南昌市HIV/AIDS患者人芽囊原虫感染现况及基因型分析

doi:10.12140/j.issn.1000-7423.2021.01.008

中国寄生虫学与寄生虫病杂志 ›› 2021, Vol. 39 ›› Issue (1): 55-60.doi: 10.12140/j.issn.1000-7423.2021.01.008

南昌市HIV/AIDS患者人芽囊原虫感染现况及基因型分析

钱科¹(), 胡主花¹, 吴秀萍², 彭国华¹, 俞英昉², 储言红², 周显凤¹, 徐丹¹, 滕雪娇², 李辉³, 田利光²^,^*()

1 传染病预防控制国家重点实验室研究基地,江西省动物源与媒介生物性传染病重点实验室,南昌市疾病预防控制中心,南昌 330038
2 中国疾病预防控制中心寄生虫病预防控制所,国家热带病研究中心,世界卫生组织热带病合作中心,科技部国家级热带病国际联合中心,卫生部寄生虫病原与媒介生物学重点实验室,上海 200025
3 南昌市卫生健康委员会,南昌 330038

收稿日期:2020-09-28 修回日期:2020-11-25 出版日期:2021-02-28 发布日期:2021-03-10
通讯作者: 田利光
作者简介:钱科(1986-),男,硕士,公卫主治医师,从事寄生虫病预防控制研究。E-mail: qkqk2003@sina.com
基金资助:
国家自然科学基金(81473022);上海市卫生与计划生育委员会科研课题(291440499);国家重点研发计划(2016YFC1202700);国家重点研发计划(2016YFC1202701);江西省卫生与计划生育委员会科技计划(20186023);江西省科技厅重点研发项目(20192BBGL70008)

Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City

QIAN Ke¹(), HU Zhu-hua¹, WU Xiu-ping², PENG Guo-hua¹, YU Ying-fang², CHU Yan-hong², ZHOU Xian-feng¹, XU Dan¹, TENG Xue-jiao², LI Hui³, TIAN Li-guang²^,^*()

1 The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-origin and Vector-borne Diseases, Nanchang Center for Disease Control and Prevention, Nanchang 330038, China
2 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China
3 Nanchang Municipal Health Commission, Nanchang 330038, China

Received:2020-09-28 Revised:2020-11-25 Online:2021-02-28 Published:2021-03-10
Contact: TIAN Li-guang
Supported by:
National Natural Science Foundation of China(81473022);Scientific Research Project of Shanghai Municipal Health and Family Planning Commission(291440499);National Key Research and Development Program(2016YFC1202700);National Key Research and Development Program(2016YFC1202701);Science and Technology Program of Jiangxi Provincial Health and Family Planning Commission(20186023);Key R&D Project of Jiangxi Provincial Department of Science and Technology(20192BBGL70008)

摘要/Abstract

摘要：

目的分析南昌市HIV/AIDS患者人芽囊原虫的感染状况及基因型。方法 2016年5—9月采用横断面调查的方法对南昌市在册管理的HIV阳性者进行问卷调查。采集HIV/AIDS患者血样和粪样,血样用于检测CD4 ⁺ T淋巴细胞水平;提取粪便人芽囊原虫DNA基因组,PCR扩增人芽囊原虫小亚基核糖体核糖核酸（SSU rDNA）基因序列片段,对疑似阳性PCR产物进行基因测序,通过基因序列比对进行确诊,并构建进化树进行基因分型。结果共调查HIV阳性者505人,采集粪样505份、血样475份。HIV/AIDS患者人芽囊原虫的感染率为4.16%（21/505）,其中男性人芽囊原虫感染率为4.22%（18/427）,女性为3.85%（3/78）,两者感染率差异无统计学意义（P > 0.05）。来源于市辖各县区疾控中心美沙酮门诊、市传染病医院和市辖区各监狱医院的HIV/AIDS患者人芽囊原虫的感染率分别为3.74%（4/107）、1.62%（5/308）和13.33%（12/90）,监狱医院来源的HIV/AIDS患者人芽囊原虫的感染率高于其他来源医疗机构（P < 0.05）。血清CD4 ⁺ T淋巴细胞计数> 400个/μl和≤ 400个/μl的HIV/AIDS患者人芽囊原虫感染率分别为3.29%（5/152）、4.64%（15/323）,差异无统计学意义（P > 0.05）。测序结果显示,南昌市HIV/AIDS患者感染的人芽囊原虫亚型为ST1、ST3和ST7,分别占4.76%（1/21）、90.48%（19/21）和4.76%（1/21）。结论南昌市辖区监狱医院来源的HIV/AIDS患者人芽囊原虫感染率较高;ST3亚型是南昌市HIV/AIDS患者主要感染基因型。

关键词: 人芽囊原虫, HIV/AIDS, 基因型, 南昌

Abstract:

Objective To determine the prevalence and genotype of Blastocystis hominis among HIV-positive individuals in Nanchang City. Methods A cross-sectional questionnaire survey was made among registered HIV-positive individuals in Nanchang City during May and September in 2016. Blood and fecal samples were collected. The level of CD4 ⁺ T cells in blood was detected. DNA genome was extracted from the fecal samples to amplify the small subunit ribosomal ribonucleic acid (SSU rDNA) of B. hominis. The positive products were sequenced and aligned to confirm the diagnosis, and phylogenic tree was constructed for genotyping. Results A total of 505 subjects were surveyed, and 505 fecal samples and 475 blood samples were collected. The infection rate of B. hominis was 4.16% (21/505) in the HIV/AIDS individuals, 4.22% (18/427) in the males and 3.85% (3/78) in the females, with no significant difference between males and females (P > 0.05). The infection rates in the HIV/AIDS populations from Methadone outpatient clinics of the Center for Disease Control, municipal infectious disease hospitals, and prison hospitals were 3.74% (4/107), 1.62% (5/308) and 13.33% (12/90), respectively. The infection rate in those from prison hospitals was significantly higher than the other two (P < 0.05). The infection rates in the HIV/AIDS populations with CD4 ⁺ T cell count > 400/μl and ≤ 400/μl were 3.29% (5/152) and 4.64% (15/323), respectively (P > 0.05). Sequencing results revealed prevalence of subtypes ST1 (4.76%, 1/21), ST3 (90.48%, 19/21), and ST7 (4.76%, 1/21) in Nanchang City. Conclusion There is high prevalence of B. hominis in HIV/AIDS cases from prison hospitals in Nanchang, and ST3 is the predominant subtype.

Key words: Blastocystis hominis, HIV/AIDS, Genotype characterization, Nanchang

中图分类号:

R382.9

钱科, 胡主花, 吴秀萍, 彭国华, 俞英昉, 储言红, 周显凤, 徐丹, 滕雪娇, 李辉, 田利光. 南昌市HIV/AIDS患者人芽囊原虫感染现况及基因型分析[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(1): 55-60.

QIAN Ke, HU Zhu-hua, WU Xiu-ping, PENG Guo-hua, YU Ying-fang, CHU Yan-hong, ZHOU Xian-feng, XU Dan, TENG Xue-jiao, LI Hui, TIAN Li-guang. Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2021, 39(1): 55-60.

图/表 2

参考文献 26

[1]	Stenzel DJ, Boreham PF. Blastocystis hominis revisited[J]. Clin Microbiol Rev, 1996,9(4):563-584. doi: 10.1128/CMR.9.4.563-584.1996 pmid: 8894352
[2]	Tan KSW. Blastocystis in humans and animals: new insights using modern methodologies[J]. Vet Parasitol, 2004,126(1/2):121-144. doi: 10.1016/j.vetpar.2004.09.017
[3]	Eroglu F, Koltas IS. Evaluation of the transmission mode of B. hominis by using PCR method[J]. Parasitol Res, 2010,107(4):841-845. doi: 10.1007/s00436-010-1937-4
[4]	Meloni D, Sanciu G, Poirier P, et al. Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy[J]. Parasitol Res, 2011,109(3):613-619. doi: 10.1007/s00436-011-2294-7
[5]	Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp.[J]. Clin Microbiol Rev, 2008,21(4):639-665. doi: 10.1128/CMR.00022-08 pmid: 18854485
[6]	Hu Y, Chen LY, Li YW. Intestinal protozoan infections among 451 patients with malignant cancer[J]. Mod Prev Med, 2015,42(20):3816-3817, 3833. (in Chinese)
	( 胡缨, 陈丽阳, 李艳文. 451例恶性肿瘤患者中肠道原虫感染情况研究[J]. 现代预防医学, 2015,42(20):3816-3817, 3833.)
[7]	Kurniawan A, Karyadi T, Dwintasari SW, et al. Intestinal parasitic infections in HIV/AIDS patients presenting with diarrhoea in Jakarta, Indonesia[J]. Trans R Soc Trop Med Hyg, 2009,103(9):892-898. doi: 10.1016/j.trstmh.2009.02.017 pmid: 19327806
[8]	Roberts T, Stark D, Harkness J, et al. Update on the pathogenic potential and treatment options for Blastocystis sp.[J]. Gut Pathog, 2014,6:17. doi: 10.1186/1757-4749-6-17 pmid: 24883113
[9]	Tian LG, Cheng GJ, Chen JX, et al. Survey on co-infection with HIV and intestinal parasites in high prevalence areas of HIV/AIDS, China[J]. Chin J Schisto Control, 2012,24(2):168-172. (in Chinese)
	( 田利光, 程国金, 陈家旭, 等. 我国农村HIV/AIDS高流行地区肠道寄生虫感染现况调查[J]. 中国血吸虫病防治杂志, 2012,24(2):168-172.)
[10]	Tian LG, Chen JX, Cheng GJ, et al. Survey on Blastocystis hominis infection in HIV positive individuals in Fuyang City, Anhui Province[J]. Chin J Schisto Control, 2012,24(3):303-306, 310. (in Chinese)
	( 田利光, 陈家旭, 程国金, 等. 安徽阜阳HIV阳性者合并人芽囊原虫感染及其影响因素分析[J]. 中国血吸虫病防治杂志, 2012,24(3):303-306, 310.)
[11]	Khoshnood S, Rafiei A, Saki J, et al. Prevalence and genotype characterization of Blastocystis hominis among the baghmalek people in southwestern Iran in 2013—2014[J]. Jundishapur J Microbiol, 2015,8(10):e23930. doi: 10.5812/jjm.23930 pmid: 26587213
[12]	Zali MR, Mehr AJ, Rezaian M, et al. Prevalence of intestinal parasitic pathogens among HIV-positive individuals in Iran[J]. Jpn J Infect Dis, 2004,57(6):268-270. pmid: 15623953
[13]	Roberts T, Stark D, Harkness J, et al. Update on the pathogenic potential and treatment options for Blastocystis sp.[J]. Gut Pathog, 2014,6:17. doi: 10.1186/1757-4749-6-17 pmid: 24883113
[14]	Xie RH, Chen GX, Ouyang SS. Analysis status of intestinal parasite infection among HIV/AIDS patients in Hengyang[J]. Chin J Immunol, 2015,31(5):695-697. (in Chinese)
	( 谢荣华, 陈高翔, 欧阳珊珊. 衡阳地区HIV/AIDS患者合并肠道寄生虫感染现状分析[J]. 中国免疫学杂志, 2015,31(5):695-697.)
[15]	Tian LG, Wang TP, Lv S, et al. HIV and intestinal parasite co-infections among a Chinese population: an immunological profile[J]. Infect Dis Poverty, 2013,2:18. doi: 10.1186/2049-9957-2-18 pmid: 23971713
[16]	Stensvold R, Brillowska-Dabrowska A, Nielsen HV, et al. Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction[J]. J Parasitol, 2006,92(5):1081-1087. doi: 10.1645/GE-840R.1 pmid: 17152954
[17]	Alfellani MA, Taner-Mulla D, Jacob AS, et al. Genetic diversity of Blastocystis in livestock and zoo animals[J]. Protist, 2013,164(4):497-509. doi: 10.1016/j.protis.2013.05.003
[18]	Kesuma Y, Firmansyah A, Bardosono S, et al. Blastocystis ST-1 is associated with irritable bowel syndrome-diarrhoea (IBS-D) in Indonesian adolescences[J]. Parasite Epidemiol Control, 2019,6:e00112. doi: 10.1016/j.parepi.2019.e00112 pmid: 31528737
[19]	Singh A, Priyadarshi K, Rai T, et al. A case report of Blastocystis infection and Steven Johnson’s syndrome[J]. Trop Biomed, 2019,36(4):987-992. pmid: 33597468
[20]	Zhou YL, Wu L, Yin Q, et al. Infections of Clostridium difficile, Blastocystis hominis and Cryptosporidium spp. in patients with chronic diarrhea[J]. Chin J Parasitol Parasit Dis, 2019,37(6):723-726. (in Chinese)
	( 周亚玲, 吴亮, 阴晴, 等. 慢性腹泻患者艰难梭菌、人芽囊原虫和隐孢子虫感染的研究[J]. 中国寄生虫学与寄生虫病杂志, 2019,37(6):723-726.)
[21]	de Melo GB, Malta FDM, Maruta CW, et al. Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil[J]. Parasite Epidemiol Control, 2019,7:e00124. doi: 10.1016/j.parepi.2019.e00124 pmid: 31872093
[22]	Kök M, Çekin Y, Çekin AH, et al. The role of Blastocystis hominis in the activation of ulcerative colitis[J]. Turk J Gastroenterol, 2019,30(1):40-46. doi: 10.5152/tjg.2018.18498 pmid: 30644363
[23]	Stensvold CR. Blastocystis: genetic diversity and molecular methods for diagnosis and epidemiology[J]. Trop Parasitol, 2013,3(1):26-34. doi: 10.4103/2229-5070.113896 pmid: 23961438
[24]	Teng XJ, Chu YH, Zhai CC, et al. The epidemiological characteristics and influencing factors for Blastocystis hominis infection among human immunodeficiency virus seropositive individuals in Tengchong of Yunnan Province[J]. Chin J Parasitol Parasit Dis, 2018,36(2):129-134. (in Chinese)
	( 滕雪娇, 储言红, 翟铖铖, 等. 云南腾冲人类免疫缺陷病毒携带者人芽囊原虫感染的流行病学特征及影响因素分析[J]. 中国寄生虫学与寄生虫病杂志, 2018,36(2):129-134.)
[25]	Alfellani MA, Stensvold CR, Vidal-Lapiedra A, et al. Variable geographic distribution of Blastocystis subtypes and its potential implications[J]. Acta Trop, 2013,126(1):11-18. doi: 10.1016/j.actatropica.2012.12.011 pmid: 23290980
[26]	Stensvold CR, Clark CG. Current status of Blastocystis: a personal view[J]. Parasitol Int, 2016,65(6 pt b):763-771. doi: 10.1016/j.parint.2016.05.015 pmid: 27247124

南昌市HIV/AIDS患者人芽囊原虫感染现况及基因型分析

Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City

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