关键词: 牛带绦虫, 诊断, 治疗, 细胞色素C氧化酶1基因

Abstract:

Objective To diagnose and provide treatment for a local case of taenia infection in Zhejiang Province and identify the species of the worm. Methods The information of disease onset, clinical feature and therapeutic process was collected and epidemiological investigation was carried out. The anal cellophane swab was used to detect the eggs. Areca and pumpkin seeds were used for deworming. Morphological observation, PCR amplification and sequencing of cytochrome C oxidase 1 （COX1） gene were performed for the discharged worm. Results The epidemiological results showed that the patient did not go outside Pujiang County in the past two years, and had no history of eating raw pork, beef or animal offal. But she often had barbecues and hot-pot food, occasionally with raw vegetables. Taenia eggs were found on her perianal skin. The discharged worm was suspected to be Taenia saginata or Taenia asiatica by morphological observation. PCR amplification of COX1 resulted in a band of 832 bp, which was 99%, 96% and 88% homologous to COX1 of Taenia saginata （GenBank accession number: AB107239.1）, Taenia asiatica（GenBank accession number: AB107235.1） and Taenia solium（GenBank accession number: AB066485.1）, respectively. Conclusion According to the clinical feature, epidemiological information and sequencing results, this case is confirmed to be a local infection of Taenia saginata.

Key words: Taenia saginata, Diagnosis, Treatment, cytochrome C oxidase 1 gene