling LU, Hong-wei ZHANG, Bian-li XU. Diagnosis and analysis of an imported case of cutaneous leishmaniasis in Henan Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(4): 339-342.

Figures/Tables 4

References 18

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