›› 1998, Vol. 16 ›› Issue (6): 432-435.

• 论著 • Previous Articles     Next Articles

ANALYSIS OF NUCLEAR DNA GENE TYPES OF LEISHMANIA ISOLATES FROM HILLY AND PLAIN FOCI OF CHINA

Lu Fangli 1; Hu Xiaosu 1; Jing Baoqian 2; Ma Ying 1   

  1. 1 Department of Parasitology; West China University of Medical Sciences; Chengdu 610041 2 Department of Microbiology; North Sichuan Medical College; Nanchong 637000
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-12-31 Published:1998-12-31

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Abstract: AIM: To analyse the nuclear DNA (nDNA) polymorphism of Leishmania isolates from hilly and plain foci of China. METHODS: nDNA were analysed by endonuclease digestion, Southern blotting and chromosomal localization. Probes were labeled with digoxigenin. RESULTS: Using gp63 gene probe, similar hybridization bands were found to be existed between nDNAs of Leishmania donovani Jiangsu human isolate and L.d.Jeddah, also between nDNAs of L.d. Sichuan human isolate and L.infantum. Using β-tubulin gene probe, there were two similar hybridization bands existed between nDNAs of L. d. Jiangsu human isolate and L. d. Jeddah, and three similar hybridization bands existed between nDNAs of L. d. Sichuan canine isolate and L. d. Gansu canine isolate, and two similar hybridization bands existed between nDNA of L. d. Sichuan canine isolate and L. d. Wenchuan human isolate. CONCLUSION: Homology exists between L. d. Jiangsu human isolate and L. d. Jeddah from plain foci, between L. d. Sichuan human isolate from hilly foci and L. infantum, between L. d. Sichuan canine isolate and L. d. Gansu canine isolate from hilly foci. Homology
as well as differences exist between L. d. Sichuan canine isolate and L. d. Wenchuan human
isolate. Heterogeneity exists between isolates from hilly foci and plain foci.

Key words: Leishmania, nDNA, Southern blotting, hilly foci, plain foci, homology, heterogeneity