Molecular Detection of Giardia lamblia and Cryptosporidium Species in Pet Dogs

Abstract

Abstract:

Objective To determine the prevalence of Giardia lamblia and Cryptosporidium species infection in pet dogs, and identify the G. lamblia assemblages and Cryptosporidium species. Methods A total of 315 fresh fecal samples were collected from pet clinics in five counties of Anhui Province and in Hangzhou of Zhejiang Province. Hemi-nested-PCR targeting the GDH gene of G. lamblia and nested-PCR targeting the SSU rRNA gene of Cryptosporidium were performed in all the fecal samples. The PCR products were sequenced and analyzed using bioinformatics methods to identify the G. lamblia assemblages and Cryptosporidium species. Results The positive rates of G. lamblia and Cryptosporidium spp. infections in the 315 fecal samples were 3.2% （10/315） and 1.6% （5/315）, respectively. Specifically, the two indicators were both significantly higher in dogs ≤12 months （17.8% and 11.1%, respectively） than in adult dogs （0.7% and 0.0%）（P<0.05）. However, there was no significant difference in the two indicators between male and female dogs. In addition, two G. lamblia assemblages were identified, assemblages B（n=6） and D（n=4）. Sequence analysis of PCR products of the SSU rRNA gene showed that the five Cryptosporidium isolates were C． canis（n=5）. Conclusion The prevalences of G. lamblia and Cryptosporidium infection in pet dogs in Anhui and Zhejiang Provinces were 3.2 % and 1.6 %, respectively. The assemblages of G. lamblia in this study are of types B and D.

Key words: Giardia lamblia, Cryptosporidium, Pet dog, Anhui, Zhejiang

GU You-fang, WANG Kai, LIU De-yi, MEI Nan, CHEN Cheng, CHEN Tao, HAN Min-min, ZHOU Li, CAO Jia-tong, ZHANG Heng, ZHANG Xue-liang, FAN Zi-lai, LI Wen-chao*. Molecular Detection of Giardia lamblia and Cryptosporidium Species in Pet Dogs[J]. .

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[2]	TENG Xuejiao, HUANG Hao, ZHU Jianhui, ZHONG Chen, CHEN Chen, WU Jiabing, DENG Shu. Analysis of notifiable parasitic diseases cases reported to the National Notifiable Infectious Disease Reporting System in Anhui Province from 2013 to 2023 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(1): 91-96.
[3]	WANG Jipu, ZHANG Yujin, WU Xinghua, WANG Mingxing, ZHU Yuqiao, LUO Hui, GAO Sichao, LIU Xinchao, LI Wenchao. Molecular detection and genetic characteristics of Cryptosporidium spp. in Equus animals in some areas of Anhui Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(6): 796-801.
[4]	ZHOU Shanshan, JIANG Yanyan, CAO Jianping. Cryptosporidium infection in rodents in China [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(4): 512-520.
[5]	MIN Xiangyang, FENG Meng, ZHAO Xuhong, WENG Wenhao, LI Hanhua. Diagnosis of a case of Giardia lamblia infection [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 272-274.
[6]	YANG Ling, WANG Long, WANG Jiayang, ZHOU Kunzheng, YAN Baolong, ZHAO Wei, HUANG Huicong. Expression of Cryptosporidium parvum GP900^829-1099 protein and its immunomodulatory effects on mouse macrophages cells [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(1): 55-62.
[7]	WANG Dan, HE Zhiquan, LIU Ying, LIU Lingzhi, CHEN Huihui, JIANG Tiantian, JI Penghui, QIAN Dan, YANG Chengyun, ZHANG Hongwei. Molecular identification and genetic tracing of Giardia lamblia isolated from an infected case [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 380-383.
[8]	QIN Yuan, LIU Hua, JIANG Yanyan, CAO Jianping, SHEN Yujuan. Molecular identification and genetic characterization of Cryptosporidium spp. in patients with diarrhea in Ningbo City [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 202-208.
[9]	CAO Zhiguo, WANG Tianping, JIN Wei, GUO Jianduo, ZHU Lei, LIU Daohua, WANG Min, LI Qiyang, HU Mingchuang. Surveillance of human soil-transmitted nematode infection in Anhui Province during 2016—2020 [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(1): 44-51.
[10]	LI Teng, SHEN Yu-juan, CUI Li-jun, LIU Hua, HU Yuan, JIANG Yan-yan, CAO Jian-ping. Long non-coding RNA NEAT1 involves in intestinal epithelial cell response against Cryptosporidium parvum infection via regulating IL-8 expression [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(4): 487-492.
[11]	CHEN Yuan-cai, HUANG Jian-ying, LI Jun-qiang, ZHANG Long-xian. Molecular epidemiology and subtype distribution of Cryptosporidium parvum from dairy cattle in China [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(3): 278-284.
[12]	ZHU Ming-chao, ZHU Ya. HIV co-infection with Blastocystis hominis and Giardia lamblia in a patient [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(3): 315-318.
[13]	LI Shu-ning, LI Wen-lin, SHEN Hai-e, WANG Yang, TIAN Xi-feng. Giardia lamblia trophozoites induce the formation of neutrophil extracellular traps in vitro [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(4): 455-460.
[14]	YU Ming-chuan, YANG Zhong-wei, WANG Hua-ran, SHI Dan-yang, ZHOU Shu-qing, YIN Jing, SUN Shu-min. Establishment of a Giardia lamblia detection method based on the LAMP microfluidic chip [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(3): 402-405.
[15]	JIANG Yan-yan, YUAN Zhong-ying, SHEN Yu-juan, XU Ning, ZHANG Ren-li, HUANG Da-na, CAO Jian-ping. Molecular characteristics of Cryptosporidium sp. isolated from imported breeding dairy cows in a dairy farm in Shenzhen, China [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(6): 718-722.

Molecular Detection of Giardia lamblia and Cryptosporidium Species in Pet Dogs

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