PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST ESTERASE OF MALATHION RESISTANT CULEX QUINQUEFASCIATUS

›› 1995, Vol. 13 ›› Issue (1): 46-49.

PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST ESTERASE OF MALATHION RESISTANT CULEX QUINQUEFASCIATUS

Online:1995-02-28 Published:2017-01-05

Abstract

Abstract: Five clones of monoclonal antibodies (McAbs) were generated from BALB/c mice which were activized with PPD and immunized with esterases extracted from malathion resistant Culex quinquefasciatus . All 5 clones of McAbs belonged to the IgG 1 subtype, additive index (A.I.) of every two McAbs did not exceeded 50%. After a l h of incubation, the McAbs at the titer of 1∶1 000 completely inhibited the activity of the amplified esterases. Of 8 strains of adult mosquitoes studied by Western blot assay, all exhibited the 64 kDa esterase band recognized by the McAbs, six (SP,FS,SH,CD,RD,RM) showed more bands of different MW (43-60 kDa), the higher the organophosphorus resistant level, the more and the denser the bands. The above McAbs had little or no effect against the esterases of dimethrin resistant strain and the larva stage of malathion resistant Culex quinquefasciatus .

CLC Number:

R392-33

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PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST ESTERASE OF MALATHION RESISTANT CULEX QUINQUEFASCIATUS

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