Abstract: Objective To establish a standard method on collection and identification of Piroplasma infected to cattle and sheep. Methods The Piroplasma were isolated by inoculating the blood or tick collected from the field, the tick vectors were determined by transmission experiment; The phylogenetic relationship was studied by sequencing 18S rRNA gene. Results Thirty four isolates of Piroplasma were obtained from the distinct areas of China, and some studies on their taxonomy were carried out. Conclusion Piroplasma could be effectively isolated by the blood inoculation or by the transmission with ticks, more accurate taxonomic studies were obtained by the classical method in combination with the molecular method.

Key words: Piroplasma, Tick, Infection, Sequencing of 18S rRNA gene, Collection, Identification