›› 2010, Vol. 28 ›› Issue (5): 18-394.

• 研究简报 • Previous Articles     Next Articles

Construction and identification of a full-length cDNA library from Spirometra erinaceieuropaei

LV Gang*，LU Ya-jun，FAN Zhi-gang，SHI Da-zhong，GAN Xiu-feng，ZHONG Sai-feng   

  1. Department of Parasitology，Hainan Medical College，Haikou 571101，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-30 Published:2010-11-09
  • Contact: LV Gang

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Abstract: The full-length pBluescript Ⅱ SK cDNA library of adult Spirometra erinaceieuropaei was constructed by using the SMART method. Data showed that 95.5% of the library was recombinant and the titer of the library was 1.06×106. The average insert size of the library was about 1.4 kb. Forty-eight randomly selected clones were sequenced. A set of 36 effective expressed sequence tags （ESTs） with the average size of 674 bp was obtained after excluding clones shorter than 450 bp. The unigenes occupied 58.3% of the 36 ESTs. The rate of full-length cDNAs were 57.7% （15/26）. The high-quality of full-length cDNA library could be used for large scale EST sequencing.

Key words: Spirometra erinaceieuropaei, Adult, Full-length cDNA Library