Epidemiological investigation of paragonimiasis epidemic focus in Luoyang City of Henan Province

doi:10.12140/j.issn.1000-7423.2020.05.023

Abstract

Abstract:

To investigate the status of Paragonimus spp. infection in freshwater crabs in Luoyang City of Henan Province, and identify the epidemic focus of paragonimiasis. Freshwater crabs as the second intermediate host were collected from surveillance sites in 9 counties(cities) of Luoyang City during the time from August 2013 to October 2019. Paragonimus spp. infection in the crabs was detected in laboratory settings. Paragonimus spp. metacercariae were identified by PCR amplification of the sequence of internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome oxidase subunit 1(CO1). A total of 560 freshwater crabs were captured, and Paragonimus metacercariae were found in the crabs from Yiyang County, Luoning County, Xin’an County, Song County, and Luanchuan County, and identified as the species P. skrjabini by morphological observation and PCR amplification. The infection rate and infectivity were found highest in the crabs collected from Yiyang County (58.33%, 11.54 metacercariae/crab), followed by Xin’an County (25.93%, 0.33 metacercariae/crab), Song County (19.64%, 0.23 metacercariae/crab), Luoning County (11.96%, 0.12 metacercariae/crab), and Luanchuan County (6.17%, 0.06 metacercariae/crab). Results of PCR showed that the ITS2 amplification product has a sequence length of 500 bp from the metacercariae isolated from the crabs of Yiyang County, Xin’an County, Songxian County, Luoning County and Luanchuan County, with 98%-100% homology to P. skrjabini (GenBank accession No. KX129924.1). It was recognized that five counties including Yiyang County, Luoning County, Xin’an County, Luanchuan County, and Song County were identified to be the epidemic foci of paragonimiasis among them, Yiyang County was a moderate endemic area, while the others being mild endemic regions.

Key words: Paragonimus, Freshwater crabs, Endemic focus, Investigation, Luoyang

CLC Number:

R532.22

LI Yun-xia, AI Lin, DENG Yan, CHEN Wei-qi, ZHU Xin, YANG Zhi-guo, LI Ke-wei, SUN Yi-ying. Epidemiological investigation of paragonimiasis epidemic focus in Luoyang City of Henan Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(5): 664-666.

Figures/Tables 2

References 16

[1]	Blair D. Paragonimiasis[J]. Adv Exp Med Biol, 2019,1154:105-138. doi: 10.1007/978-3-030-18616-6_5 pmid: 31297761
[2]	Zhu XP, Su C. Human parasitology[M]. Beijing: People’s Medical Publishing House, 2013. (in Chinese)
	( 诸欣平, 苏川. 人体寄生虫学[M]. 北京: 人民卫生出版社, 2013.)
[3]	Oh MY, Chu A, Park JH, et al. Simultaneous Paragonimus infection involving the breast and lung: A case report[J]. World J Clin Cases, 2019,7(24):4292-4298. doi: 10.12998/wjcc.v7.i24.4292 pmid: 31911910
[4]	Yoshida A, Doanh PN, Maruyama H. Paragonimus and paragonimiasis in Asia: an update[J]. Acta Trop, 2019,199:105074. doi: 10.1016/j.actatropica.2019.105074 pmid: 31295431
[5]	World Health Organization. Investing to overcome the global impact of neglected tropical disease: third WHO report on neglected tropical disease[R]. WHO: Geneva, 2015.
[6]	Wang LD. A national survey on current status of the important parasitic diseases in human population[M]. Beijing: People’s Medical Publishing House, 2008. (in Chinese)
	( 王陇德. 全国人体重要寄生虫病现状调查[M]. 北京: 人民卫生出版社, 2008.)
[7]	Zhou XN. Report on the national survey of important human parasitic diseases in China in 2015[M]. Beijing: People’s Medical Publishing House, 2018. (in Chinese)
	( 周晓农. 2015年全国人体重点寄生虫病现状调查报告[M]. 北京: 人民卫生出版社, 2018.)
[8]	Chen GX, Chang J, Zhang XQ, et al. A preliminary investigation of freshwater crabs as the second intermediate host of Paragonimus in Henan Province[J]. Chin J Zool, 1982(4):1-3. (in Chinese)
	( 陈国孝, 常江, 张秀清, 等. 河南省肺吸虫第二中间宿主淡水蟹类的初步调查[J]. 动物学杂志, 1982(4):1-3.)
[9]	Guo ZY, Liu Y. 36 patients with paragonimiasis investigation report[J]. Henan J Prev Med, 2013,24(3):217-219. (in Chinese)
	( 郭正印, 刘英. 36例肺吸虫病调查报告[J]. 河南预防医学杂志, 2013,24(3):217-219.)
[10]	Wei HW, Cheng YZ, Zeng Y, et al. A survey of the new epidemic area of Paragonimus skriabini in Zhenghe, northern Fujian[J]. J Trop Med, 2010,10(1):83-84, 89. (in Chinese)
	( 魏焕旺, 程由注, 曾永平, 等. 福建北部政和县斯氏并殖吸虫病新疫区调查[J]. 热带医学杂志, 2010,10(1):83-84, 89.)
[11]	Dai AY, Song YZ. Description of new species of freshwater crabs as intermediate hosts of Paragonimus and other flukes in China[J]. J zool, 1975,21(3):261-262. (in Chinese)
	( 戴爱云, 宋玉枝. 我国肺吸虫及他种吸虫中间宿主——淡水蟹类新种描述[J]. 动物学报, 1975,21(3):261-262.)
[12]	Sheng YP, Zhang YJ. Preliminary observation on the biology and protein bands of Paragonimus sanping[J]. Chin J Parasitol Parasit Dis, 1987,5(4):278-280. (in Chinese)
	( 沈一平, 张耀娟. 三平正并殖吸虫囊蚴的生物学与蛋白质区带初步观察[J]. 中国寄生虫学与寄生虫病杂志, 1987,5(4):278-280.)
[13]	Wu ZD, Huang Y, Song LG. New challenge for human parasitic disease control in China: food-borne parasitic disease control[J]. China Trop Med, 2019,19(1):1-3. (in Chinese)
	( 吴忠道, 黄艳, 宋兰桂. 我国人体寄生虫病防治的新挑战: 食源性寄生虫病的防治[J]. 中国热带医学, 2019,19(1):1-3.)
[14]	Chen JX. Food-borne parasitic disease[M]. Beijing: People’s Medical Publishing House, 2009. (in Chinese)
	( 陈家旭. 食源性寄生虫病[M]. 北京: 人民卫生出版社, 2009.)
[15]	Ma YX, Shang LY, Xu BL. Analysis and prevention of parasitic diseases in Henan Province[M]. Zhengzhou: Henan People’s Publishing House, 2014. (in Chinese)
	( 马云祥, 尚乐园, 许汴利. 河南省寄生虫病防治与研究[M]. 郑州: 河南人民出版社, 2014.)
[16]	Chen W, Deng Y, Zhang YL, et al. A case of group infections with Paraginimus species in Henan, Central China[J]. Acta Trop, 2020,202:105111. doi: 10.1016/j.actatropica.2019.105111 pmid: 31351073