Abstract: 　AIM: : To determine the protective effect of leishmanial surface antigens against experimental visceral leishmaniasis in Lagurus lagurus caused by Leishmania infantum .METHODS: Recombinant surface protein (rGP63) and lipophosphoglycan (LPG) of Leishmania were used with Corinebacterium parum vaccine as ajuvant to immunize Lagurus lagurus against a challenge with virulent strain of Leishmania infantum . The efficacy of immunoprotection was observed.RESULTS: When challenged with up to 2×10 7 promastigotes, the number of LD on the liver printing sections in the rGP63+LPG+CP immunised animals was significantly decreased ,the parasite reduction rate being 89.79%. LPG+CP gave a parasite reduction rate of 60.6% and rGP63/β-galactosidase fusion protein +CP showed a parasite reduction rate of 42.45%.Purified rGP63 showed no protection. Immunization with rGP63+LPG+CP followed by challenge inifection with 1×10 6,5×10 6 and 1×10 7 promastigotes also showed significantly reduced infection rates.CONCLUSION: A combination of rGP63+LPG+CP antigens could provide significant immunoprotection against L.infantum challenge in L.lagurus .

Key words: GP63, LPG, immunoprotection, Lagurus lagurus Pallas, visceral leishmaniasis, Leishmania infantum