Abstract:

【Abstract】 Objective  To observe the microstructure and ultrastructure of Multiceps multiceps from the artificially infected dogs.  Metheds  Two male dogs were infected with the coenurus of M. multiceps from naturally-infected sheep（about 80-100 per dog）. The adult worms of M. multiceps were recovered from the intestine, and fixed by the conventional method. The scolex, neck,immature proglottid, mature proglottid, and gravid proglottid were prepared for paraffin section and ultrathin sections with HE staining and uranyl acetate staining, and observed under light microscope and electron transmission microscope, respectively.  Results  Under light microscope, each proglottid consisted of cortical layer and parenchymal layer. The cortical layer was composed of microvilli, syncytium, and substrate layer. The parenchymal layer mainly consisted of muscle tissue, excretory system, and reproductive system. The microvilli layer of scolex was thinner than that of neck and mature proglottid, and the longest microvilli were mainly distributed in the binding site between the proglottids. The scolex was extremely muscular. The nervous system and excretory system were repeated in each proglottid. Mature proglottid had both male and female reproductive systems. Gravid proglottid had uterus and egg, and atrophic male reproductive organs.  Conclusion  The special microstructure of Multiceps multiceps are that most microvilli in the cortex is cylindrical; the microvilli length in the binding sites between mature proglottids is longer than that of other parts.

Key words:  Dog, Multiceps multiceps, Histologic structure, HE stain, Ultrastructure