Abstract: Objective To construct the recombinant plasmid P^PICZαA-Ts87 and obtain secretive protein in Pichia pastoris. Methods Ts87 was amplified by PCR and cloned into the expression vector P^PICZαA of P. pastoris to form P~PICZαA-Ts87. After the recombinant was isolated and linearized, it was transformed into P. pastoris GS115 by EasyComp^TM kit and screened for zeocin resistant with different conditions and Mut phenotype. The high resistant Mut~+ clones were cloned and the product induced by methanol was tested by dot-hybridization. The expression product was further identified by SDS-PAGE and Western blotting. Results and Conclusion The recombinant plasmid P^PICZαA-Ts87 has been constructed. Ts87 gene has been expressed and secreted in Pichia pastoris GS115 strain.

Key words: Trichinella spiralis, Helminth genes, Pichia pastoris, Gene expression