›› 1997, Vol. 15 ›› Issue (6): 335-339.

• 论著 • Previous Articles     Next Articles

OLE OF NITRIC OXIDE PRODUCED BY IFN-γ-ACTIVATED MACROPHAGECSIN THE KILLING OF MALARIA PARASITE

Niu Yuxin1; L i Huizhu2; Zhang Haiyan3; Wang Fengyun2   

  1. 1 Departmentof Cytobiology and Genetics; Capital University of Medical Sciences; Beijing100054 2 Department of parasitology, Capital University of Medical Sciences, Beijing 1000543 Department of Cytobiology, Capital University of Medical Sciences, Beijing 100054
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

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Abstract: AIM:To explore the role of nitric oxide (NO) produced by IFN- γ- activated macrophages
in the killing of malaria parasite.METHODS:After the isolated murine peritonal macrophages were
inoculated with IFN- γ of different concentrations for48h, P.yoelii parasitized erytherocytes(PRBCs)
were added for another4h.For in vivo studies mice were infected ip with107 PRBCs,NO produced by IFN-
γ- activated- macrophages both in vitro and in vivo were measured and the cytotoxicity of macrophage
on Plasmodium yoelii parasites was examinded. RESULTS: A positive correlation was found between the cytotoxicity of macrophages and NO levels ( r= 0.898, P < 0.01). L-NNA, an inducible NO synthase (iN-OS) competitive inhibitor, could inhibit the production of NO by macrophages and reduced the cytotoxicity ofmacrophages cytotoxicity on P. yoelii. IFN- γ was found to protect BALB/c mice against P. yoelii infection in vivo, while in jection of L-NNA resulted in high level of parasitemia and increased mortality in infected mice. CONCLUSION: The plasmodicidal cytotoxicity could be attributed to the nitric oxide produced by the IFN -γ-activated macrophages.

Key words: Interferon- γ, macrophage s cytotoxicity, nitric oxide, Plasmodium yoelii, parasitemi