Abstract: A number of flies around the eyes of a person or around a fruit bait were collected from Huangshan Mountain, and experimentally infected by newborn larvae of Thelazia callipaeda. After 20 days, the flies were examined for T. callipaeda. Following dissection, 3 （30%, 3/10） of Amiota magna, and 55 （21.6%, 55/255） of A. okadai were found infected by T. callipaeda. The susceptibility of T. callipaeda is similar in the two species fruit flies （χ²=0.058 4， P>0.05）. The rabbits were infected by infective larvae of T. callipaeda from A. magna. At the 35th day after infection, the newborn larvae and worms of T. callipaeda were found in the conjunctival sac of rabbits. This study suggested that A. magna acts as intermediate host of T. callipaeda under laboratory conditions.

Key words: Amiota magna, Thelazia callipaeda, Susceptibility, Intermediate host