›› 2001, Vol. 19 ›› Issue (2): 2-71.

• 论著 • Previous Articles     Next Articles

Screening and Sequence Analysis of the Adult Stage-specific Gene of Trichinella spiralis

YANG Jing 1;ZHU Xin ping 1*;ZHANG Xin mei 1;YANG Ya ping 1 ZHOU Lei 1;GAO Xin 1;LIU Ming yuan 2;Boireau Pascal 3   

  1. 1 Department of Parasitology;Capital University of Medical Sciences;Beijing 100054; 2 Trichinellosis Lab;the
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-04-30 Published:2001-04-30

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Abstract: 　Objective To obtain the sequence of the adult stage specific gene of Trichinella spiralis . Methods An adult T. spiralis cDNA library was screened using an adult T. spiralis stage specific probe. The gene sequence was analyzed by DNAstar software and GenBank database. Results A cDNA clone of 1 629 bp was obtained by screening the library with an adult stage specific probe. The clone contained one open reading frame composed of 1 464 bp encoding 487 amino acids. GenBank searching revealed that no identical cDNA was found. Two zinc fingers located at the positions within amino acids 207-270. Some possible antigen epitopes were predicted within the amino acids 52-64,108-116,137-163 and 226-260. Conclusion A novel stage specific gene of adult T. spiralis encoding the protein containing certain predicted antigen epitopes was obtained.

Key words: Trichinella spiralis, adult cDNA library, cDNA clone, sequence analysis subtracted hybridization