关键词: 刚地弓形虫, 人脐静脉内皮细胞, 小鼠单核巨噬样细胞, 细胞骨架, Ca²⁺

Abstract: Objective To explore the change of cytoskeleton and the variance of Ca²⁺ in cultured cells during the invasion of Toxoplasma gondii. Methods Tachyzoites suspensions were gathered by routine method and used to infect phagocytic cells（J774A.1）and non-phagocytic cells （HUVEC）. The ability of T. gondii invading into the cells and the influence of cytoskeleton inhibitor，colchicine and cytochalasin D，were observed by microscopy. The rearrangement of cytoskeleton of cells was observed by fluoromicroscopy. By using laser scanning confocal microscope，the variance of Ca²⁺ in J774A.1 and HUVEC was detected. Results Ca²⁺ increased greatly in J774A.1 during the invasion of T.gondii（P<0.01）and PLC inhibitor，U73122，could block the increase of Ca²⁺（P>0.05）. The microfilaments of J774A.1 were agglomerated during the invasion of T. gondii. Cytoskeleton inhibitor，cytochalasin D（P<0.01）and colchicine（P<0.05）significantly reduced the infection rate of J774A.1 cells. No considerable change of Ca²⁺ in HUVEC was found （P>0.05）during the invasion and cytoskeleton was not changed. Cytochalasin D and colchicine showed little effect on the infection rate of HUVEC. Conclusion The concentration of Ca²⁺ increases greatly and cytoskeleton（mainly the microfilament）has been rearranged in phagocytic cell during the invasion of T. gondii，while both of them show no significant change in non-phagocytic cell.

Key words: Toxoplasma gondii, HUVEC, J774A.1, Cytoskeleton, Ca²⁺