中国寄生虫学与寄生虫病杂志 ›› 1997, Vol. 15 ›› Issue (6): 362-365.

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日本血吸虫虫卵与旋毛虫肌蚴间相似抗原的研究

曾庆仁1; 陆予云2; 易新元1; 曾宪芳1; 汪世平1; 彭先楚1   

  1. 1 湖南医科大学寄生虫学教研室; 2 广东省从化卫生学校
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-12-28 发布日期:1997-12-28

NTIGENIC SIMILARITY BETWEEN SCHISTOSOMA JAPONICUM EGG AND TRICHINELLA SPIRALIS MUSCLE LARVA ANTIGEN

Zeng Qingren1; Lu Yuyun2; Yi Xinyuan1; Zeng Xianfang1; Wang Shiping1; Peng Xianchu1   

  1. 1 Departm ent of Parasitology; H unan Medical University; Changsha 410078 2 Hygienic School of Conghua; Guangdong; Conghua 510900
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-12-28 Published:1997-12-28

摘要: 目的 :分析日本血吸虫虫卵与旋毛虫肌蚴间共同抗原的相似程度。方法 :应用 EITB和 ELISA技术对此 2种寄生虫可溶性抗原进行了血清学交叉反应分析。结果 :EITB显示 :在Ts ML A中 ,4 4、 53、 60、 64、 66/ 70 k Da和约 10 0 k Da的抗原为 Sj IRS识别 ,而 31、 4 2、 4 6、 51/53和 58/ 60 k Da成分为 α- Sj EARS识别 ;在 Sj EA中 ,4 6、58和 64k Da抗原为 Ts IRS识别 ,58、60、64和 70 k Da成分为α- Ts MLARS识别。EL ISA显示 :在 Sj IRS中抗 Ts抗体滴度显著高于在Ts IRS中所含抗血吸虫卵的抗体滴度。结论 :日本血吸虫卵与旋毛虫肌蚴间存有较多的共同抗原决定簇 ,但两者间的相似程度有明显差异。

关键词: 日本血吸虫, 旋毛虫, 相似抗原, 免疫印迹试验

Abstract: AIM:To analyse the antigenic similarity between Schistosoma japonicum egg and Trichinella
spiralis
muscle larva(TsMLA). METHODS:Soluble egg antigen of S.japonicum (Sj EA) and soluble muscle larva antigen of T.spiralis (TsMLA) were analysed by EITB and ELISA techniques. RESULTS:EITB showed that:in TsMLA, the antigens
of 44, 53, 60, 64, 66/70 and 100 kDa were recognized by SjIRS, while 31, 42, 46, 51/53 and 58/60 kDa antigens byα- SjEARS; in SjEA, the antigens of 46, 58 and 64 kDa were recognized by TsIRS, while 58, 60, 64 and 70 kDa proteins by α-T sMLARS. ELISA showed that the levels of antibodies against T. spiralis larva antigens in SjIRS were higher than those of antibodies against S. japonicum egg antigens in TsIRS. CONCLUSION: SjEA and TsMLA shared common antigens mainly among 44-70 kDa, but there were also differences in antigenic similarity between both S.japonicum egg and T.spiralis muscle larva.

Key words: Schistosoma japonicum, Trichinella spiralis, comm on antigen, EITB