Frequency of I1532T mutations in the voltage-gated sodium channel of Aedes albopictus in Guangyuan City

doi:10.12140/j.issn.1000-7423.2026.03.019

Abstract

Abstract:

To investigate the frequency of the I1532T mutation in the voltage-gated sodium channel (vgsc) of Aedes albopictus in Guangyuan City, Sichuan Province, adult Ae. albopictus mosquitoes were collected using CO₂-baited light trapsfrom residential areas in seven counties (districts) of Guangyuan City between June and July in 2024. Genomic DNA was extracted from mosquitoes, and the genotype at the vgsc 1532 locus was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The detection performance of PCR-RFLP assay was validated by sequencing. Hardy-Weinberg equilibrium (HWE) test for populations was assessed using the software Genepop 4.7, and Fisher’s exact test was used to compare allele frequencies across counties (districts) using the software SPSS 25. A total of 208 Ae. albopictus mosquitoes were detected. PCR amplification of all samples showed bands with approximately 220 bp in length, which was consistent with the expected size. The frequencies of the vgsc wild-type homozygous 1532I/I genotype, wild-type/mutant heterozygous 1532I/T genotype, and resistant homozygous 1532T/T genotype were 87.98% (183/208), 10.58% (22/208), and 1.44% (3/208) in Guangyuan City, respectively. Among 7 counties (districts), the frequencies of wild-type/mutant heterozygous 1532I/T genotype were 12.5% (4/32), 9.38% (3/32), 17.86% (5/28), 28.13% (9/32) and 1/16 in Jiange County, Lizhou District, Qingchuan County, Wangcang County and Zhaohua District, respectively, while no heterozygous genotype were found in Chaotian District or Cangxi County. The frequencies of resistant homozygous 1532T/T genotype were 6.25% (2/32) and 3.13% (1/32) in Lizhou District and Wangcang County, respectively, and this homozygous genotype were not detected in other counties (districts). The frequencies of the vgsc wild-type 1532I and mutant 1532T alleles were 93.27% (388/416) and 6.73% (28/416) in Guangyuan City, respectively, and the frequencies of mutant 1532T alleles were 0 (0/64), 0 (0/72), 6.25% (4/64), 10.94% (7/64), 8.93% (5/56), 17.19% (11/64), and 3.13% (1/32) in Chaotian District, Cangxi County, Jiange County, Lizhou District, Qingchuan County, Wangcang County, and Zhaohua District, respectively (P < 0.05). HWE test showed that the Ae. albopictus population in Lizhou District deviated significantly from equilibrium (P < 0.05), and Ae. albopictus populations from Jiange County, Qingchuan County, and Wangcang County were at equilibrium (P > 0.05), while Ae. albopictus populations from other three counties (districts) were not suitable for HWE tests due to absence of the 1532T mutation (Chaotian District and Cangxi County) or low mutation frequency (Zhaohua District). The verification results showed that the coincidence rate between PCR-RFLP and sanger sequencing results was 100%. This study indicates a relatively high frequency of the vgsc I1532T mutation in Ae. albopictus populations from Guangyuan City, with resistant homozygotes detected in Lizhou District and Wangcang County.

Key words: Aedes albopictus, Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), Voltage-gated sodium channel, Knockdown resistance mutation

CLC Number:

R384.1

LU Xiaoqiang, YANG Binyu, ZHAO Qiongyao, KONG Xinming, LIU Yanchun, JIA Yongchao. Frequency of I1532T mutations in the voltage-gated sodium channel of Aedes albopictus in Guangyuan City[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2026, 44(3): 443-447.

Figures/Tables 2

References 22

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区（县）	数量	基因型蚊虫数（频率/%）
区（县）	数量	野生型I/I	野生型/突变型I/T	抗性T/T
朝天区	32	32（100）	0（0）	0（0）
苍溪县	36	36（100）	0（0）	0（0）
剑阁县	32	28（87.50）	4（12.50）	0（0）
利州区	32	27（84.38）	3（9.38）	2（6.25）
青川县	28	23（82.14）	5（17.86）	0（0）
旺苍县	32	22（68.75）	9（28.13）	1（3.13）
昭化区	16	15（15/16）	1（1/16）	0（0）
合计	208	183（87.98）	22（10.58）	3（1.44）