中国寄生虫学与寄生虫病杂志 ›› 1986, Vol. 4 ›› Issue (1): 18-20.

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安徽省疟疾流行区疟疾间接荧光抗体水平的检测

韩范,陈锡慰,杨炳贵,林珍,张兆松,赵慰先,周大钧,胡道行,刘永源   

  1. 南京医学院寄生虫学教研室; 南京医学院寄生虫学教研室; 南京医学院寄生虫学教研室; 南京医学院寄生虫学教研室; 南京医学院寄生虫学教研室; 南京医学院寄生虫学教研室; 南京市江浦县卫生防疫站; 南京市江浦县卫生防疫站; 南京市江浦县卫生防疫站
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1986-02-28 发布日期:2017-01-12

DETECTION OF MALARIAL ANTIBODY LEVEL BY INDIRECT FLUORESCENT ANTIBODY TEST IN AN ENDEMIC AREA OF ANHUI

  • Received:2017-01-12 Revised:2017-01-12 Online:1986-02-28 Published:2017-01-12

摘要: 1983年11月初,在安徽省和县卓庙乡新景大队大包村,以体外培养的恶性疟原虫(Pf)和猴体内的食蟹猴疟原虫(Pc)为抗原,进行间接荧光抗体试验,检测人群疟疾抗体水平。同时用厚血膜法调查原虫率。当用P_f抗原时,抗体阳性率为67.0%,用P抗原为81.9%。0~15岁的低年龄组,前者为41 8%,后者为62.7%。16岁以上用P_f抗原时为82.6%,P_f为92.7%,两者的阳性率都高。该组人群原虫率高达23.0%,其中单纯感染恶性疟者为16.3%,间日疟为5.6%,两者混合感染者为1.1%。调查结果证实:当地近年发生了比较严重的疟疾流行。

关键词: 抗体水平, 间接荧光, 疟疾流行, 恶性疟原虫, 安徽省, 原虫率, 抗体阳性率, 猴疟原虫, 混合感染, 间日疟原虫

Abstract: In November 1983, indirect fluorescent antibody test(IFA) was carried out to detect the malarial antibody level in the population of Dabao Village, He County, Anhui Province. In this test, both antigens of Plasmodium falciparum(Pf) cultured in vitro and Plasmodium cynomolgi(Pc) were used, in addition to routine thick blood smears.The IFA-positive rate for the investigated population of the village is 67.0% and 81.3% for Pf and PC antigens, respectively. In the age group 0-15, the rate is 41.8% for Pf antigen and 62.7% for PC. Above the age of 16, the rate is 82.6% for Pf and 92.7% for PC. The results indicated that the level of malarial antibodies in the population was rather high. Thick blood smear examination of 178 subjects showed that the positive rate was 23% among which 29 cases were infected with Pf(16.3%), 10 cases with Pv(5.6%) and 2 cases with mixed infection(11l%). It was concluded that the area must have had a recent episode of malaria.