›› 1997, Vol. 15 ›› Issue (6): 353-355.

• 论著 • Previous Articles     Next Articles

STUDIESON THE SANDWICH DOT-ELISA IN DETECTING SCHISTOSOME ANTIGEN IN ONCOMELANIA SNAILS

Shen Dakang1; Lou Wenxian1; Zheng Zhiguo2   

  1. 1 Departmentof Parasitology; Shanghai Second Medical University; Shanghai 200025 2 Zhejiang Provincial Institute of Parasitic Disease; Hangzhou 310013
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

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Abstract: AIM:To observe the feasibility and reliability of sandwich dot- ELISA in detecting schistosome antigen in Oncomelania snails. METHODS:Combined monoclonal antibodies were used in detecting schistosome antigen of Oncomelania snails which were not infected or infected differently with miracidia. RESULTS:Compared with the results of microscopy, both the positive rate and correspondent rate rise gradually within 1-3 weeks in the group which was infected with collective miracidia, the positive rate, sensitivity and correspondent
rate reached 100% during 4-9 weeks except week 6, being almost the same as the results of microscopy; All the results of the uninfected group were negtive, just the same as microscopy. The results of Oncomelania snails which infected with other trematodes were negative in dot-ELISA. CONCLUSION: Sandwich dot-ELISA might be used to detect schistosome antigen in Oncomelania snails.

Key words: Sandwich dot- ELISA, Oncomelania, schistosome antigen, combined monoclonal antibodies