›› 2006, Vol. 24 ›› Issue (3): 21-VI.

• 研究简报 • Previous Articles     Next Articles

Construction of Prokaryotic Expression Vector for Trichomonas vaginalis Silent Information Regulator 2 and Its Expression

ZHANG Ke-hao1;HUANG Li-xia1;FU Yu-cai2;ZHANG Jia-xin3   

  1. 1 Taizhou Hospital of Zhejiang Province，Taizhou 317000，China; 2 Shantou University Medical College，Shantou 515041，China; 3 Xianyue Hospital，Xiamen 361012，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-06-30 Published:2006-06-30
  • Contact: FU Yu-cai

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Abstract: Total RNA was isolated from Trichomonas vaginalis and Tv-Sir2-like cDNA was amplified by RT-PCR and cloned into pGEM-T Easy plasmid. A fragment of Tv-Sir2-like cDNA was subcloned into the expression vector pET-41b and expressed in E.coli BL21 with induction of IPTG. The full-length of Tv-Sir2-like cDNA was cloned and sequenced. The prokaryotic expression system of pET-41b/Tv-Sir2-like was constructed. The fusion protein of Tv-Sir2-like was expressed in E.coli BL21，occupying 30% of the total bacterial protein after being induced by IPTG for 5 h. SDS-PAGE analysis showed that the fusion protein was about Mr 59 000. The recombinant protein of Tv-Sir2-like is efficiently expressed in E.coli BL21.

Key words: Trichomonas vaginalis, Sir2, homolog, Gene cloning, Prokaryotic expression