›› 2014, Vol. 32 ›› Issue (3): 15-185-187.

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Identification of Three Common Sandfies in Southern Xinjiang with Multiplex PCR

ZHOU Zheng-bin1，ZHANG Yi1 *，ZHU Huai-min2，SHI Wen-Qi1，JIN Chang-Fa1   

  1. 1 National Institute of Parasitic Diseases，Chinese Center for Disease Control and Prevention；Key Laboratory of Parasite and Vector Biology，MOH；WHO Collaborating Center of Malaria，Schistosomiasis and Filariasis，Shanghai 200025，China；2 Department of Pathogenic Biology，The Second Military Medical University，Shanghai 200433，China
  • Online:2014-06-30 Published:2014-09-17

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Abstract: Based on the variable part of mtDNA COⅠ gene sequence， a multiplex PCR method was developed for the identification of the three common sandflies（Phlebotomus longiductus， Ph. wui， and Ph. alexandri） in southern Xinjiang. The results demonstrated that this multiplex PCR method was reliable， and could be used to identify the three Phlebotomus species. The PCR product of COⅠ gene from Ph. longiductus， Ph. wui and Ph. alexandri was 248, 632， and 395 bp， respectively.

Key words: Multiplex PCR, Phlebotomus longiductus, Phlebotomus wui, Phlebotomus alexandri, China