Sequence Analysis of ITS2 and CO1 Genes of Paragonimus harinasutai

›› 2006, Vol. 24 ›› Issue (2): 9-121.

• 实验报道 • Previous Articles Next Articles

Sequence Analysis of ITS2 and CO1 Genes of Paragonimus harinasutai

QIAN Bao-zhen1;H.Sugiyama2;J.Waikagul3;ZHU Zhi-hang4

1 Institute of Bio-engineering，Zhejiang Academy of Medical Sciences，Hangzhou 310013，China；2 Department of Parasitology，National Institute of Infectious Diseases，Japan；3 Department of Tropical Medicine，Mahidol University，Thailand；4 Ninghai CDC，Ninghai 315600，China

Received:1900-01-01 Revised:1900-01-01 Online:2006-04-30 Published:2006-04-30

Abstract

Abstract: Objective To identify Paragonimus harinasutai from Ninghai， Zhejiang Province， China. Methods Meta-cercariae were collected from the crabs Sinopotamon chekiangenes in Xixi village of Ninghai County for ITS2 sequence analysis， CO1 sequence analysis and endonuclease BsaHI and StuI analysis by PCR-RFLP. Results The fingerprintings of PCR-RFLP were virtually same to the isolate from Thailand （Nakorn-nayok）. The ITS2 sequence with 366 bp and CO1 sequence with 390 bp of the metacercariae collected from Ninghai revealed a nucleotide identity 95.6% and 89.5% respectively to the Thai isolate. Conclusion The study confirmed that Paragonimus harinasutai is present in Ninghai， China， with certain variation on molecular biology in comparison to the Thai isolate.

Key words: Paragonimus harinasutai, Classification, Sequence analysis

QIANBao-zhen;H.Sugiyama;J.Waikagul;ZHUZhi-hang. Sequence Analysis of ITS2 and CO1 Genes of Paragonimus harinasutai[J]. , 2006, 24(2): 9-121.

[1]	LI Benfu, WANG Zhengqing, XU Qian, ZI Jinrong, YAN Xinliu, PENG Jia, LI Jianxiong, CAI Xuan, WU Fangwei, YANG Yaming. Sequence analysis of mitochondrial co1 and nd1 genes in Echinococcus granulosus in Yunnan Province [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(3): 306-311.
[2]	LIU Ya-fang, CHEN Bin, LU Xin-yan, LI Guang-hua, DU Chun-hong, JIANG Dan-dan, YANG Xing. Complete mitochondrial genome sequence of Rhipicephalus microplus [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(5): 677-681.
[3]	LI Hai-yun. Classification system adjustment of Pseudophyllidea and the identification characteristics of the main taxa [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(3): 411-419.
[4]	LIU Jian-xiu, WANG Jun-yun. Applications of molecular techniques in classification and identification of Leishmania [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(4): 508-513.
[5]	Cheng-yan ZHOU, Jun MA, Wei LIU, Qian-ming XU, Xing-quan ZHU. Sequence polymorphisms within Kalicephalus indicus ribosomal and mitochondrial genes [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(1): 81-86.
[6]	CHEN Mu-xin1,2, CHEN Jia-xu2, LIU Wei2, FENG Xin-yu2, CHEN Shen-bo1,2,CHEN Shao-hong2, CAI Yu-chun1,2, XU Bin2, HU Wei1,2*. Sequencing and Phylogenetic Tree Construction of 18S Ribosomal DNA from Five Species of Plasmodium from Yunnan Border between China and Myanmar and Other Areas [J]. , 2016, 34(6): 4-493-499.
[7]	FANG Yuan, SHI Wen-qi, ZHANG Yi. Research Progress in Classification of Anopheles hyrcanus* Group （Diptera ∶ Culicidae） [J]. , 2016, 34(6): 16-565-570.
[8]	SHEN Hai-mo, AI Lin, CAI Yu-chun, LU Yan, CHEN Shao-hong*. Digital Description and Identification of 11 Kinds of Principal Parasite Eggs [J]. , 2016, 34(5): 8-424-429.
[9]	ZHANG Shao-hua, LUO Xue-nong, LI Xue-qiang, CAI Xue-peng*. Cloning of Taenia pisiformis Actin Gene and Assessment of Its Use as An Internal Control [J]. , 2016, 34(1): 6-32-39.
[10]	ZHENG Sheng-bang，ZHOU Yi-biao*，LI Lin-han，WU Jin-yi，SONG Xiu-xia，JIANG QING-wu. Hemocyte Morphology and Classification in Oncomelania hupensis [J]. , 2014, 32(2): 16-91-94.
[11]	GENG Ying-zhi1, MAO Ling-ling1, TENG Cong1, AN Chun-li2, WANG Bo1, CHEN Jing-yi1, YAO Wen-qing1 . Allele Genetype Analysis of Plasmodium vivax* Merozoite Surface Protein1 (PvMSP-1) from Dangdong，Liaoning Province [J]. , 2012, 30(3): 18-242-244.
[12]	XU Xue-nian，ZHOU Yan，DONG Yu-ting，TAN Yu-guang，BAO Yi-fang，XU Bin1，CHENG Na，XU Hong-bo，LI Xue-ming，FENG Zheng. Cloning, Expression of PPMP Antigen Genes of Clonorchis sinensis and Immunogenic Identification of the Recombinant Proteins [J]. , 2010, 28(6): 1-401-405.
[13]	DANGZhi-sheng;LUOJian-xun;LIUAi-hong;BAIQi;GUANGui-uan;LIUZhi-jie;MAMi-ling;LIYou-quan;GAOJin-liang;SUGIMOTOChihiro;YINHong*. Sequence Analysis of Theileria annulata Surface Protein in Chinese Isolates [J]. , 2010, 28(3): 10-209.
[14]	LVGang;LIJing-Jing;ZHAOShi-Yong;LUYa-Jun;FANZhi-Gang. Molecular Cloning and Sequence Analysis of Lactate DehydrogenaseGene from Plasmodium vivax* Hainan Isolate [J]. , 2010, 28(1): 17-74.
[15]	QUFeng-yi;ZHUHuai-min. On Revision of the Tribe Aedini Mosquitoes Taxa Record in China, with a Proposed New Classification System （Diptera∶ Culicidae） [J]. , 2009, 27(5): 11-447.

Sequence Analysis of ITS2 and CO1 Genes of Paragonimus harinasutai

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments