Discovery of Culex inatomii（Diptera ∶ Culicidae）in Chongming, Shanghai

Abstract

Abstract:

Objective To record the discovery of Culex inatomii in Chongming, Shanghai. Methods Larvae and adult mosquitos of Cx. inatomii were collected in Dongtan of Chongming Island from May to November in 2015 and 2016, and their morphological characteristics were observed. The genomic DNA was extracted from adult mosquitos, PCR was performed to amplify the cytochrome c oxidaseⅠ(COⅠ) gene. Multiple alignment of COⅠ sequence was conducted with ClustalW2. Pairwise distances within and between species were calculated using MEGA v5.10 based on COⅠ sequences of Cx. inatomii, Cx. modestus, Cx. pipiens pallens, and Cx. tritaeniorhynchus. The phylogenetic tree of the above four species was constructed using neighbor joining, maximum likelihood, and Bayesian methods. Results One hundred and fifty-six adult female mosquitos and 36 larvae of Cx. inatomii were collected. Larvae were reared to adult stage in the laboratory (17 female, 19 male). Morphologically, the subapical lobe of the sidepiece in male genitalia was divided into two parts, the anterior part having 2 bladed setae, and the posterior part having 1 bladed setae and 1 lanceolar strong setae. This strucutre can be used to distinguish Cx. inatomii from Cx. modestus. PCR of COⅠ resulted in products of approximately 650 bp. They were sequenced and the sequencing result was submitted to GenBank (accession number, KX555565-KX555570). Multiple sequence alignment revealed a 96% sequence similarity of COⅠ between Cx. inatomii and Cx. modestus. The genetic distance between Cx. inatomii and Cx. modestus was 0.047, and that within them each was 0.003 and 0.011, respectively. The phylogenetic tree showed that the four species clustered as a monophyletic clade, and each formed an individual lineage. Cx. inatomii had a closer relationship with Cx. modestus, while distant from the other two species. Conclusion We recorded the discovery of Cx. inatomii in Chongming, Shanghai.

Key words: Culex inatomii, Culex modestus, Shanghai, Discovery

CLC Number:

R384.112

Yuan FANG, Wen-qi SHI, Yi ZHANG, Qiu-an HU, Zheng-bin ZHOU, Jia-tong WU, Liang-liang ZHANG. Discovery of Culex inatomii（Diptera ∶ Culicidae）in Chongming, Shanghai[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(1): 31-35.

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蚊种 Species	稻富库蚊 Cx. inatomii	三带喙库蚊 Cx. tritaeniorhynchus	淡色库蚊 Cx. pipiens pallens	凶小库蚊 Cx. modestus
稻富库蚊 Cx. inatomii	0.003
三带喙库蚊 Cx. tritaeniorhynchus	0.107	0.008
淡色库蚊 Cx. pipiens pallens	0.076	0.098	0.001
凶小库蚊 Cx. modestus	0.047	0.116	0.069	0.011