›› 2001, Vol. 19 ›› Issue (3): 10-168.

• 实验报道 • Previous Articles     Next Articles

Studies on Immunological Reaction of the Antiserum of Recombinant Secreted Protein from Ancylostoma Caninum

WEN Li-yong1;Hotez PJ2   

  1. 1 Institute of Parasitic Diseases;Zhejiang Academy of Medical Sciences*;Hangzhou 310013; 2 Yale University School of Medicine;Connecticut
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-06-30 Published:2001-06-30

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Abstract: 　Objective To analyze the immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum with antigens of various species hookworms at different developmental stages. Methods SDS-PAGE and ELIB technique were employed in the study. Results and Conclusion The protein component of Ac-rAsp-1 was 45 kDa, its immune serum can recognize the antigens of Ac-L\-3 and Ac-rAsp-1 protein, but not react to the antigens of Ad-A?Ad-L\-3?Na-A?Ac-A?Nb-A and Ac-rAsp-2 protein. The protein component of Ac-rAsp-2 was 24 kDa, its immune serum can recognize the antigens of Ad-A?Ad-L\-3?Na-A?Ac-A?Ac-L\-3 and Ac-rAsp-2 protein, but not react to the antigens of Nb-A and Ac-rAsp-1 protein.

Key words: recombinant secreted protein from Ancylostoma caninum, hookworm, immunological reactivity