Abstract: 　Objective To ascertain the changes of haemolymph protein concentration in adult Anopheles stephensi mosquitoes under different feeding conditions. Methods Haemolymph samples from four groups of adult An.stephensi, fed with sucrose solution, normal blood, plasmodium-infected blood and nitroquine, respectively, were collected by expulsion method. The concentration of haemolymph protein was examined by Bradford method. The results were analyzed automatically by Excel program. Results The level of protein concentration in the infected blood-fed group was higher than the sucrose solution group and normal blood group at day 8 after Plasmodium yoelii infection,the average concentration was 4.436,3.080 and 3.092 μg/μl,respectively. The haemolymph protein concentration (2.264 μg/μl) in the nitroquine-administered mosquitoes was lower than the infected blood-fed mosquitoes. Conclusion The haemolymph protein concentration of the adult An. stephensi decreases after the nitroquine administration, indicating that the haemolymph proteins may be involved in the melantotic encapsulation reaction of plasmodial oocysts.

Key words: haemolymph protein, Anopheles stephensi, Plasmodium yoelii