DNA条形码技术在蚊虫分类鉴定中的研究进展

摘要/Abstract

摘要：

DNA条形码在物种分类鉴定中具有快速、准确的特点。近年来,基于线粒体细胞色素c氧化酶亚基Ⅰ（cytochrome c oxidase subunit Ⅰ,COⅠ）基因的DNA条形码技术已成功应用于蚊虫物种水平的鉴定。本文主要对DNA条形码技术及原理,基于COⅠ基因的DNA条形码技术对蚊虫种和种团鉴定的应用及其局限性,以及基于COⅡ、16S RNA、核糖体DNA内转录间隔区（ribosome DNA internal transcribed spacer,rDNA-ITS）中的第一内转录间隔区（the first internal transcribed spacer,ITS1）和第二内转录间隔区（the second internal transcribed spacer,ITS2）等其他分子标记在蚊虫分类鉴定中的应用及其辅助COⅠ基因对蚊虫进行分类鉴定的研究进展进行综述。

关键词: DNA条形码, 分类鉴定, 蚊虫, 分子标记

Abstract:

DNA barcoding technique is a fast and accurate method for species identification. The DNA barcoding based on cytochrome c oxidase subunit Ⅰ (COⅠ) has recently been successfully applied for species identification of Culicidae. In this paper, we introduce the technique and principle of DNA barcoding, application and limitation of the technique based on CO I gene for species identification, as well as research development on applications of other molecular makers such as COⅡ, 16S RNA, and the first and the second internal transcribed spacers (ITS1 and ITS2) in species identification and to assist the COⅠ gene in identifying Culicidae species.

Key words: DNA barcode, Taxonomy, Culicidae, Molecular marker

中图分类号:

R384.11

郭玉燕, 罗雷, 郑学礼. DNA条形码技术在蚊虫分类鉴定中的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(1): 93-98.

Yu-yan GUO, Lei LUO, Xue-li ZHENG. Research progress on application of DNA barcoding technique in Culicidae taxonomy[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2017, 35(1): 93-98.

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