Abstract: Serum tests were made on 116 confirmed cases of clonorchiasis, 103(88.8%) of them showed positive, while only 6 (4.4%) were positive among 138 healthy people. Samples were collected on filter paper stripes. 111 (95.7%) cases were positive among 116 confirmed cases tested, but only 2 (1.5%) were positive out of 138 healthy persons. The results were similar to those obtained by sheep anti-human IgG.Animal experiment also showed that the SPA-ELISA can be used for the diagnosis of clonorchiasis.In an endemic area, stool positive rate was 8.8% (62/703). When checked with SPA-ELISA, the rate of conformity in both filter paper stripes and stool examinations was 90.3% (56/62). Among 641 serum tests from individuals negative in stool examinations, only 35 (5.5%) reacted positively. The authors suggested that SPA-ELISA with soluble clonorchis antigens could be used in a large scale seroepidemiological survey in endemic areas.