中国寄生虫学与寄生虫病杂志 ›› 2001, Vol. 19 ›› Issue (3): 11-172.

• 实验报道 • 上一篇    下一篇

人芽囊原虫的形态与超微结构

何妮;张月清;洪明理;丛敏
  

  1. 北京热带医学研究所!北京100050(何妮;张月清);首都医科大学附属北京友谊医院实验中心!北京100050(洪明理;丛敏)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-06-30 发布日期:2001-06-30

Morphological and Ultrastructural Observation of Blastocystis hominis

HE Ni1;ZHANG Yue-qing1;HONG Ming-li 2;CONG Min 2   

  1. Beijing Tropical Medicine Research Institute;Beijing 100050
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-06-30 Published:2001-06-30

摘要:   目的 观察人芽囊原虫的形态与超微结构。 方法 对人芽囊原虫培养 4~ 5 d的培养物进行多种染色后置光镜下观察形态结构 ,同时经过 4%的戊二醛固定处理后在透射电镜下观察超微结构。 结果 人芽囊原虫形态有空泡型、颗粒型、阿米巴型、复分裂型及包囊型等。分裂方式有二分裂方式及孢子分裂方式。透射电镜下可见虫体内含有细胞核、线粒体、粗面内质网、脂滴和溶酶体等细胞器 ,泡状结构内含有糖原颗粒。 结论 人芽囊原虫空泡型的泡状结构可能与储留排泄物有关 ,阿米巴型可能系该虫的致病类型。

关键词: 人芽囊原虫, 形态, 超微结构

Abstract:  Objective To observe the morphology and ultrastructure of Blastocystis hominis. Methods Morphological observation was made with 4-5 days cultured B.hominis by light microscopy, and similar material fixed with 4% glutaraldehyde was used for transmission electron microscopy. Results Several forms of B.hominis were observed including vacuolar, \{granular\}, amebic, multifission and cystic forms. The multiplication patterns of B.hominis included both binary fission and sporogony. Under transmission electron microscope, the nuclei, mitochondria, rough endoplasmic reticula and lysomes were observed in addition to lipid droplets in its cytoplasm, and glycogen in the central vacuole. Conclusion The central vacuole of vacuolar form may be related to the storage of the excreta. The amebic form of B.hominis might be pathogenic.

Key words: Blastocystis hominis, morphology, ultrastructure