Abstract: Different concentrations of dimethylsulfoxide （DMSO） and glycerol， various densities of Trichomonas vaginalis trophozoites and duration of storage were used to study the effects on the cryopreservation of T. vaginalis at -78 ℃. The optimal densities of trichomonads for cryopreservation were （1-2） ×10⁶/ml. Glycerol and DMSO showed a high protective effect， with a maximum effect at a concentration of 10%， and the survival rates were 38.0% and 31.7%， respectively. There was no significant difference between them（P＞0.05）. The results showed that the above optimized factors can provide a good protection in short-term （1-16 weeks） cryopreservation， and the trichomonads can be incubated to a logarithmic growth phase at 37 ℃.

Key words: Trichomonas vaginalis, Cryopreservation, Dimethylsulfoxide, Glycerol