Investigation of Anisakis spp. larva infection in marine fish for sale in Yantai City

Abstract

Abstract:

Objective To investigate the infection status of marine fish sold in Yantai City by Anisakis spp. Methods Marine fish of different species were randomly purchased from seafood markets in Yantai City from January to December 2016. The fish were dissected to find and collect Anisakis larvae from the viscera and muscle. The larvae species were identified under a light microscope. The Anisakis larvae infection status in fish was analyzed in different aspects of fish species, visceral organs and fish body weight. Results A total of 256 marine fishes of 14 species were dissected. The overall infection rate was 66.4% (170/256) and the average infection intensity was 14.8 larvae/fish. Those fishes with top infection rate included Pneumatophorus japonicas (100%, 26/26), Trichiurus lepturus (92.3%, 24/26), Platycephalus indicus (92.0%, 23/25) and Sebastodes fuscescens (85.7%, 18/21). The average infection intensity of them was 25.1, 21.1, 12.2, and 24.9 larvae/fish, respectively. Other species with comparatively lower infection rate were Monopterus albus (80.0%, 6.3 larvae/fish), Prognichthys agooz (70.0%, 3.3 larvae/fish), Larimichthys polyactis (66.0%, 11.7 larvae/fish), Scomberomorus niphonius (52.0%, 9.1 larvae/fish), Cleisthenes herzensteini (50.0%, 1.8 larvae/fish), Clupea pallasi (44.4%, 9.5 larvae/fish) and Tylosurus melanotus (38.1%, 2.0 larvae/fish). No infection was found in Pampus argenteus, Thamnaconus modestus and Perca fluviatilis. The Anisakis spp. larvae were mainly located at mesentery and intestinal walls as well as gastric wall and gonad. There were also varied relationships between infection intensity and weight among different fish species. The infection intensity of 0.25-0.35 kg P. japonicus, 0.16-0.20 kg T. lepturus, 0.16-0.25 kg P. indicus, 0.05-0.10 kg L. polyactis, 0.17-0.26 kg S. fuscescens and 0.81-1.00 kg S. niphonius was 46.5, 27.2, 18.0, 12.0, 42.7 and 68.0 larvae/fish, respectively. Among those species, the infection intensity decreased with the increase of body weight in S. fuscescens (r = 0.484, P < 0.05) and L. polyactis (r = -0.633, P < 0.01), but increased with body weight in S. niphonius (r = 0.740, P < 0.01). However, for P. japonicas, T. lepturus and P. indicus, neither relationship was found (r = -0.237, -0.080, -0.196, P < 0.05). Conclusion There is a high infection rate (above 85%) of Anisakis larvae in P. japonicas, T. lepturus, P. indicus and S. fuscescens from the seafood markets in Yantai City.

Key words: Marine fish, Anisakis spp., Infection, Yantai City

CLC Number:

R383.1

Wen-qian ZHANG, Xiao-tong REN, Yu-qi ZHAO, Xin-xin GAI, Yu-mei ZHANG, Xue-lian BAI. Investigation of Anisakis spp. larva infection in marine fish for sale in Yantai City[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(5): 472-477.

Figures/Tables 5

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References 22

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鱼种Fish species	平均体长/cm Average length/cm	平均体质量/g Average weight/g	检查数 No. examined	感染数 No. Infected	检获虫数 No. Anisakis	感染率/% Infection rate/%	感染度/条·鱼^-1 Infection intensity/No. Anisakis per fish
鲐鱼P. japonicus	34.9	476.2	26	26	653	100	25.1
带鱼T. lepturus	66.8	157.9	26	24	506	92.3	21.1
辫子鱼P. indicus	29.9	201.1	25	23	281	92.0	12.2
黑鲪S. fuscescens	19.4	179.5	21	18	449	85.7	24.9
海鳗M. albus	37.5	83.0	5	4	25	80.0	6.3
真燕鳐P. agooz	23.8	133.5	10	7	23	70.0	3.3
小黄鱼L. polyactis	19.7	93.8	50	33	386	66.0	11.7
蓝点马鲛S. niphonius	39.1	401.2	25	13	118	52.0	9.1
高眼鲽C. herzensteini	22.7	103.3	20	10	18	50.0	1.8
鲱鱼C. pallasi	29.5	262.6	9	4	38	44.4	9.5
黑背圆颌针鱼T. melanotus	42.0	121.6	21	8	16	38.1	2.0
银鲳P. argenteus	20.1	225.8	9	0	0	0	0
绿鳍马面鲀T. modestus	28.9	276.8	6	0	0	0	0
海鲈鱼P. fluviatilis	42.0	941.1	3	0	0	0	0
合计Total	-	-	256	170	2 513	66.4	14.8

鱼种 Fish species	感染率/% Infection rate%
鱼种 Fish species	体腔 Body cavity	胃壁 Gastric wall	肠系膜及肠壁 Mesentery and intestinal wall	肝、胰 Liver and pancreas	生殖器 Gonad	肌肉 Muscle	心脏 Cardiac	鳃 Branchia
鲐鱼P. japonicus	51.8	12.7	27.6	2.5	2.1	2.1	0.5	0.8
带鱼T. lepturus	36. 6	3.2	29.1	1.2	29.8	0.2	0	0
辫子鱼P. indicus	1.1	37.0	55.9	3.9	0.4	1.1	0	0.7
小黄鱼L. polyactis	33.0	17.9	25.7	12.4	6.0	2.9	1.0	1.3
黑鲪S. fuscescens	3.6	23.8	69.3	3.3	0	0	0	0
海鳗M. albus	16.0	12.0	60.0	12.0	0	0	0	0
真燕鳐P. agooz	30.4	26.1	17.4	26.1	0	0	0	0
蓝点马鲛S. niphonius	55.1	8.5	26.3	1.7	3.4	5.1	0	0
高眼鲽C. herzensteini	16.7	0	66.7	16.7	0	0	0	0
鲱鱼C. pallasi	10.5	52.6	26.3	2.6	7.9	0	0	0
黑背圆颌针鱼T. melanotus	31.3	6.3	25.0	31.3	0	6.1	0	0

鱼种 Fish species	鱼体质量/kg Fish mass/kg	检查数 No. examined	感染度/条·鱼^-1 Infection intensity/No. Anisakis per fish
鲐鱼 P. japonicus	0.25~0.35	2	46.5
鲐鱼 P. japonicus	0.36~0.45	5	29.6
	0.46~0.55	13	14.4
	0.56~0.65	6	37.5
带鱼 T. lepturus	0.10~0.15	14	19.7
带鱼 T. lepturus	0.16~0.20	9	27.2
	0.21~0.25	1	7.0
	0.26~0.30	2	9.0
辫子鱼 P. indicus	0.05~0.15	9	9.5
辫子鱼 P. indicus	0.16~0.25	11	18.0
	0.26~0.35	1	2.0
	0.36~0.45	4	1.7
黑鲪 S. fuscescens	0.06~0.16	12	25.7
黑鲪 S. fuscescens	0.17~0.26	3	42.7
	0.27~0.36	1	12.0
	0.37~0.46	5	8.7
小黄鱼 L. polyactis	0.05~0.10	33	12.0
小黄鱼 L. polyactis	0.11~0.15	6	0
	0.16~0.20	9	1.0
	0.21~0.25	2	0
蓝点马鲛 S. niphonius	0.20~0.40	19	2.3
蓝点马鲛 S. niphonius	0.41~0.60	3	4.5
	0.61~0.80	2	11.5
	0.81~1.00	1	68.0