›› 2008, Vol. 26 ›› Issue (4): 11-289.

• 实验研究 • Previous Articles     Next Articles

Species Identification of Anopheles maculatus Complex in Malaria Endemic Area Motuo County, Tibet

WU Song1,3,PAN Jia-yun1,WANG Xue-zhong2,ZHOU Shui-sen1,
ZHANG Guo-qing,TANG Lin-hua1 *
  

  1. 1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Coll-aborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China;2 Yunnan Institute of Parasitic Diseases, Puer 665000, China;3 School of Integrated Traditional and Western Medicine, Anhui College of Chinese Traditional Medicine, Hefei 230038, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-08-30 Published:2008-08-30

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Abstract: Objective To identify the species of Anopheles maculatus complex in malaria endemic area Motuo County， Linzhi Prefecture， Tibet Autonomous Region. Methods 5 190 adult mosquitoes were morphologically identified as An. maculatus complex， and 575 were randomly selected to extract DNA by phenol-chloroform method. According to the rDNA ITS2 variations of An. maculatus s. s.， An. willmori， An. pseudowillmori， An. sawadwongporni and An. dravidicus， 5 pairs of specific primers were designed for PCR identification on the species of An. maculatus complex. The PCR products were sequenced in double directions， and homology searches were done over the Web using the program Blast. 22 ITS2 sequence of An. maculatus complex from GenBank was adopted to construct phylogenetic tree with UPGMA method by MEGA（3.1） software. Results 575 DNA samples were extracted. Among which， 11 were amplified 231 bp An. willlmori fragment （1.9%） and 564 were amplified 119 bp An . pseudowillmori fragment （98.1%）. PCR identification， Web homology blast and phylogenetic tree showed same results. Conclusion The predominant anopheline mosquitoes in Motuo County is An. pseudowillmori.

Key words: Tibet, Motuo, Anopheles maculatus complex, Species identification