中国寄生虫学与寄生虫病杂志 ›› 1985, Vol. 3 ›› Issue (3): 181-183.

• • 上一篇    下一篇

间接荧光抗体试验使用两种不同疟原虫抗原进行疟疾单次横向调查

黄文洲,罗曼珍,周铭贤,陈辅群,宣圣珍   

  1. 中国预防医学中心寄生虫病研究所; 中国预防医学中心寄生虫病研究所; 中国预防医学中心寄生虫病研究所; 安徽省卫生防疫站; 安徽省卫生防疫站
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1985-08-31 发布日期:2017-01-12

INDIRECT FLUORESCENT ANTIBODY TEST WITH TWO DIFFERENT ANTIGENS IN A SINGLE CROSS-SECTIONAL SURVEY OF MALARIA

  • Received:2017-01-12 Revised:2017-01-12 Online:1985-08-31 Published:2017-01-12

摘要: 本文应用恶性疟原虫与食蟹猴疟原虫抗原作间接荧光抗体试验于1983年对五个不同类型疟区人群进行单次横向调查,同时以寄生虫学调查作对照。用平均每视野(10×40)含149.5个裂殖体的食蟹猴疟原虫抗原片与含124.4个体外培养的恶性疟原虫裂殖体的抗原片同时进行间接荧光抗体试验检测疟疾抗体。血清学测定的结果与血检相一致,而恶性疟原虫抗原与食蟹猴疟原虫抗原测出的抗体阳性率与GMRT,分别与该地恶性疟与间日疟流行水平和传播强度呈正相关。表明在恶性疟与间日疟混合流行地区,可以使用此两种抗原作间接荧光抗体试验进行血清流行病学调查,以了解恶性疟与间日疟的流行状况。

关键词: 疟原虫抗原, 间接荧光, 抗体试验, 疟疾感染, 恶性疟原虫, 间日疟, 血清流行病学调查, 抗体阳性率, 年发病率, 食蟹猴

Abstract: A single cross-sectibnal survey was conducted by IFAT with both P., falciparum and P. cynomolgi antigens in five different endemic areas of malaria in 1983. Parasito-logical examination was done simultaneously for comparison. Antigen slides containing either 149.5 schizonts of P. cynomolgi or 124.4 schizonts of P. falciparum per field (10 X 40) were used to detect the antibodies against malaria by IFAT at the same time. The results of serological assessment coincide well with those of blood examinations, the positive rates of antibodies and the GMRT level being proportionally related to the malaria endemicity and the transmission intensity of P.falciparum and; P. vivax in the areas studied. It suggests that both antigens for IFAT, could be used in sero-epidemsological investigation to determine the endemicity of falciparum and vivax malaria in areas of coexistence.