旋毛虫在小鼠先天性传播的初步研究

中国寄生虫学与寄生虫病杂志 ›› 2005, Vol. 23 ›› Issue (2): 3-77.

旋毛虫在小鼠先天性传播的初步研究

王中全;韩化敏;崔晶

郑州大学医学院寄生虫学教研室，郑州 450052

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-04-30 发布日期:2005-04-30

Preliminary Study on Congenital Transmission of Trichinella spiralis in Mice

WANG Zhong-quan;HAN Hua-min;CUI Jing

Department of Parasitology, Medical College of Zhengzhou University, Zhengzhou 450052, China

Received:1900-01-01 Revised:1900-01-01 Online:2005-04-30 Published:2005-04-30

摘要/Abstract

摘要： 目的?摇研究旋毛虫在小鼠的先天性传播并观察母鼠抗旋毛虫抗体对攻击感染的保护作用。方法将昆明小鼠分为受孕后感染组和感染后受孕组，子鼠出生后1 d内剖杀，检查旋毛虫幼虫；将正常母鼠所产子鼠由感染旋毛虫的母鼠喂养，21 d后宰杀，检查旋毛虫幼虫。用间接ELISA检测感染母鼠所产子鼠出生后不同时间的血清抗旋毛虫抗体，观察母鼠抗旋毛虫抗体对攻击感染的免疫保护。结果受孕后7 d感染旋毛虫的母鼠所产的6只子鼠中有2只感染旋毛虫；感染旋毛虫后8 d和22 d受孕雌鼠所产子鼠的感染率分别为20%（2/10）和25%(2/8)，从子鼠检获的旋毛虫均是未成囊的幼虫。交叉哺乳实验表明正常母鼠所产的30只子鼠未见旋毛虫感染。感染母鼠所产27只子鼠出生后1、7、24及40 d的血清抗体阳性率分别为100%、100%、77.8%及14.8%，子鼠出生后40 d攻击感染的减虫率为62.0%；感染母鼠所产子鼠血清被动转移小鼠的减虫率55.7%，与对照组比较差异有显著性（P<0.01）。结论旋毛虫在小鼠可经胎盘传播，母鼠的抗旋毛虫抗体对子鼠抗攻击感染可能具有部分保护作用。

关键词: 旋毛虫, 先天性传播, 交叉哺乳, 免疫保护

Abstract: Objective To study congenital transmission of Trichinella spiralis in mice and observe the protection of anti-Trichinella antibodies from the infected dams to challenge infection. Methods According to the gestation (fertilization), the Kunming mice were divided into two groups: the infected group after gestation and the gestated group after infection. New-born mice were cut into small pieces to separate the larvae within 1 day after birth. One-day-old offspring born to normal dams were nursed by the infected dams, slaughtered after 21 days and examined for the larvae. Serum anti-Trichinella antibody level in offspring born to the infected dams was assayed by ELISA at different time after birth, and its immune protection against challenge infection was studied. Results Out of 6 offspring born to the dams infected at 7 days after fertilization, two were found to be infected. Among other female mice which were first infected with T. spiralis and then gestated, only the offspring born to the dams fertilized at 8 and 22 days after infection were found to be infected, the infection rate of offspring was 20% (2/10) and 25%(2/8) respectively. All larvae recovered from the young were non-encapsulated. The cross-fostering experiment showed that none of 30 offspring born to normal dams were found to be infected. The serum antibody positive rate in 27 offspring born to the infected dams at 1, 7, 24, and 40 days after birth was 100%, 100%, 77.8% and 14.8%, respectively. The worm reduction rate in the offspring 40 days after birth was 62.0% after challenge infection. The worm reduction rate in mice in which sera from the offspring born to the infected dams were passively transferred was 55.7%, there was a significant difference (P<0.01) compared to the control group. Conclusion A transplacental transmission of T. spiralis is revealed in mice. Anti-Trichinella antibodies from the infected dams may partially protect the young from challenge infection.

Key words: Trichinella spiralis, Trichinella spiralisCongenital transmission, Cross-fostering, Immune protection

王中全;韩化敏;崔晶. 旋毛虫在小鼠先天性传播的初步研究[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(2): 3-77.

WANGZhong-quan;HANHua-min;CUIJing. Preliminary Study on Congenital Transmission of Trichinella spiralis in Mice[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2005, 23(2): 3-77.

[1]	姜文静, 孟雅莉, 赵利娜, 王春苗, 张晓磊. 刚地弓形虫棒状体蛋白18和膜表面抗原30复合核酸疫苗对小鼠的免疫保护作用[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 532-538.
[2]	张旭, 孙希萌. 旋毛虫感染免疫逃逸机制研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 492-496.
[3]	郝会囡, 程永康, 张茹, 韩璐璐, 宋艳艳, 龙绍蓉, 刘若丹, 张玺, 王中全, 崔晶. 旋毛虫新生幼虫可溶性抗原的免疫蛋白组学分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 176-182.
[4]	蔡子涵, 曹颖, 朱逢龙, 李倩, 和艳红, 杨毅梅. 纳米金棒标记技术应用于旋毛虫感染诊断的研究[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(5): 652-658.
[5]	王国英, 陈丹丹, 郑学礼, 李祥会, 张浩, 张军, 滕铁山. 旋毛虫成囊幼虫的感染性与其发育阶段的关系[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(6): 785-788.
[6]	谭潇, 肖楚丽, 肖非, 王硕, 卿蕊, 黄泽智. IL-18增强pcDNA3.1/SjOST48抗血吸虫感染的免疫保护性研究[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(3): 279-285.
[7]	王婵, 杨松昊, 董飞, 杜先才, 杨继辉, 牛楠, 朱明星, 王浩, 王娅娜, 赵巍. LncRNA 017418及细胞因子在细粒棘球蚴rP29免疫小鼠中的表达分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(2): 201-206.
[8]	张雨璐, 王洋, 白雪, 唐斌, 胡晓祥, 张春玲, 刘明远, 刘晓雷. 旋毛虫和伪旋毛虫肌幼虫时期排泄分泌产物iTRAQ法蛋白质组学分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1): 47-53.
[9]	王国英, 李祥会. 旋毛虫肌幼虫在小鼠体内发育的实验观察[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(2): 235-237.
[10]	魏玉环, 胡媛, 曹建平. 抗细粒棘球绦虫疫苗的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(1): 97-101.
[11]	李徽徽, 贺文欣, 蔡兆根, 仇大鹏, 褚亮, 陈兴智, 方强, 夏惠, 李楠, 崔宁宁, 徐岚松, 杨小迪. 旋毛虫成虫排泄-分泌蛋白对脓毒症急性肝损伤的保护作用及机制研究[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(2): 99-104.
[12]	杨小迪, 李徽徽, 陶志勇, 方强, 程洋, 徐岚松, 薛仁敏, 陈勇, 夏惠, 张慧, 姜辉, 刘涛, 彭琨, 陈兴智. 两种蠕虫半胱氨酸蛋白酶抑制剂对小鼠腹腔渗出细胞一氧化氮产生及细胞因子分泌的影响[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(2): 110-114.
[13]	杨小迪, 陶志勇, 程洋, 吴琦, 王小莉, 宋迪, 徐岚松, 薛仁敏, 常雪莲, 张慧, 汪瑞, 陈兴智, 方强. 旋毛虫成虫排泄分泌蛋白组分分析[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(1): 24-29.
[14]	丁忆晗，王小莉，宋迪，吴琦，杨小迪*. 旋毛虫及其衍生产物调节过敏性及自身免疫性疾病的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(4): 17-382-386.
[15]	刘博宇，王丞，邢鑫，陈宏亮，姜晶，蔡亚南，王春凤，杨桂连*. 旋毛虫感染小鼠树突状细胞相关C型凝集素-2动态变化[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(2): 3-105-108.