›› 1997, Vol. 15 ›› Issue (6): 392-394.

• 实验报道 • Previous Articles     Next Articles

IN VITRO CULTIVATION OF THE GERMINAL CELLSFROM LARVAL ECHINOCOCCUS GRANULOSUS

Lu Jiahai1; Cheng Weixing1; Guo Zhongmin2; Feng Deyuan1; Chen Qijun3; Li Dechang3; Guo Gu2   

  1. 1 Urumqi General Hospital of Lanzhou Command; Urumqi 830000 2 Xinjiang Academy of Animal Science; Urumqi 830000 3 University of Agriculture and Anima Science, PLA, Chang chun 130062
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

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Abstract: AIM:To establish a cell line of larval Echinococcus granulosus. METHODS:The germinal cells and protoscoleces were obtained surgically from the patient with hydatidosis.The materials of germinal layer and protoscoleces were perpared for primary cultures. Trypsin was used for the enzymatic release of the monodispersed cells from the protoscoleces. The culture media were RPMI1640, 199 and improved DMEM with 15% - 20% fetal calf serum. RESULTS:Using improved DMEM medium and rat- tail collagen- coated flasks were suitable for cells on growth of larval Echinococcus granulosus, and was established cell line successfully. It has been 20 passages up to now. The times of primary cultures needed 28- 45 day. The cultured cells at passage 1- 3 were varying in size and shape. CONCLUSION: A method was established for culture cells of larval Echinococcus granulosus from patient.

Key words: Larval Echinococcus granulosus, in vitro cell culture, cell line