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mRNA Expression Analysis of 10 Reproduction-related Genes in Different Developmental Proglottids of Moniezia expansa

WANG Zheng-rong1，BO Xin-wen1 *，ZHANG Yan-yan1，MA Xun2   

  1. 1 Xinjiang Academy of Agricultural and Reclamation Science；Key Labaratory of Sheep Breed and Biotechnology of Xinjiang Production and Construction Corps，Shihezi 832000，China；2 Shihezi University，Shihezi 832000，China
  • Online:2015-02-28 Published:2015-05-04
  • Supported by:

    Supported by the Science and Technique Fund of Xinjiang Production and Construction Corps（No. 2014BA002），the National Natural Science Foundation of China（No. 31360608），the National Basic Research Program of China（No. 2015CB150300），and the Open Project of Key Labaratory of Sheep Breed and Biotechnology of Xinjiang Production and Construction Corps（No. 2013KLS06）

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Abstract:

According to the results of transcriptome sequencing in Moniezia expansa， 10 functional genes which represent different expression patterns in different developmental proglottids were selected， including KIFC1， Kif17， tgf-beta， SmadD， tgf-beta receptor， HSD5， aminopeptidase puromycin， Methionine aminopeptidase 2， transcription factor fork head and Sox transcription factor. A real-time fluorescent quantitative PCR assay was developed for detection of mRNA expression level of these taget genes with its beta-tubulin as an internal control. The results showed that compared to scolex-neck proglottids， the mRNA levels of 2 genes（KIFC1， Kif17） in immature proglottides， 4 genes（KIFC1， Kif17， tgf-beta receptor， and aminopeptidase puromycin） in mature proglottides， and 3 genes（HSD5， tgf-beta receptor， and Methionine aminopeptidase 2） in gravid proglottides were up-regulated （P<0.01）.

Key words: Moniezia expansa, Proglottid, Real-time PCR