›› 1999, Vol. 17 ›› Issue (2): 11-105.

• 论著 • Previous Articles     Next Articles

STUDIES ON IMMUNOPROTECTION OF MONOCLONAL ANTIBODIES AGAINST CRYPTOSPORIDIUM PARVUM

ZONG Haihong 1;LI Weiming 1;ZHANG Xue 2;YU Shurong 2;BAI Banjun 1

  

  1. 1　Department of Pediatrics, Xinqiao Hospital, Third Military Medical U niversity , Chong qing 4100372　Department of microorganism , Third Military Medical University , Chong qing 410037
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-04-30 Published:1999-04-30

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Abstract: 　AIM: To explore the immune protection of the monoclonal antibody (McAb) againstCryptosporidium parvum.METHODS: On the basis of Madin Darby canine kidney (MDCK) cell culture, the immunoprotection of McAb against C.parvum was screened by means of neutralization test in vitro, and verified by rat model and transmission electron microscopy of infected MDCK cells. RESULTS: The number of C.parvum sporozoites recovered on the surface of rat intestinal epithelial cells and the mean output of oocyst were significantly reduced by McAb Z3D2 (P<0 001, P<0 05). The decreased Cryptosporidium at each development phase within infected MDCK cells and less damage of cell ultrastructure caused by Cryptosporidium were found in Z3D2 treated group. CONCLUSION: McAb Z3D2 possesses highly effective protection against C.parvum.

Key words: Cryptosporidium parvum, Madin Darby canine kidney cells, McAb, immunoprotection